A septal chromosome segregator protein evolved into a conjugative DNA-translocator protein

Sepúlveda, Edgardo; Vogelmann, Jutta; Muth, Günther

doi:10.4161/mge.1.3.18066

Cited by 18 publications

(15 citation statements)

References 30 publications

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“…In a recent review by Thoma et al (2016), the conjugative transfer in Streptomyces was shown to require a DNA-translocase, namely TraB. TraB resembles a protein involved in chromosome segregation during cell division, FtsK (Sepulveda et al, 2011). Also, some plasmids or ICEs can be mobilized by other genetic elements.…”

Section: Introductionmentioning

confidence: 98%

Regulation of conjugative transfer of plasmids and integrative conjugative elements

Bañuelos-Vazquez

Tejerizo

Brom

2017

Plasmid

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Section: Introductionmentioning

confidence: 98%

Regulation of conjugative transfer of plasmids and integrative conjugative elements

Bañuelos-Vazquez

Tejerizo

Brom

2017

Plasmid

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“…Another type of conjugative DNA translocation machinery appears to have evolved in the order of Actinomycetales, it appears to be unique to conjugative plasmids and ICEs of multicellular bacteria belonging to this group of high G + C G+ bacteria (24). Their conjugative system resembles the machinery that promotes the segregation of chromosomal DNA during bacterial cell division and sporulation and requires a single FtsK-homologous protein to transfer doublestranded DNA to the recipient cell (24,25,26). Key factors of both translocation systems have been identified and the first models for conjugative plasmid transfer in G+ bacteria via both distinct modes have been presented (17,25,27,28,29).…”

Section: Introductionmentioning

confidence: 99%

Conjugation in Gram-Positive Bacteria

et al. 2014

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Conjugative transfer is the most important means of spreading antibiotic resistance and virulence factors among bacteria. The key vehicles of this horizontal gene transfer are a group of mobile genetic elements, termed conjugative plasmids. Conjugative plasmids contain as minimum instrumentation an origin of transfer (oriT), DNA-processing factors (a relaxase and accessory proteins), as well as proteins that constitute the trans-envelope transport channel, the so-called mating pair formation (Mpf) proteins. All these protein factors are encoded by one or more transfer (tra) operons that together form the DNA transport machinery, the Gram-positive type IV secretion system. However, multicellular Gram-positive bacteria belonging to the streptomycetes appear to have evolved another mechanism for conjugative plasmid spread reminiscent of the machinery involved in bacterial cell division and sporulation, which transports double-stranded DNA from donor to recipient cells. Here, we focus on the protein key players involved in the plasmid spread through the two different modes and present a new secondary structure homology-based classification system for type IV secretion protein families. Moreover, we discuss the relevance of conjugative plasmid transfer in the environment and summarize novel techniques to visualize and quantify conjugative transfer in situ.

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“…S. coelicolor contains 25 statistically distributed clt pSVH1 ‐like sequences. As TraB has been demonstrated to bind in vitro [45] and in vivo (unpublished results) to clc sites, transfer of chromosomal genes might be mediated by the interaction of TraB with chromosomal clc sites [48]. This is in contrast to the well characterized conjugation systems (e.g.…”

Section: Impact Of Lateral Gene Transfer On the Evolution And Diversimentioning

confidence: 92%

Synthetic Biology of secondary metabolite biosynthesis in actinomycetes: Engineering precursor supply as a way to optimize antibiotic production

et al. 2012

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a b s t r a c tActinomycetes are a rich source for the synthesis of medically and technically useful natural products. The genes encoding the enzymes for their biosynthesis are normally organized in gene clusters, which include also the information for resistance (in the case of antibacterial compounds), regulation, and transport. This facilitates the manipulation of such pathways by molecular genetic techniques. Recent advances in DNA sequencing and analytical chemistry revealed that not only new strains isolated from yet unexplored habitats, but also already known strains possess a large potential for the synthesis of novel compounds. Synthetic Biology now offers a new perspective to exploit this potential further by generating novel pathways, and thereby novel products, by combining different biosynthetic steps originating from different bacteria. The supply of precursors, which are subsequently incorporated into the final product, is often already organized in a modular manner in nature and may directly be exploited for Synthetic Biology. Here we report examples for the synthesis of building blocks and possibilities to modify and optimize antibiotic biosynthesis, exemplary for the synthesis of the manipulation of the synthesis of the glycopeptide antibiotic balhimycin.

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A septal chromosome segregator protein evolved into a conjugative DNA-translocator protein

Cited by 18 publications

References 30 publications

Regulation of conjugative transfer of plasmids and integrative conjugative elements

Regulation of conjugative transfer of plasmids and integrative conjugative elements

Conjugation in Gram-Positive Bacteria

Synthetic Biology of secondary metabolite biosynthesis in actinomycetes: Engineering precursor supply as a way to optimize antibiotic production

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