A Separation‐Sensing Membrane Performing Precise Real‐Time Serum Analysis During Blood Drawing

Abstract

Dynamic and on-site analysis of serum from human blood is crucial, however,state-of-the-art blood-assaymethods can only collect single or discrete data of physiological analytes;t hus,t he online reports of the dynamic fluctuation of key analytes remains ag reat challenge.H ere,w ep ropose anovel separation-sensing membrane by constructing aheterogeneous-nanostructured architecture,w herein as urface nanoporous layer continuously extracts serum, while the biosensing nanochannels underneath dynamically recognise biotargets, therebya chieving ac ontinuous testing of vital clinical indices as blood is drawn. By precisely controlling the pore structure and nanoshape of biosensing crystals,this membrane achieved accurate and online glucose and lactate monitoring in patients with avariety of medical conditions within 1min, which is one order of magnitude faster than state-of-the-art techniques. Moreover,v arious kinds of bio-recognisers can be introduced into this membrane to accurately detect glutamate,t ransaminase,and cancer biomarkers.

Membranes for the life sciences and their future roles in medicine

“…Recently, a hollow fiber membrane has aroused great attention for the introduction of analytical functions to membranes due to its hollow structure, which facilitates the construction of a signal circuit. Our group first proposed a separation-sensing membrane to integrate the separation process and electrochemical biosensing process into a single hollow fiber membrane that can extract serum in situ and simultaneously recognize the target biomolecules during blood drawing [144] . The heterogeneous nanostructure of this membrane exhibited separation ability on the porous surface layer and biosensing functions in the inner membrane channel (shown in Fig.…”

Scalable Printing of Prussian Blue Analogue@Au Edge-Rich Microcubes as Flexible Biosensing Microchips Performing Ultrasensitive Sucrose Fermentation Monitoring

“…Electrochemical biosensing technology possesses advantages including fast response, high selectivity, and low cost; hence, it has been widely applied in the clinical diagnosis of various physiological indices, such as glucose. − Nevertheless, because the target concentration in fermentation is much higher than that in body fluids, it is difficult to adopt the clinical biosensor directly for the test of the fermentation broth. , Considering that sucrose is hydrolyzed into glucose and fructose by sucrase in the intestinal cavity in the process of human absorption, , thereby, this metabolic pathway can be adopted to design an enzymatic reaction for the construction of an ultrasensitive and wide-range sucrose biosensor to match the fermentation process. Prussian blue (PB) and its analogues (PBAs) have been proved to possess ultrahigh electrocatalysis and specificity to enzymatic reactions in our previous studies. , Therefore, we selected the Cu–Co PBA as the core material for the sucrose recognition.…”

In Situ Construction of Oriented Pt‐PANI Needle‐Like Nanoarrays‐Based Label‐Free Aptasensor for Ultrafast and Ultrasensitive Recognition of Cardiac Troponin I

“…Among them, noble metals including Au, Ag, and Pt [ 15 ] are most applied on account of their high catalysis and abundant sites for DNA immobilization. [ 16 ] Nevertheless, their crystals especially in nanoscale prefer aggregation [ 17 ] together to cause poor distribution to affect catalysis and conductivity for DNA signal generation and transfer which directly determine both sensitivity and analysis time of the cTnI detection.…”