A Sensitivity Analysis of Methodological Variables Associated with Microbiome Measurements
Samuel P. Forry,
Stephanie L. Servetas,
Jennifer N. Dootz
et al.
Abstract:The experimental methods employed during metagenomic sequencing analyses of microbiome samples significantly impact the resulting data and typically vary substantially between laboratories. In this study, a full factorial experimental design was used to compare the effects of a select set of methodological choices (sample, operator, lot, extraction kit, variable region, reference database) on the analysis of biologically diverse stool samples. For each parameter investigated, a main effect was calculated that … Show more
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