A sensitive thrombin-linked sandwich immunoassay for protein targets using high affinity phosphorodithioate modified aptamer for thrombin labeling

Wang, Chao; Sun, Yang; Zhao, Qiang

doi:10.1016/j.talanta.2019.120280

Cited by 19 publications

(13 citation statements)

References 39 publications

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“…An aptamer holding the sequence of d(GGSTSTSGGTGTGGSTSTSGG) was modified by thio substitutions at the loop regions, which resulted in a high stability against nucleases and a low hydrolysis rate in circulation while the anti-thrombin potency remained. Recently, Wang et al introduced phosphorodiothioate (PS2) substitutions to stabilize phosphodiester bonds . PS2 modifications could also improve the binding between aptamers and their targets.…”

Section: Aptamer Modifications For Translating Into Clinical Candidatesmentioning

confidence: 99%

“…Recently, Wang et al introduced phosphorodiothioate (PS2) substitutions to stabilize phosphodiester bonds. 77 PS2 modifications could also improve the binding between aptamers and their targets. PS2-modified aptamers possess a 1000-fold improved target binding affinity compared with an unmodified original aptamer (Figure 5).…”

Section: Aptamer Modifications For Translating Into Clinical Candidatesmentioning

confidence: 99%

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Recent Progress in Aptamer Discoveries and Modifications for Therapeutic Applications

Zhang

Pan

et al. 2020

ACS Appl. Mater. Interfaces

352

267

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Aptamers are oligonucleotide sequences with a length of about 25−80 bases which have abilities to bind to specific target molecules that rival those of monoclonal antibodies. They are attracting great attention in diverse clinical translations on account of their various advantages, including prolonged storage life, little batch-to-batch differences, very low immunogenicity, and feasibility of chemical modifications for enhancing stability, prolonging the half-life in serum, and targeted delivery. In this Review, we demonstrate the emerging aptamer discovery technologies in developing advanced techniques for producing aptamers with high performance consistently and efficiently as well as requiring less cost and resources but offering a great chance of success. Further, the diverse modifications of aptamers for therapeutic applications including therapeutic agents, aptamer−drug conjugates, and targeted delivery materials are comprehensively summarized.

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Section: Aptamer Modifications For Translating Into Clinical Candidatesmentioning

confidence: 99%

Section: Aptamer Modifications For Translating Into Clinical Candidatesmentioning

confidence: 99%

Recent Progress in Aptamer Discoveries and Modifications for Therapeutic Applications

Zhang

Pan

et al. 2020

ACS Appl. Mater. Interfaces

352

267

View full text Add to dashboard Cite

show abstract

“…In addition to the referenced signal amplification approaches, various specific aptamers have been utilized to detect bacteria. Aptamers are single-stranded oligonucleotides that display specific affinity toward their targets, including whole cells, bacteria, proteins, and small molecules. − Except for S. typhimurium , Vibrio parahemolyticus ( VP ) is another popular foodborne bacterium. − These two types of bacteria usually coexist in food samples. − Therefore, it is necessary to develop an on-site and sensitive strategy for the simultaneous analysis of different pathogenic bacteria. To enhance the detection efficiency, a dual-mode assay combining qualitation and quantitation is a desirable alternative.…”

Section: Microscale Systems “See” In Bacteriamentioning

confidence: 99%

What the Microscale Systems “See” In Biological Assemblies: Cells and Viruses?

et al. 2021

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“…In the literature, there are many methods and techniques to detect thrombin, such as fluorescence, colorimetric, and electrochemical. Techniques such as immunoassay-based and radioassay-based methods are in use as a benchmark. , However, the aforementioned methods are time-consuming, high-cost, and affected by various pHs and temperatures . For instance, immunoassay uses natural antibodies, which are temperature-dependent and suffer from denaturation and a short shelf-life. ,, Thus, one should think about stable, robust, selective, and sensitive alternatives with low-cost precursors.…”

Section: Introductionmentioning

confidence: 99%

Nanozyme and Stimulated Fluorescent Cu-Based Metal–Organic Frameworks (Cu-MOFs) Functionalized with Engineered Aptamers as a Molecular Recognition Element for Thrombin Detection in the Plasma of COVID-19 Patients

Ali

Omer

2022

ACS Omega

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An essential tool in the management and control of the COVID-19 pandemic is the development of a fast, selective, sensitive, and inexpensive COVID-19 biomarkers detection method. Herein, an ultrasensitive and labelfree biosensing strategy was described for the colorimetric and fluorimetric detection of thrombin. A dual-mode aptasensing method based on integrating engineered ssDNA with a stimulated fluorescent enzyme-mimetic copper-based metal−organic framework (Cu-MOF) as a molecular recognition element for thrombin was investigated. Cu-MOFs displayed stimulated fluorescence and enzyme-mimetic peroxidase activities that oxidize the chromogenic colorless substance TMB to blue-colored oxTMB. The thrombin-based aptamer (ssDNA) can be immobilized on the Cu-MOF surface to form a functionalized composite, ssDNA/MOF, and quench the stimulated fluorescence emission and the enzymatic activity of the Cu-MOF. Later, addition of thrombin recovers the fluorescence and enzymatic activity of the MOF. Thus, a turn-on colorimetry/fluorimetry aptasensing probe was designed for the detection of thrombin. Based on colorimetric assay, 350 pM was recorded as the lower limit of detection (LOD), while based on the fluorescence mode, 110 fM was recorded as the LOD (when S/N = 3). The label-free aptasensing probe was used successfully for the detection of thrombin in COVID-19 patients with satisfactory recoveries, 95−98%. Since the detection time of our aptasensor is relatively rapid (45 min) and due to the low-cost precursors and easy-to-operate characteristics, we believe that it has great potential to be used in point-of-care testing (POCT).

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A sensitive thrombin-linked sandwich immunoassay for protein targets using high affinity phosphorodithioate modified aptamer for thrombin labeling

Cited by 19 publications

References 39 publications

Recent Progress in Aptamer Discoveries and Modifications for Therapeutic Applications

Recent Progress in Aptamer Discoveries and Modifications for Therapeutic Applications

What the Microscale Systems “See” In Biological Assemblies: Cells and Viruses?

Nanozyme and Stimulated Fluorescent Cu-Based Metal–Organic Frameworks (Cu-MOFs) Functionalized with Engineered Aptamers as a Molecular Recognition Element for Thrombin Detection in the Plasma of COVID-19 Patients

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