A Sensitive Small-Volume UV/Vis Flow Cell and Total Absorbance Detection System for Micro-HPLC

Aiello, Mauro; McLaren, R.

doi:10.1021/ac000943e

Cited by 14 publications

(13 citation statements)

References 14 publications

(11 reference statements)

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“…[1][2][3][4] As an example, smaller sample volumes allow reduced dead volumes in flowing systems and thereby limit the spread of bands flowing through a detection cell. 5 We observed enhanced detection in a previous series of studies [6][7][8] of the flow of supercritical fluids in microcapillary columns (250 mm i.d.) packed with 5 mm diameter silica particles.…”

Section: Introductionmentioning

confidence: 79%

Enhanced Fluorescence in a Scattering Medium

2021

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Often only small amounts of sample are available for spectroscopic analytical determinations. This work investigates the enhancement of signal in columns packed with silica particles. We propose that silica particles cause the light to scatter through the column, effectively increasing optical path length. Packed columns are shown to be effective with fluorescence spectroscopy, but results were inconclusive with absorbance spectroscopy.

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Section: Introductionmentioning

confidence: 79%

Enhanced Fluorescence in a Scattering Medium

2021

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“…On-line coupling of the miniaturized sample preparation technique and the microcolumn separations allow the hyphenated system to be further miniaturized; indicating the future possibility of developing a microscale total analytical system (micro-TAS) [131 -139], although the down-sizing of the detection device [140,141] should be additionally accomplished.…”

Section: Two-dimensional (2d) Comprehensive Lcmentioning

confidence: 99%

Capillary columns in liquid chromatography: between conventional columns and microchips

Saito

Jinno

Greibrokk³

2004

J of Separation Science

105

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Liquid chromatography on columns with small internal diameters has been reviewed as the intermediate technique between conventional liquid chromatography and microchip separations. The development of micro column separations in the early years has been described, starting with the papers of Horváth and co-workers and Ishii and co-workers, continuing into the first part of the eighties, then making a leap in time to recent innovations with small-bore columns. Based on internal diameters a classification of the different analytical HPLC columns has been suggested. The advantages of small-bore columns have been discussed, with particular emphasis on the advantage of coupling to concentration sensitive detectors when the sample amount is limited. Open tubular columns are treated as a part of the historic background. The recent developments include a brief look into the current status of monolithic columns, the use of packed nano columns and micro columns with electrospray mass spectrometry, and the potential of two-dimensional comprehensive liquid chromatography. Finally, the coupling of sample preparation to analytical columns and the future applications of the novel technological improvements to the microchip separation methods have been discussed.

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“…acrolein, glutaraldehyde, and dialdehydes) based on the cartridge method has encountered stability problems with the DNPH hydrazones [11]. Modifications in analytical procedure in terms of the type of adsorbents, the derivatization reagent, as well as the utilization of other analytical measurement principles have been reported in recent years [1,3,4,[13][14][15][16][17]. Owing to interference, ozone has to be removed by employing a KI denuder or scrubber upstream to the carbonyl collection.…”

Section: Introductionmentioning

confidence: 99%