A sensitive, simple and robust LC–MS/MS method for the simultaneous quantification of seven androgen- and estrogen-related steroids in postmenopausal serum

Ke, Yuyong; Bertin, Jonathan; Gonthier, Renaud; Simard, Jean-Nicolas; Labrie, Fernand

doi:10.1016/j.jsbmb.2014.08.015

Cited by 122 publications

(84 citation statements)

References 25 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In the European Union pharmaceuticals authorization procedures include Comission directives, which contain a requirement to develop a strategic approach to the pollution of water by pharmaceutical substances. E2 and EE2 were newly added to the EU watch list of emerging pollutants Ke et al 2014, Skotnicka-Pitak et al 2008) and immunoassays (Franke et al 2011, Moraes et al 2015, Tan &Wei 2015, but chromatographic based methods with UV-VIS, fluorescence or electrochemical detection are also available (Popescu et al 2008, Xu et al 2013, Zou et al 2014. Estrogenic activity can be determined by biological monitoring approaches.…”

Section: Introductionmentioning

confidence: 99%

β-Estradiol and ethinyl-estradiol contamination in the rivers of the Carpathian Basin

Avar

Zrínyi²,

Maász

et al. 2016

Environ Sci Pollut Res

View full text Add to dashboard Cite

17β-estradiol (E2) and 17α-ethinyl estradiol (EE2), which are environmental estrogens have been determined with LC-MS in freshwater. Their sensitive analysis needs derivatisation and therefore is very hard to achieve in multiresidue screening. We analysed samples from all the large and some small rivers (River Danube, Drava, Mur, Sava, Tisza and Zala) of the Carpathian Basin and from Lake Balaton. Freshwater was extracted on solid phase and derivatised using dansyl-chloride. Separation was performed on a Kinetex XB-C18 column. Detection was achieved with a benchtop orbitrap mass spectrometer using targeted MS analysis for quantification. Limits of quantification were 0.05 ng/L (MS1) and 0.1 ng/L (MS/MS) for E2, and 0.001 ng/L (MS1) and 0.2 ng/L (MS/MS) for EE2. River samples contained n.d.-5.2 ng/L E2 and n.d.-0.68 ng/L EE2. Average levels of E2 and EE2 were 0.61 and 0.084 ng/L respectively in rivers, water courses and Lake Balaton together, but not counting city canal water. EE2 was less abundant, but it was still present in almost all of the samples. In beach water samples from Lake Balaton we measured 0.076-0.233 E2 and n.d.-0.133 EE2. A relative high amount of EE2 was found in river Zala (0.68 ng/L) and in Hévíz-Páhoki canal (0.52 ng/L), which are both in the catchment area of Lake Balaton (Hungary). HighlightsMethod is proposed for simultaneous determination of 17β-estradiol and 17α-ethinyl estradiol. Derivatization is necessary to reach low LOQ. Low ng/L presence of estrogens in selected European Rivers has been detected.

show abstract

Section: Introductionmentioning

confidence: 99%

β-Estradiol and ethinyl-estradiol contamination in the rivers of the Carpathian Basin

Avar

Zrínyi²,

Maász

et al. 2016

Environ Sci Pollut Res

View full text Add to dashboard Cite

show abstract

“…For example, estradiol is seen at the low pg/mL level in postmenopausal serum and serum testosterone is low at all ages in women [6][7][8][10][11][12][13]. With today's technology, it should be clearly stated that a properly validated LC-MS/MS assay is the only method recommendable for the accurate measurement of estrogens and their metabolites.…”

Section: Discussionmentioning

confidence: 99%

“…We could even share these services with many colleagues around the world. An additional advantage of the MS-based assay is the multiple analyte possibility of LC-MS/MS as shown by the simultaneous validated assay of seven free steroids [9].…”

Section: High Specificity Sensitivity and Precision Of Lc-ms/msmentioning

confidence: 99%

Why both LC–MS/MS and FDA-compliant validation are essential for accurate estrogen assays?

Labrie

Gonthier

et al. 2015

The Journal of Steroid Biochemistry and Molecular Biology

Self Cite

View full text Add to dashboard Cite

“…The hemolyzed serum was prepared by spiking serum with 2% whole blood. All these experiments intend to test the reliability and robustness of the method, as requested by the FDA guidelines for the bioanalytical method development and validation [22]. The acceptance criteria are that the measured values should be within the 15% bias range of the nominal value while the CV should be less than 15%.…”

Section: Matrix Effect Lipemic Effect and Hydrolysis Effectmentioning

confidence: 99%

Validated LC–MS/MS simultaneous assay of five sex steroid/neurosteroid-related sulfates in human serum

Dury¹,

Ke²,

Gonthier³

et al. 2015

The Journal of Steroid Biochemistry and Molecular Biology

Self Cite

View full text Add to dashboard Cite

A sensitive, simple and robust LC–MS/MS method for the simultaneous quantification of seven androgen- and estrogen-related steroids in postmenopausal serum

Cited by 122 publications

References 25 publications

β-Estradiol and ethinyl-estradiol contamination in the rivers of the Carpathian Basin

β-Estradiol and ethinyl-estradiol contamination in the rivers of the Carpathian Basin

Why both LC–MS/MS and FDA-compliant validation are essential for accurate estrogen assays?

Validated LC–MS/MS simultaneous assay of five sex steroid/neurosteroid-related sulfates in human serum

Contact Info

Product

Resources

About