A sensitive signal-off aptasensor for adenosine triphosphate based on the quenching of Ru(bpy)32+-doped silica nanoparticles electrochemiluminescence by ferrocene

Li, Meng; Yang, Hongmei; Ma, Chao; Zhang, Yan; Ge, Shenguang; Yu, Jinghua; Yan, Mei

doi:10.1016/j.snb.2013.10.020

Cited by 27 publications

(9 citation statements)

References 46 publications

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“…To circumvent the drawback of steric hindrance, aptasensors based on split aptamers were designed to improve the detection ability, which exhibited a well-enhanced effect by increasing signal-to-background ratio. − In the past years, some efforts have been initiated to combine split aptamers with versatile materials for the design of various electrochemical or fluorescent biosensors. Briefly, fluorescent biosensors can be generally categorized into following groups: signal-on , or signal-off , fluorescent methods, colorimetric methods, , chemiluminescence methods, − and other methods On the whole, these biosensors were all based on the sandwich assay, and split aptamers were simultaneously used to capture target and generate signal.…”

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confidence: 99%

Bivalent Aptasensor Based on Silver-Enhanced Fluorescence Polarization for Rapid Detection of Lactoferrin in Milk

Zhu

Jia

et al. 2017

Anal. Chem.

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Here we report a novel type of bivalent aptasensor based on silver-enhanced fluorescence polarization (FP) for detection of lactoferrin (Lac) in milk powder with high sensitivity and specificity. The novel two split aptamers were obtained from the aptamer reported in our previous SELEX (systematic evolution of ligands by exponential enrichment) selection, and their minimal structural units were optimized on the basis of their affinity and specificity. Also, dual binding sites of split aptamers were verified. The bivalent aptamers were modified to be linked with signal-molecule fluorescein isothiocyanate (FITC) and enhancer silver decahedral nanoparticles (AgNPs). The split aptamers could bind to different sites of Lac and assemble into a split-aptamers-target complex, narrowing the distance between AgNPs and FITC dye. As a result, AgNPs could produce a mass-augmented and metal-enhanced fluorescence (MEF) effect. In general, ternary amplification based on AgNPs, split aptamers, and the MEF effect all contributed to the significant increase of FP values. It was proved that the sensitivity of this assay was about 3 orders of magnitude over traditional aptamer-based homogeneous assays with a detection limit of 1.25 pM. Furthermore, this design was examined by actual milk powder with rapid and high-throughout detection.

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confidence: 99%

Bivalent Aptasensor Based on Silver-Enhanced Fluorescence Polarization for Rapid Detection of Lactoferrin in Milk

Zhu

Jia

et al. 2017

Anal. Chem.

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“… However, no obvious change was observed for the CV oxidation peak of Ru(bpy) 3 2+ at 1.15 V (Figure A, curves a and b). Meanwhile, the ECL peak intensity decreased dramatically (Figure B, curve b) because of the ECL quenching effect of FcMeOH eq . − …”

Section: Resultsmentioning

confidence: 99%

“…FcMeOH is an electroactive probe and also an efficient quencher for the ECL signal of Ru(bpy) 3 2+ in solution − and was thus used in the present work. After the addition of 1 mM FcMeOH to the buffer solution containing Ru(bpy) 3 2+ and DNA, a new pair of CV quasi-reversible peaks appeared at approximately 0.35 V at the CP-free MIP film electrodes (Figure A, curve b), which is attributed to the ferricinum/ferrocene redox couple (eq ).…”

Section: Resultsmentioning

confidence: 99%

“…When double-stranded natural DNA was added to the Ru(bpy) 3 2+ solution, enhanced CV and ECL peaks at approximately 1.15 V vs SCE were simultaneously observed at the MIP film electrodes after CP removal. Meanwhile, ferrocene methanol (FcMeOH) functioned as both the electroactive probe and the ECL quencher in the system. − Thus, with DNA, CP, and FcMeOH as the three inputs and the corresponding CV and ECL peak responses as multiple outputs, the 3-input/3-output and 3-input/5-output logic gates were established. Furthermore, some non-Boolean logic devices, such as an encoder, a decoder, and a demultiplexer, were also constructed on the basis of the same system with elaborate designs.…”

Section: Introductionmentioning

confidence: 99%

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Biomacromolecular Logic Devices Based on Simultaneous Electrocatalytic and Electrochemiluminescence Responses of Ru(bpy)₃²⁺at Molecularly Imprinted Polymer Film Electrodes

2015

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A series of biomacromolecular logic gates and functional devices with cyclic voltammetric (CV) and electrochemiluminescence (ECL) responses as output signals were established on the basis of molecularly imprinted polymer (MIP) film electrodes. The MIP films were electropolymerized on the surface of Au electrodes with o-phenylenediamine (OPD) as the monomer and chloramphenicol (CP) as the template molecule. The simultaneous CV and ECL signals of Ru(bpy) 3 2+ were significantly enhanced by the addition of natural DNA in solution at the MIP film electrodes after CP removal. The CV and ECL responses of the Ru(bpy) 3 2+ −DNA system at the MIP film electrodes were greatly influenced by CP removal and rebinding. Moreover, the addition of ferrocene methanol (FcMeOH) to the solution led to substantial quenching of the ECL signal and produced a new CV peak pair. On the basis of these results, 3-input/3-output and 3-input/5-output biomacromolecular logic gate systems were established with DNA, CP, and FcMeOH as inputs and the ECL responses at different levels or CV responses at different potentials as outputs. After an elaborate reconfiguration of inputs and outputs, the functional non-Boolean logic devices such as a 2-to-1 encoder, a 1-to-2 decoder, and a 1-to-2 demultiplexer were also constructed on the same platform.

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“…For example, Tan and co-workers prepared Ru(bpy) 3 2+ -doped silica (Ru-SiO 2 ) nanoparticles and used them as a fluorescent probe for leukemia cell identification . Furthermore, the Ru-SiO 2 nanoparticles were also used to construct ECL sensors for DNA analysis, , protein detection, immunoassays, , cell tests, , and so forth . Excellent analytical performances were obtained because each Ru-SiO 2 nanoparticle contained large amounts of Ru(bpy) 3 2+ molecules, which could greatly enhance the ECL signal and result in high sensitivity.…”

Section: Introductionmentioning

confidence: 99%

A Novel Electrochemiluminescence Immunosensor for the Analysis of HIV-1 p24 Antigen Based on P-RGO@Au@Ru-SiO₂ Composite

Zhou

Huang

et al. 2015

ACS Appl. Mater. Interfaces

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Ru(bpy)3(2+)-doped silica (Ru-SiO2) nanoparticles and gold-nanoparticle-decorated graphene (P-RGO@Au) were combined to form a P-RGO@Au@Ru-SiO2 composite. The composite was used to develop a novel sandwich-type electrochemiluminescence immunosensor for the analysis of HIV-1 p24 antigen. The composite worked as carrier to immobilize target antibody and to build a sandwich-type electrochemiluminescence immunosensor through an interaction between antigen and antibody. Importantly, high ECL signal could be obtained due to the large amounts of Ru(bpy)3(2+) molecules per Ru-SiO2 nanoparticle. The P-RGO@Au composite with good conductivity and high surface area not only accelerated the electron transfer rate but also improved the loading of both ECL molecules and capture antibody, which could further increase the ECL response and result in high sensitivity. Taking advantage of both Ru-SiO2 nanoparticles and the P-RGO@Au composite, the proposed immunosensor exhibited a linear range from 1.0 × 10(-9) to 1.0 × 10(-5) mg mL(-1) with a detection limit of 1.0 × 10(-9) mg mL(-1) for HIV-1 p24 antigen. The proposed ECL immunosensor was used to analyze HIV-1 p24 antigen in human serum, and satisfactory recoveries were obtained, indicating that the proposed method is promising for practical applications in the clinical diagnosis of HIV infection.

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A sensitive signal-off aptasensor for adenosine triphosphate based on the quenching of Ru(bpy)32+-doped silica nanoparticles electrochemiluminescence by ferrocene

Cited by 27 publications

References 46 publications

Bivalent Aptasensor Based on Silver-Enhanced Fluorescence Polarization for Rapid Detection of Lactoferrin in Milk

Bivalent Aptasensor Based on Silver-Enhanced Fluorescence Polarization for Rapid Detection of Lactoferrin in Milk

Biomacromolecular Logic Devices Based on Simultaneous Electrocatalytic and Electrochemiluminescence Responses of Ru(bpy)₃²⁺at Molecularly Imprinted Polymer Film Electrodes

A Novel Electrochemiluminescence Immunosensor for the Analysis of HIV-1 p24 Antigen Based on P-RGO@Au@Ru-SiO₂ Composite

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A sensitive signal-off aptasensor for adenosine triphosphate based on the quenching of Ru(bpy)32+-doped silica nanoparticles electrochemiluminescence by ferrocene

Cited by 27 publications

References 46 publications

Bivalent Aptasensor Based on Silver-Enhanced Fluorescence Polarization for Rapid Detection of Lactoferrin in Milk

Bivalent Aptasensor Based on Silver-Enhanced Fluorescence Polarization for Rapid Detection of Lactoferrin in Milk

Biomacromolecular Logic Devices Based on Simultaneous Electrocatalytic and Electrochemiluminescence Responses of Ru(bpy)32+at Molecularly Imprinted Polymer Film Electrodes

A Novel Electrochemiluminescence Immunosensor for the Analysis of HIV-1 p24 Antigen Based on P-RGO@Au@Ru-SiO2 Composite

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Biomacromolecular Logic Devices Based on Simultaneous Electrocatalytic and Electrochemiluminescence Responses of Ru(bpy)₃²⁺at Molecularly Imprinted Polymer Film Electrodes

A Novel Electrochemiluminescence Immunosensor for the Analysis of HIV-1 p24 Antigen Based on P-RGO@Au@Ru-SiO₂ Composite