A sensitive resonance Rayleigh light scattering method for alpinetin using gold nanorods probes

Abstract

A sensitive resonance Rayleigh light scattering (RLS) assay for alpinetin was developed based on alpinetin-modified gold nanorods (AuNRs). Alpinetin could interact with AuNRs and formed a new assembly by electrostatic attraction. In pH 7.4 Tris-HCl buffer solution, the assembly of alpinetin-AuNRs showed a sensitive RLS signal. Under optimum conditions, the magnitude of enhanced RLS intensity (ΔI ) was proportional to the concentration of alpinetin over the range 0.027-3.24 μg ml , with a detection limit of 1.79 ng ml (by 3σ). The developed RLS method was successfully applied to the detection of alpinetin in real or synthesized samples. Alpinetin recoveries were 90.4-108.7% with an RSD of 0.82-2.9% (n = 5) for Alpinia katsumadai samples, and 95.1-103.7% with an RSD of 0.28-3.9% (n = 5) for synthesized samples. The results showed that this new approach was convenient, reliable and sensitive.

Metabolic Profiling of Alpinetin in Rat Plasma, Urine, Bile and Feces after Intragastric Administration

“…Recently, it has been reported that alpinetin may be deemed as a good candidate for some possible treatment of liver injury and brain diseases [12,13]. Because of its multiple therapeutic activities, alpinetin is considered as a potential candidate drug for further clinical studies, and has recently attracted more and more attention from medical and pharmaceutical fields [14,15,16]. Therefore, a better understanding of the efficacy of alpinetin needs to elucidate its biological fate in the body.…”

Alpinetin: A Review of Its Pharmacology and Pharmacokinetics

“…For decades, alpinetin was also widely found in many other medicinal herbs and has been abstracted from Alpinia hainanensis K. Schum. [Zingiberaceae] (Rao and Lin, 1998;Liang et al, 2007;Liu et al, 2007;Wang et al, 2007;Liu and Wu, 2009;Li et al, 2010;Zou and Lu, 2011;Zhao et al, 2013;Bi et al, 2018), Alpinia mutica Roxb. [Zingiberaceae] (Malami et al, 2016;Malami et al, 2017a), Alpinia pinnanensis T. L. Wu & S. J.…”

“…The natural sources of alpinetin were listed in Table 1. The accurate methods for the separation and determination of alpinetin were developed by highperformance liquid chromatography (HPLC), combination of flow injection (FI)-micellar electrokinetic chromatography (MEKC), reverse micelle electrokinetic capillary chromatography (RMEKC), sensitive resonance Rayleigh light scattering (RLS) assay, and liquid chromatography-tandem mass spectrometry (LC-MS/MS) (Liu et al, 2007;Wang et al, 2007;Singh et al, 2015;Bi et al, 2018;Lorençoni et al, 2020).…”