A sensitive polyacrylamide disc gel method for detection of proteinases

Andary, Thomas J.; Dabich, Danica

doi:10.1016/0003-2697(74)90101-8

Cited by 34 publications

(4 citation statements)

References 19 publications

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“…BSA, a smaller molecule, is also frequently used incorporated in SDS-PAGE (Kelleher & Juliano 1984) or in radial diffusion in agar gel (Wikström et al 1981). Denatured hemoglobin is routinely used in assay systems for detecting proteolytic activity (Anson 1939) and also incorporated in SDS-PAGE (Andary & Dabich 1974).…”

Section: Discussionmentioning

confidence: 99%

Detection of extracellular proteases from microorganisms on agar plates

Vermelho

Meirelles²,

Lopes

et al. 1996

Mem. Inst. Oswaldo Cruz

103

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Section: Discussionmentioning

confidence: 99%

Detection of extracellular proteases from microorganisms on agar plates

Vermelho

Meirelles²,

Lopes

et al. 1996

Mem. Inst. Oswaldo Cruz

103

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“…The gels were treated with the following reagents: Coomassie brilliant blue (24) to visualize protein; periodic acid -Schiff s reagent (25) for glycoprotein; a 1% aqueous solution of toluidine blue for mucopolysaccharides; or GPNA in either 0.04 M or 0.1 M Tris buffer, pH 7.6, and 0.14% fast garnet GBC to visualize the p-naphthylamine released upon enzymic activity (26).…”

Section: Methodsmentioning

confidence: 99%

Characterization of the interaction between chymotrypsin and heparin

Twining¹,

Brecher²

1977

Can. J. Biochem.

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Heparin forms a complex with chymotrypsin which is active towards glutaryl-L-phenylalanine-p-nitroanilide (GPANA) and glutaryl-L-phenylalanine-beta-naphthylamide (GPNA) at pH 7.6. The activity of chymotrypsin towards GPANA at pH 7.6 is enhanced in the presence of heparin. Heparin does not bind at the active site of the enzyme since proflavin is not displaced from the active site of chymotrypsin upon complex formation. The heparin-chymotrypsin complex migrates under basic polyacrylamide disc gel electrophoresis conditions to a position intermediate between heparin and free chymotrypsin. The complex is dissociable under acidic polyacrylamide gel electrophoresis conditions. It is estimated that one to three molecules of heparin can bind to each chymotrypsin molecule on the basis of electrophoretic and enzymic activity data.

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“…For protein, the silver stain method of Merril et al (1980) was used where the Coomasie-blue stain was not sensitive enough. The method of Andary & Dabich (1974) was employed for staining proteinases, and that of Gomori (1953) and Higashi & Johnson (1986) was used to stain for esterases.…”

Section: P U R I F I C a T I O N A N D C H A R A C T E R I Z A T I O Nmentioning

confidence: 99%

Purification and characterization of extracellular proteinases excreted by a strain of Bacillus subtilis BS₂, isolated from fermented African locust bean ‘iru’

Aderibigbe

Odunfa

1988

Journal of Applied Bacteriology

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Proteinases were excreted by strains of Bacillus subtilis during fermentation of African locust bean cotyledons. Those excreted by one strain were purified and characterized by ammonium sulphate precipitation, ion‐exchange chromatography (IEC), gel filtration, inhibition tests and polyacrylamide gel electrophoresis (PAGE). Three proteinases and an esterase without proteolytic activity were identified. A serine proteinase which showed a high degree of hydrophobicity and a neutral proteinase were present. The third proteinase showed both proteolytic and esterolytic activities, and had multiple electrophoretic mobilities on polyacrylamide gel.

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A sensitive polyacrylamide disc gel method for detection of proteinases

Cited by 34 publications

References 19 publications

Detection of extracellular proteases from microorganisms on agar plates

Detection of extracellular proteases from microorganisms on agar plates

Characterization of the interaction between chymotrypsin and heparin

Purification and characterization of extracellular proteinases excreted by a strain of Bacillus subtilis BS₂, isolated from fermented African locust bean ‘iru’

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A sensitive polyacrylamide disc gel method for detection of proteinases

Cited by 34 publications

References 19 publications

Detection of extracellular proteases from microorganisms on agar plates

Detection of extracellular proteases from microorganisms on agar plates

Characterization of the interaction between chymotrypsin and heparin

Purification and characterization of extracellular proteinases excreted by a strain of Bacillus subtilis BS2, isolated from fermented African locust bean ‘iru’

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Product

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Purification and characterization of extracellular proteinases excreted by a strain of Bacillus subtilis BS₂, isolated from fermented African locust bean ‘iru’