A sensitive photoelectrochemical assay of miRNA-155 based on a CdSe QDs//NPC-ZnO polyhedra photocurrent-direction switching system and target-triggered strand displacement amplification strategy

Meng, Liang; Li, Yanmei; Yang, Ruirui; Zhang, Xiaohua; Du, Cuicui; Chen, Jinhua

doi:10.1039/c8cc09411j

Cited by 44 publications

(23 citation statements)

References 32 publications

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“…The target-induced recycling reaction resulted in a large amount of Zn 2+ , improving the detectability of the biosensor, achieving an LOD of 0.28 fM [136]. A new photoelectrochemical biosensor based on a photocurrent direction switching system and targettriggered SDA strategy was developed for miRNA detection with an excellent detection limit of about 49 aM [137]. A novel photoelectrochemical sensor for miRNA detection was also reported based on energy transfer between CdS:Mn dots and AuNPs, succeeding an LOD of 0.5 fM [138].…”

Section: Quantum Dots (Qds)mentioning

confidence: 99%

Nanotechnology in emerging liquid biopsy applications

Kalogianni

2021

Nano Convergence

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Liquid biopsy is considered as the most attractive alternative to traditional tissue biopsies. The major advantages of this approach lie in the non-invasive procedure, the rapidness of sample collection and the potential for early cancer diagnosis and real-time monitoring of the disease and the treatment response. Nanotechnology has dynamically emerged in a wide range of applications in the field of liquid biopsy. The benefits of using nanomaterials for biosensing include high sensitivity and detectability, simplicity in many cases, rapid analysis, the low cost of the analysis and the potential for portability and personalized medicine. The present paper reports on the nanomaterial-based methods and biosensors that have been developed for liquid biopsy applications. Most of the nanomaterials used exhibit great analytical performance; moreover, extremely low limits of detection have been achieved for all studied targets. This review will provide scientists with a comprehensive overview of all the nanomaterials and techniques that have been developed for liquid biopsy applications. A comparison of the developed methods in terms of detectability, dynamic range, time-length of the analysis and multiplicity, is also provided.

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Section: Quantum Dots (Qds)mentioning

confidence: 99%

Nanotechnology in emerging liquid biopsy applications

Kalogianni

2021

Nano Convergence

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“…The fabricated sensor exhibited desirable long-term stability with no significant change in photocurrent following storage for 10 days and excellent selectivity when incubated with a solution containing interfering agents (IGF-1 and C-peptide). Micro-RNA (miRNA-155) detection has been shown by introducing Au NP functionalized N-doped porous carbon ZnO polyhedra (NPC-ZnO) on CdSe QD based photoelectrode (Meng et al, 2019). Following, miRNA hybridization with a hairpin structure probe, Au NP functionalized NPC-ZnO was brought close to the hybridized double-stranded RNA by using second hairpin DNA structure (Figure 4D).…”

Section: Transduction Mechanismmentioning

confidence: 99%

“…(D) Schematic representation of the detection of miRNA-155 based on NPC-ZnO labeled target. Here, NPC-ZnO performs the role of electron scavenger, thus generating a signal-on response [Reprinted from Meng et al (2019) with permission from American Chemical Society].…”

Section: Transduction Mechanismmentioning

confidence: 99%

Affinity-Based Detection of Biomolecules Using Photo-Electrochemical Readout

et al. 2019

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Detection and quantification of biologically-relevant analytes using handheld platforms are important for point-of-care diagnostics, real-time health monitoring, and treatment monitoring. Among the various signal transduction methods used in portable biosensors, photoelectrochemcial (PEC) readout has emerged as a promising approach due to its low limit-of-detection and high sensitivity. For this readout method to be applicable to analyzing native samples, performance requirements beyond sensitivity such as specificity, stability, and ease of operation are critical. These performance requirements are governed by the properties of the photoactive materials and signal transduction mechanisms that are used in PEC biosensing. In this review, we categorize PEC biosensors into five areas based on their signal transduction strategy: (a) introduction of photoactive species, (b) generation of electron/hole donors, (c) use of steric hinderance, (d) in situ induction of light, and (e) resonance energy transfer. We discuss the combination of strengths and weaknesses that these signal transduction systems and their material building blocks offer by reviewing the recent progress in this area. Developing the appropriate PEC biosensor starts with defining the application case followed by choosing the materials and signal transduction strategies that meet the application-based specifications.

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“…MBs labeled with organic dyes generally have a broad emission band with a poor photostability and easy photobleaching, which are not desirable for the development of stable and multivariate detection probes. Quantum dot (QD) is a new type of fluorescence materials with distinct advantages of strong intensity, high stability, strong anti-bleaching ability, wide excitation spectrum, narrow emission spectrum, and adjustable emission wavelength [ [5] , [6] , [7] , [8] , [9] ]. The effective bandgap of QDs is increased with the decrease of particle radius, resulting in a blue shift of their absorption and emission [ 10 ].…”

Section: Introductionmentioning

confidence: 99%