A sensitive paper-based lateral flow immunoassay platform using engineered cellulose-binding protein linker fused with antibody-binding domains

Yang, Jong Min; Kim, Kyeong Rok; Jeon, Sangmin; Joon, Hyung; Kim, Chang Sup

doi:10.1016/j.snb.2020.129099

Cited by 22 publications

(17 citation statements)

References 36 publications

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“…Furthermore, we found comparable sensitivities, regarding hCG detection using NC-based LFAs (functionalized by anti-hCG scFv) or cellulose-based LFAs (functionalized by CBM-anti-hCG scFv) (Figure S7 ). This indicates that cellulose-based LFAs could reach similar sensitivities compared to common nitrocellulose-based LFAs, when using CBM-fused detection reagents, which is in accordance with recently published data 24 . Furthermore, we observed a dramatic loss of sensitivity of the solitary detection antibodies and antibody fragments on cellulose-based LFAs, compared to the CBM-fused counterparts (Fig.…”

Section: Discussionsupporting

confidence: 92%

“…Recently, a study addressing the applicability of a CBM-fused ZZ-domain for the colorimetric detection of DNA strands on cellulose particles was published by Rosa et al 23 . Additionally, a recently published study addressed the applicability of protein A-derived domains fused to different CBM domains, resulting in improved binding onto cellulose-paper used for lateral flow immuno assays 24 . However, this newly developed platform displayed several limitations including unwanted cross-reactivity to (detection-) antibodies from different species.…”

Section: Introductionmentioning

confidence: 99%

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Carbohydrate binding module-fused antibodies improve the performance of cellulose-based lateral flow immunoassays

Elter

Bock

Spiehl

et al. 2021

Sci Rep

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Since the pandemic outbreak of Covid-19 in December 2019, several lateral flow assay (LFA) devices were developed to enable the constant monitoring of regional and global infection processes. Additionally, innumerable lateral flow test devices are frequently used for determination of different clinical parameters, food safety, and environmental factors. Since common LFAs rely on non-biodegradable nitrocellulose membranes, we focused on their replacement by cellulose-composed, biodegradable papers. We report the development of cellulose paper-based lateral flow immunoassays using a carbohydrate-binding module-fused to detection antibodies. Studies regarding the protein binding capacity and potential protein wash-off effects on cellulose paper demonstrated a 2.7-fold protein binding capacity of CBM-fused antibody fragments compared to the sole antibody fragment. Furthermore, this strategy improved the spatial retention of CBM-fused detection antibodies to the test area, which resulted in an enhanced sensitivity and improved overall LFA-performance compared to the naked detection antibody. CBM-assisted antibodies were validated by implementation into two model lateral flow test devices (pregnancy detection and the detection of SARS-CoV-2 specific antibodies). The CBM-assisted pregnancy LFA demonstrated sensitive detection of human gonadotropin (hCG) in synthetic urine and the CBM-assisted Covid-19 antibody LFA was able to detect SARS-CoV-2 specific antibodies present in serum. Our findings pave the way to the more frequent use of cellulose-based papers instead of nitrocellulose in LFA devices and thus potentially improve the sustainability in the field of POC diagnostics.

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Section: Discussionsupporting

confidence: 92%

Section: Introductionmentioning

confidence: 99%

Carbohydrate binding module-fused antibodies improve the performance of cellulose-based lateral flow immunoassays

Elter

Bock

Spiehl

et al. 2021

Sci Rep

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“…The use of biodegradable components could also be desirable, even if most of the LFIAs for professional use (in the clinical field) must be incinerated due to possible biohazard. Notwithstanding the exceptional properties of the nitrocellulose as solid support for the LFIA platform, researchers [ 291 , 292 , 293 ] are trying to replace its use with the cellulose that, in addition to further reducing costs, would have a reduced impact on the environment because its production process requires less chemicals in comparison to the nitrocellulose production. Most recently, promising results have been obtained by Adrian Elter et al that developed cellulose-based LFIAs [ 294 ].…”

Section: Future Perspectivesmentioning

confidence: 99%

Ten Years of Lateral Flow Immunoassay Technique Applications: Trends, Challenges and Future Perspectives

Nardo

Chiarello

Cavalera

et al. 2021

Sensors

238

162

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The Lateral Flow Immunoassay (LFIA) is by far one of the most successful analytical platforms to perform the on-site detection of target substances. LFIA can be considered as a sort of lab-in-a-hand and, together with other point-of-need tests, has represented a paradigm shift from sample-to-lab to lab-to-sample aiming to improve decision making and turnaround time. The features of LFIAs made them a very attractive tool in clinical diagnostic where they can improve patient care by enabling more prompt diagnosis and treatment decisions. The rapidity, simplicity, relative cost-effectiveness, and the possibility to be used by nonskilled personnel contributed to the wide acceptance of LFIAs. As a consequence, from the detection of molecules, organisms, and (bio)markers for clinical purposes, the LFIA application has been rapidly extended to other fields, including food and feed safety, veterinary medicine, environmental control, and many others. This review aims to provide readers with a 10-years overview of applications, outlining the trends for the main application fields and the relative compounded annual growth rates. Moreover, future perspectives and challenges are discussed.

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“…The recombinant CBP31-BC protein was produced in Escherichia coli as previously described. [26] Recombinant E. coli BL21 (DE3) containing pET-CBP31-BC was grown in 400 mL of LB medium (0.5 % yeast extract, 1 % tryptophan, and 1 % NaCl) with 50 μg/mL ampicillin at 37 °C. When the culture reached an optical density of ∼0.8–0.9 at 600 nm (OD 600 ), 1 mM IPTG was added to induce the expression of CBP31-BC protein.…”

Section: Methodsmentioning

confidence: 99%

“…The protein adsorption capacity of the nitrocellulose membrane makes it difficult to control the orientation of immobilized antibodies [25] . In a previous study, we developed a cellulose membrane-based sensitive lateral flow immunoassay (LFIA) using a bifunctional fusion linker, CBP31-BC, composed of cellulose-binding domains (CBDs) and antibody-binding domains [26] . Due to oriented antibody immobilization on cellulose by CBP31-BC linker, the cellulose membrane-based LFIA showed a ∼10-fold higher sensitivity to prostate-specific antigens than nitrocellulose membrane-based conventional LFIAs.…”

Section: Introductionmentioning

confidence: 99%

A colorimetric lateral flow immunoassay based on oriented antibody immobilization for sensitive detection of SARS-CoV-2

Lee

Kim

et al. 2023

Sensors and Actuators B: Chemical

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A sensitive paper-based lateral flow immunoassay platform using engineered cellulose-binding protein linker fused with antibody-binding domains

Cited by 22 publications

References 36 publications

Carbohydrate binding module-fused antibodies improve the performance of cellulose-based lateral flow immunoassays

Carbohydrate binding module-fused antibodies improve the performance of cellulose-based lateral flow immunoassays

Ten Years of Lateral Flow Immunoassay Technique Applications: Trends, Challenges and Future Perspectives

A colorimetric lateral flow immunoassay based on oriented antibody immobilization for sensitive detection of SARS-CoV-2

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