1965
DOI: 10.1016/0009-8981(65)90091-4
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A sensitive method for the determination of xanthine oxidase activity

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1967
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Cited by 37 publications
(13 citation statements)
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“…XOA assay. In the standard assay, adapted from the method of Al-Khalidi et al, the clear supernatant was preincubated at 37°C for 40 minutes in a shaking water bath (21). A suitable amount of enzyme protein and unlabeled xanthine was added to a 40 mM Tris-HC1 buffer, pH 8.0, containing (8-I4C) xanthine (ICN Pharmaceutical Inc., Irvine, California, specific activity 53.5 mCi/mmole) in a final volume of 500 pl and incubated for an additional 30 minutes at 37°C in a Dubnoff metabolic shaker.…”
Section: Methodsmentioning
confidence: 99%
“…XOA assay. In the standard assay, adapted from the method of Al-Khalidi et al, the clear supernatant was preincubated at 37°C for 40 minutes in a shaking water bath (21). A suitable amount of enzyme protein and unlabeled xanthine was added to a 40 mM Tris-HC1 buffer, pH 8.0, containing (8-I4C) xanthine (ICN Pharmaceutical Inc., Irvine, California, specific activity 53.5 mCi/mmole) in a final volume of 500 pl and incubated for an additional 30 minutes at 37°C in a Dubnoff metabolic shaker.…”
Section: Methodsmentioning
confidence: 99%
“…To date, the activity of xanthine oxidase has been determined by HPLC method for measuring the conversion of pterin to isoxanthopterin in human plasma [11][12][13][14][15], radiometric [16], fluorometric [17,18] and ELISA [19] methods in serum. The serum/plasma activities were reported to be very low.…”
Section: Discussionmentioning
confidence: 99%
“…[2] developed a more sensitive method for its determination. The application of this radio-isotopic method established the value of SXO as a test for acute liver damage.…”
Section: Discussionmentioning
confidence: 99%