A sensitive method for the determination of hemoglobin in plasma

Vanzetti, G; Valente, Denise B.

doi:10.1016/0009-8981(65)90191-9

Cited by 20 publications

(4 citation statements)

References 3 publications

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“…We measured free Hb levels by HemoCue®, which utilizes the azide-methemoglobin method, which was initially described by Vanzetti in 1966 [ 21 ]. The device uses a chemically covered cuvette and dry reagents, such as sodium deoxycholate, sodium nitrite, and sodium nitrite deposited on the inner wall of the cuvette cavity to lyse the red cells and convert Hb into azide-methemoglobin [ 22 ].…”

Section: Discussionmentioning

confidence: 99%

Hemolysis Proceeds Urine Color Change in Patients Undergoing Open-Heart Surgery on Cardiopulmonary Bypass

Takeishi,

Yamamoto,

Sato

et al. 2023

Cureus

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Purpose: Red-colored urine often occurs in patients in the perioperative period who undergo cardiac surgery using cardiopulmonary bypass (CPB). This urine color change has been utilized for approximating hemolysis during CPB without a proven relationship for ongoing hemolysis. This case series study aimed to examine the relationship between plasma free hemoglobin (Hb) levels and quantified measures of urine color. Methods: Ten patients were enrolled in this study. Blood and urine were collected for analyses for the following time points: before surgery, two hours after the initiation of CPB, every 30 min during CPB thereafter, and 0, 2, 4, 12, and 24 hours after the completion of CPB. We measured free Hb in plasma and urine using the azide-methemoglobin method. Photographs of urine were obtained, and the luminance of the three basic colors (red/green/blue) was analyzed by quantitative luminance contrast analysis to find a correlation for hemolysis. Results: Median levels of plasma free Hb were 0.015 (0.010-0.080, n = 10) g/dL at baseline. During the CPB, increases in plasma free Hb levels were measured: median plasma free Hb levels were increased to 0.100 g/dL (0.020-0.240, p = 0.039, vs. baseline, n = 9) at two hours into CPB, median and range, respectively. In contrast, increases in urinary free Hb levels and/or urine color changes were measured only after cessation of CPB in nine patients. Conclusion: Urine color change or elevation of urinary free Hb levels followed the elevation of plasma free Hb levels with considerable delay.

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Section: Discussionmentioning

confidence: 99%

Hemolysis Proceeds Urine Color Change in Patients Undergoing Open-Heart Surgery on Cardiopulmonary Bypass

Takeishi,

Yamamoto,

Sato

et al. 2023

Cureus

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“…Significantly increased plasma haemoglobin levels have been recorded immediately after severe exercise (Chaplin et al. , 1961; Vanzetti & Valente, 1965) as well as after jogging (Davidson 1969). These old studies are worth repeating with the more refined methodology now available, and the PLHP may have a useful function in assessing the well‐being of athletes.…”

Section: Discussionmentioning

confidence: 99%

“…Our study indicates that this new HemoCue system would be reliable for detecting moderate to severe intravascular haemolysis with plasma haemoglobin above 100 mg/l, although it was unable to distinguish lesser degrees of lysis from the normal upper limit of 40 mg/l. Signi®cantly increased plasma haemoglobin levels have been recorded immediately after severe exercise (Chaplin et al, 1961;Vanzetti & Valente, 1965) as well as after jogging (Davidson 1969). These old studies are worth repeating with the more re®ned methodology now available, and the PLHP may have a useful function in assessing the well-being of athletes.…”

Section: Discussionmentioning

confidence: 99%

Use of a new HemoCue system for measuring haemoglobin at low concentrations

Morris¹,

Pont²,

Lewis³

2001

Clinical &Amp; Laboratory Haematology

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In many photometers and spectrometers used in routine practice Beer's law (relating absorbance to concentration) fails at low concentrations. A refinement of the HemoCue haemoglobin system (HemoCue AB, Sweden) provides a facility for accurate measurement of haemoglobin at the lower end of the scale. This study confirmed excellent linearity of the photometer response of the HemoCue Plasma/Low Hb photometer to haemoglobin concentration by direct measurement on whole blood samples, thus providing a reliable method when laboratory facilities are limited and where critical patient management may be dictated by the severity of anaemia. Furthermore, measurements on plasma and on samples of urine containing haemoglobin demonstrated the utility of the device for reliable quantification of plasma haemoglobin and haemoglobinuria, thus providing a useful method in investigating moderate or severe intravascular haemolysis. A study of plasma haemoglobin on stored samples of ethylenediamine tetraacetic acid (EDTA) blood showed that a small but measurable degree of lysis occurs after two days at room temperature and after four days when stored at 4 degrees C. This effect on the red cell count will reflect on the reliability of using such blood as a quality control preparation.

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“…Both methods give similar results. While this work was in progress, Vanzetti & Valente (1966) published details of a method for pregnanediol determination that also used periodate oxidation for removing glucuronide. They also used acidic conditions (pH4.5).…”

mentioning

confidence: 99%

Hydrolysis of Pregnanediol Glucuronide by Sodium Periodate

Wade¹

1967

Biochemical Journal

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A sensitive method for the determination of hemoglobin in plasma

Cited by 20 publications

References 3 publications

Hemolysis Proceeds Urine Color Change in Patients Undergoing Open-Heart Surgery on Cardiopulmonary Bypass

Hemolysis Proceeds Urine Color Change in Patients Undergoing Open-Heart Surgery on Cardiopulmonary Bypass

Use of a new HemoCue system for measuring haemoglobin at low concentrations

Hydrolysis of Pregnanediol Glucuronide by Sodium Periodate

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