A Sensitive Method for Quantifying Cytomegalic Endothelial Cells in Peripheral Blood from Cytomegalovirus-Infected Patients

Kas-Deelen, A.M.; Harmsen, M.; Maar, Eltjo F. de; Son, Willem J. van; The, T. H.

doi:10.1128/cdli.5.5.622-626.1998

Cited by 9 publications

(3 citation statements)

References 33 publications

(37 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The detection of cytomegalic endothelial cells in peripheral blood of patients is another means of monitoring active CMV infection. Through enrichment of endothelial cells in the mononuclear fraction by density centrifugation, endothelial cell-specific staining, and fluorescence-activated cell sorting of these cells, a method with 10-fold greater sensitivity than cytocentrifugation of the mononuclear cell fraction alone has recently been developed for quantification of cytomegalic endothelial cells by FCM (158). Belles-Isles et al (24) have also suggested using FCM to monitor CMV infections by monitoring the CD8 ϩ CD38 ϩ T-cell subset in kidney transplant recipients; this T-cell subpopulation usually increases during active viral infections.…”

Section: Direct Detectionmentioning

confidence: 99%

Applications of Flow Cytometry to Clinical Microbiology

Álvarez-Barrientos¹,

Arroyo

Cantón³

et al. 2000

Clin Microbiol Rev

190

View full text Add to dashboard Cite

SUMMARY Classical microbiology techniques are relatively slow in comparison to other analytical techniques, in many cases due to the need to culture the microorganisms. Furthermore, classical approaches are difficult with unculturable microorganisms. More recently, the emergence of molecular biology techniques, particularly those on antibodies and nucleic acid probes combined with amplification techniques, has provided speediness and specificity to microbiological diagnosis. Flow cytometry (FCM) allows single- or multiple-microbe detection in clinical samples in an easy, reliable, and fast way. Microbes can be identified on the basis of their peculiar cytometric parameters or by means of certain fluorochromes that can be used either independently or bound to specific antibodies or oligonucleotides. FCM has permitted the development of quantitative procedures to assess antimicrobial susceptibility and drug cytotoxicity in a rapid, accurate, and highly reproducible way. Furthermore, this technique allows the monitoring of in vitro antimicrobial activity and of antimicrobial treatments ex vivo. The most outstanding contribution of FCM is the possibility of detecting the presence of heterogeneous populations with different responses to antimicrobial treatments. Despite these advantages, the application of FCM in clinical microbiology is not yet widespread, probably due to the lack of access to flow cytometers or the lack of knowledge about the potential of this technique. One of the goals of this review is to attempt to mitigate this latter circumstance. We are convinced that in the near future, the availability of commercial kits should increase the use of this technique in the clinical microbiology laboratory.

show abstract

Section: Direct Detectionmentioning

confidence: 99%

Applications of Flow Cytometry to Clinical Microbiology

Álvarez-Barrientos¹,

Arroyo

Cantón³

et al. 2000

Clin Microbiol Rev

190

View full text Add to dashboard Cite

show abstract

“…These cells can be identified by cytocentrifugation of the mononuclear fraction of leukocytes onto glass slides followed by endothelial cell-specific staining. A method for quantification of CEC in peripheral blood has also been described (220). In patients with AIDS, the detection of CEC is associated with lack of anti-CMV treatment, emergence of drug-resistant CMV, insufficient treatment, or transient response to antiviral therapy (152).…”

Section: Cytomegalic Endothelial Cellsmentioning

confidence: 99%

New Strategies for Prevention and Therapy of Cytomegalovirus Infection and Disease in Solid-Organ Transplant Recipients

2000

View full text Add to dashboard Cite

SUMMARY In the past three decades since the inception of human organ transplantation, cytomegalovirus (CMV) has gained increasing clinical import because it is a common pathogen in the immunocompromised transplant recipient. Patients may suffer from severe manifestations of this infection along with the threat of potential fatality. Additionally, the dynamic evolution of immunosuppressive and antiviral agents has brought forth changes in the natural history of CMV infection and disease. Transplant physicians now face the daunting task of recognizing and managing the changing spectrum of CMV infection and its consequences in the organ recipient. For the microbiology laboratory, the emphasis has been geared toward the development of more sophisticated detection assays, including methods to detect emerging antiviral resistance. The discovery of novel antiviral chemotherapy is an important theme of clinical research. Investigations have also focused on preventative measures for CMV disease in the solid-organ transplant population. In all, while much has been achieved in the overall management of CMV infection, the current understanding of CMV pathogenesis and therapy still leaves much to be learned before success can be claimed.

show abstract

“…The incidence of infection can vary but in general 50-90% of the population is infected. In the healthy host, the acute phase of infection is self-limiting and the virus establishes a latent infection that can be reactivated in response to a variety of cellular stresses [5]. The long-term effects of viral persistence are the focus of many studies as HCMV infection has been implicated as a co-factor in the development of cardiovascular disease.…”

Section: Introductionmentioning

confidence: 99%

Lipid Metabolism in Vascular Smooth Muscle Cells Infuenced by HCMV Infection

Li¹,

Dai

et al. 2016

Cell Physiol Biochem

View full text Add to dashboard Cite

Background: The present study was designed to observe the infection of human cytomegalovirus (HCMV) to human vascular smooth muscle cells (VSMCs), and the effect of viral infection on lipid metabolism in VSMCs. Methods: The cytopathic effects were observed by inverted microscopy and viral infection were examined by electron microscopy and RT-PCR. The lipid metabolism related gene profiling of VSMCs after HCMV infection was assayed by cDNA assay and the abnormal expression of genes were validated by quantitative RT-PCR. The content of cholesterol in VSMCs after HCMV infection was assayed by cholesterol detection kit. Results: VSMCs showed obvious cytopathic effects after HCMV infection. Intact viral particles could be detected in VSMCs using electron microscope. By use of RT-PCR technology, IE gene of HCMV could be amplified from VSMCs. The expression of cell lipid metabolism related gene profiling showed obvious disorders. The expression levels of HMG-CoA synthase and HMG-CoA reductase after infection increased significantly. The cellular cholesterol content (μmol/10 6 cells) was significantly higher than that of mock infected group at 72h post infection. Conclusion: HCMV can infect VSMCs and the infection can affect cellular lipid metabolism related gene expression, which get involved in the occurrence and development of atherosclerosis (AS).

show abstract

A Sensitive Method for Quantifying Cytomegalic Endothelial Cells in Peripheral Blood from Cytomegalovirus-Infected Patients

Cited by 9 publications

References 33 publications

Applications of Flow Cytometry to Clinical Microbiology

Applications of Flow Cytometry to Clinical Microbiology

New Strategies for Prevention and Therapy of Cytomegalovirus Infection and Disease in Solid-Organ Transplant Recipients

Lipid Metabolism in Vascular Smooth Muscle Cells Infuenced by HCMV Infection

Contact Info

Product

Resources

About