A sensitive, label-free electrochemical detection of telomerase activity without modification or immobilization

Liu, Xu; Wei, Min; Xu, Ensheng; Yang, Haitang; Wei, Wei; Zhang, Hongjie; Liu, Songqin

doi:10.1016/j.bios.2016.12.054

Cited by 39 publications

(13 citation statements)

References 50 publications

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“…Notably, obviously different fluorescence signals were given by one HeLa cell and heat-inactivated control, indicating that the proposed method performs well in telomerase activity detection at the single-cell level. Such high sensitivity was comparable with previously reported methods (Table S2), [13][14][15]20,24,28,44,45 and was the best in the reported labelfree ones. Correspondingly, traditional RCA reaction without Nb.BbvCI addition could only detect telomerase activity in more than 100 HeLa cells (Figure S8), thus supporting the greatly increased signal amplification capacity of nicking enzyme-mediated exponential RCA.…”

Section: ■ Results and Discussionsupporting

confidence: 90%

Label-Free Telomerase Detection in Single Cell Using a Five-Base Telomerase Product-Triggered Exponential Rolling Circle Amplification Strategy

Cui

et al. 2019

ACS Sens.

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Telomerase is a universal biomarker of malignant tumors. Sensitive and reliable analysis for telomerase activity is of vital importance for both early diagnosis and therapy of malignant tumors. Herein, a novel fluorescent strategy was proposed for sensitive and label-free detection of telomerase activity. One highlight of this strategy is that an exponential signal amplification can be triggered by a very short telomerase extension product (TEP). Without adding dATP, the designed telomerase primer can be easily controlled to extend five bases (GGGTT) to give short TEP with definite length. The resulting short TEP can then be constructed as a circular rolling circle amplification (RCA) template and thus initiate a nicking enzyme-mediated exponential RCA, producing G-rich amplification products that can be sensitively probed via specific binding between the fluorescent dye Thioflavin T (ThT) and the nucleic acid G-quadruplexes. Elevated telomerase translocation efficiency, combined with exponential signal amplification and specific probing of RCA products by ThT, endow the sensing platform with extraordinarily high detection sensitivity. The requirement for short TEP increases the possibility to analyze telomerase with low activity. The proposed sensing platform can achieve sensitive telomerase activity detection in individual cells, even with the interference of accumulated normal cells. It was also demonstrated to show excellent capability in screening for the inhibitors of telomerase. Therefore, the proposed sensing platform has great potential for not only clinical diagnosis but also anticancer drug development.

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Section: ■ Results and Discussionsupporting

confidence: 90%

Label-Free Telomerase Detection in Single Cell Using a Five-Base Telomerase Product-Triggered Exponential Rolling Circle Amplification Strategy

Cui

et al. 2019

ACS Sens.

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“…This method requires surface modifications at neither the electrode surface nor the target molecule, and is very fast and efficient. The detection limit of this method is up to 3 cancerous cells (23).…”

Section: Electrochemical Sensorsmentioning

confidence: 96%

Nano-biosensors in cellular and molecular biology

Moradi

Khaledian

Abdoli

et al. 2018

Cell Mol Biol (Noisy-le-grand)

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Detection and quantification of various biological and non-biological species today is one of the most important pillars of all experimental sciences, especially sciences related to human health. This may apply to a chemical in the factory wastewater or to identify a cancer cell in a person's body, it may be apply to trace a useful industrial microorganism or human or plant pathogenic microorganisms. In this regard, scientists from various sciences have always striven to design and provide tools and techniques for identifying and quantifying as accurately as possible to trace various analyte types with greater precision and specificity. Nano science, which has flourished in recent years and is nowadays widely used in all fields of science, also has a unique place in the design and manufacture of sensors and this, in addition to the new and special characteristics of nanoparticles, is due to the ability of nano-devices to penetrate into very tiny places to track the species. On the other hand, due to the high specificity of biological molecules in identifying and connecting to their receptors that have evolved over millions of years, Scientists are now trying to design hybrid devices using nano science and biology, called Nano-biosensors So that they can trace and quantify target molecules in very small amounts and in inaccessible places, such as within the organs and even the cells.

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“…The sensor was validated its performance by changing colour from light blue to dark blue as telomerase activity increased due to chromogenic reaction. To detect telomerase activity, not only the optical biosensor but also the electrochemical biosensor can be applied with good reproducibility and selectivity [28]. The electrochemical biosensor was realized using methylene blue (MB) as a G-quadruplex binding on indium tin oxide.…”

Section: Biosensors For Detection Of Bladder Cancers Using Urinementioning

confidence: 99%

Development of Non-Invasive Biosensor Devices for the Detection of Bladder Cancer in Urine

Park¹,

Kim²,

Jung³

2020

COR

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Bladder cancer (BC) is the fourth most common malignant tumor in the United States. It is the second most common cancer of the urinary system, accounting for 7% of all new cancer cases. It is also the fifth deadliest cancer, accounting for 4% of all cancer-related deaths in the United States. Our efforts to reduce costs of BC diagnosis and improve patients’ quality of life by avoiding unnecessary invasive diagnostic tests resulted in findings of promising urinary biomarkers for the detection of BC. This short review article aims to provide the current status of non-invasive biosensor device development for detection of BC, in particular, in patients’ urine samples.

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A sensitive, label-free electrochemical detection of telomerase activity without modification or immobilization

Cited by 39 publications

References 50 publications

Label-Free Telomerase Detection in Single Cell Using a Five-Base Telomerase Product-Triggered Exponential Rolling Circle Amplification Strategy

Label-Free Telomerase Detection in Single Cell Using a Five-Base Telomerase Product-Triggered Exponential Rolling Circle Amplification Strategy

Nano-biosensors in cellular and molecular biology

Development of Non-Invasive Biosensor Devices for the Detection of Bladder Cancer in Urine

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