A sensitive fluorometric DNA nanobiosensor based on a new fluorophore for tumor suppressor gene detection

Darestani-Farahani, Maryam; Faridbod, Farnoush; Ganjali, Mohammad Reza

doi:10.1016/j.talanta.2018.07.042

Cited by 13 publications

(6 citation statements)

References 31 publications

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“…29–31 The intermediate salt III was reacted with hydrazine hydrate to obtain the cyclized triazole 4-amino-3-(1 H -indol-3-yl)-1 H -1,2,4-triazole-5(4 H )-thione IV , followed by the condensation of the amino group with different aromatic aldehydes in the presence of glacial acetic acid as a catalyst to afford the 4-(benzylideneamino)-5-(1 H -indol-3-yl)-4 H -1,2,4-triazole-3-thiol derivatives Va–n . 28–35 The structures of all the derivatives were confirmed based on the investigation of their analytical and spectral data. All series showed IR signals at 3390–3201 cm −1 for the NH groups in addition to signals at 3097–3001 cm −1 for the aromatic CH groups and signals at 1658–1573 cm −1 for the CN moiety.…”

Section: Resultsmentioning

confidence: 92%

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Novel indolyl 1,2,4-triazole derivatives as potential anti-proliferative agents: in silico studies, synthesis, and biological evaluation

Ghobish,

Mohamed,

Farag

et al. 2024

RSC Med. Chem.

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Section: Resultsmentioning

confidence: 92%

“…Compounds II, III and IV were prepared according to previously reported methods. [28][29][30][31][32][33][34][35] 4.2.2. Preparation of 1H-indole-3-carbohydrazide (II).…”

Section: Rsc Medicinal Chemistry Research Articlementioning

confidence: 99%

Novel indolyl 1,2,4-triazole derivatives as potential anti-proliferative agents: in silico studies, synthesis, and biological evaluation

Ghobish,

Mohamed,

Farag

et al. 2024

RSC Med. Chem.

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“…47 In the other study, a fluorometric method for DNA methylation detection was applied with detection limit of 13 PM. 48 Also, a fluorometric-based nanobiosensor was designed to evaluate DNA methylation with a detection limit of 73 PM. 49 Interestingly, a labeled electrochemical method was used to distinguish DNA methylation with a detection limit of 1 fM.…”

Section: Analytical Studymentioning

confidence: 99%

An innovative fluorometric bioanalysis strategy towards recognition of DNA methylation using opto‐active polymer: A new platform for DNA damage studies by genosensor technology

Aghaei

Hasanzadeh

Dehghan

et al. 2022

J of Molecular Recognition

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Efficient pharmacotherapy of cancer is related to accurate recognition of genetic mutations and epigenetic alterations in the early‐stage diagnosis. In the present study, a novel optical genosensor based on toluidine blue as photonic probe was developed to detection of DNA methylation using hybridization of pDNA with cDNA. Biomedical analysis was performed using UV‐vis and fluorometric methods. For the first time, this strategy was applied for the distinction of methylated DNA from unmethylated‐DNA‐based on the interaction of optical probe with methylated‐DNA and unmethylated DNA. Fluorescence spectroscopic data showed that poly‐toluidine blue could be bind to DNA sequences and lead to different fluorescence patterns and could be used as an efficient geno‐platform for the sensitive bioassay of mutation. The excitation and emission wavelengths were 580 and 630 nm, respectively. Non‐binding of mismatch sequences with the optical probe was used as negative control. Under optimal conditions, linear range was 1 zM to 0.2 pm and the lower limit of quantitation was obtained as target concentrations ranging 1 zM. The designed genosensor showed high capability to distinct methylation from un‐methylated. Therefore, the designed DNA‐based bioassay could detect DNA methylation significantly. Finally, bioanalysis of real samples showed that the designed genosensor could use to detect DNA methylation which is a new platform for point of care analysis.

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“…Indole-triazole-3-thione heterocyclic systems have gained a lot of attention in the research community due to their significant applications in different fields like pharmaceutical, agrochemical, coordination chemistry and biosensor applications [10,11]. Among the biological applications, these indole-triazole-3-thione hybrids have been shown to have strong inhibition activities against many enzymes such as AChE [12], TNF-α [13], (ADAMTS-5) [14], dizinc metallo-β-lactamase [15], and ureases inhibition [16].…”

Section: Introductionmentioning

confidence: 99%

X-ray Single Crystal Structure, Tautomerism Aspect, DFT, NBO, and Hirshfeld Surface Analysis of a New Schiff Bases Based on 4-Amino-5-Indol-2-yl-1,2,4-Triazole-3-Thione Hybrid

et al. 2021

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Four different new Schiff basses tethered indolyl-triazole-3-thione hybrid were designed and synthesized. X-ray single crystal structure, tautomerism, DFT, NBO and Hirshfeld analysis were explored. X-ray crystallographic investigations with the aid of Hirshfeld calculations were used to analyze the molecular packing of the studied systems. The H···H, H···C, S···H, Br···C, O···H, C···C and N···H interactions are the most important in the molecular packing of 3. In case of 4, the S···H, N···H, S···C and C···C contacts are the most significant. The results obtained from the DFT calculations indicated that the thione tautomer is energetically lower than the thiol one by 13.9545 and 13.7464 kcal/mol for 3 and 4, respectively. Hence, the thione tautomer is the most stable one which agree with the reported X-ray structure. In addition, DFT calculations were used to compute the electronic properties while natural bond orbital calculations were used to predict the stabilization energies due to conjugation effects. Both compounds are polar where 4 (3.348 Debye) has a higher dipole moment than 3 (2.430 Debye).

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A sensitive fluorometric DNA nanobiosensor based on a new fluorophore for tumor suppressor gene detection

Cited by 13 publications

References 31 publications

Novel indolyl 1,2,4-triazole derivatives as potential anti-proliferative agents: in silico studies, synthesis, and biological evaluation

Novel indolyl 1,2,4-triazole derivatives as potential anti-proliferative agents: in silico studies, synthesis, and biological evaluation

An innovative fluorometric bioanalysis strategy towards recognition of DNA methylation using opto‐active polymer: A new platform for DNA damage studies by genosensor technology

X-ray Single Crystal Structure, Tautomerism Aspect, DFT, NBO, and Hirshfeld Surface Analysis of a New Schiff Bases Based on 4-Amino-5-Indol-2-yl-1,2,4-Triazole-3-Thione Hybrid

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