A sensitive EZMTT method provides microscale, quantitative and high-throughput evaluation of drug efficacy in the treatment of Mycobacterium tuberculosis infectious diseases

Xie, Li; Zhao, Chen; Liu, Wei; Gu, Dongshi; Yu, Yan; Chen, Xinrou; Wu, Yezhou; Xie, Jinlu; Zhao, Gang; Ruan, Benfang

doi:10.1016/j.mimet.2021.106136

Cited by 3 publications

(1 citation statement)

References 27 publications

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“…This causes the use of many different specific and non-specific growth media, such as general-purpose nutrient agar (Smułek et al, 2020 [ 2 ]) and enrichment broth (Rahman et al, 2020 [ 3 ]), specific for certain fungi Sabouraud’s agar (Cultrera et al, 2021 [ 4 ]), and Middlebrook and Löwenstein-Jensen media used for Mycobacterium spp. culture (Schön et al, 2020 [ 5 ]; Xie et al, 2021 [ 6 ]) (Mikolajczyk et al, 2016 [ 7 ]).…”

Section: Introductionmentioning

confidence: 99%

New Biocomposite Electrospun Fiber/Alginate Hydrogel for Probiotic Bacteria Immobilization

et al. 2021

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Biotechnological use of probiotic microorganisms involves providing them with appropriate conditions for growth, but also protection against environmental changes caused by an exchange of the medium, isolation of metabolites, etc. Therefore, the research on effective immobilization of probiotic microorganisms should be focused in this direction. The present study aimed to evaluate the effectiveness of an innovative hybrid immobilization system based on electrospun nanofibers and alginate hydrogel. The analyses carried out included the study of properties of the initial components, the evaluation of the degree and durability of cell immobilization in the final material, and their survival under stress conditions. Effective binding of microorganisms to the hydrogel and nanofibers was confirmed, and the collected results proved that the proposed biocomposite is an efficient method of cell protection. In addition, it was shown that immobilization on electrospun nanofibers leads to the preservation of the highest cell activity and the least cell growth restriction as compared to free or lyophilized cells only. The completed research opens new perspectives for the effective immobilization of microorganisms of significant economic importance.

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Section: Introductionmentioning

confidence: 99%

New Biocomposite Electrospun Fiber/Alginate Hydrogel for Probiotic Bacteria Immobilization

et al. 2021

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Automated antimicrobial susceptibility testing of slow-growing Pseudomonas aeruginosa strains in the presence of tetrazolium salt WST-1

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Chalansonnet

et al. 2021

Journal of Microbiological Methods

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Recent Advances in Rapid Antimicrobial Susceptibility Testing

et al. 2021

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Background Antimicrobial susceptibility testing (AST) is classically performed using growth-based techniques that essentially require viable bacterial matter to become visible to the naked eye or a sophisticated densitometer. Content Technologies based on the measurement of bacterial density in suspension have evolved marginally in accuracy and rapidity over the 20th century, but assays expanded for new combinations of bacteria and antimicrobials have been automated, and made amenable to high-throughput turn-around. Over the past 25 years, elevated AST rapidity has been provided by nucleic acid-mediated amplification technologies, proteomic and other “omic” methodologies, and the use of next-generation sequencing. In rare cases, AST at the level of single-cell visualization was developed. This has not yet led to major changes in routine high-throughput clinical microbiological detection of antimicrobial resistance. Summary We here present a review of the new generation of methods and describe what is still urgently needed for their implementation in day-to-day management of the treatment of infectious diseases.

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A sensitive EZMTT method provides microscale, quantitative and high-throughput evaluation of drug efficacy in the treatment of Mycobacterium tuberculosis infectious diseases

Cited by 3 publications

References 27 publications

New Biocomposite Electrospun Fiber/Alginate Hydrogel for Probiotic Bacteria Immobilization

New Biocomposite Electrospun Fiber/Alginate Hydrogel for Probiotic Bacteria Immobilization

Automated antimicrobial susceptibility testing of slow-growing Pseudomonas aeruginosa strains in the presence of tetrazolium salt WST-1

Recent Advances in Rapid Antimicrobial Susceptibility Testing

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