A sensitive electrochemical assay for T4 polynucleotide kinase activity based on titanium dioxide nanotubes and a rolling circle amplification strategy

Abstract

An ultrasensitive electrochemical biosensor was developed for detection of T4 polynucleotide kinase activity based on titanium dioxide nanotubes and a rolling circle amplification strategy.

An electrochemical biosensor for T4 polynucleotide kinase activity assay based on host–guest recognition between phosphate pillar[5]arene@MWCNTs and thionine

“…Next, phosphorylation of the substrate DNA on the electrode surface was carried out in the presence of T4 PNK and ATP, which could introduce a phosphorylation site at the 5′-OH termini of the substrate DNA. Then, TiO 2 nanoparticles were attached to the phosphorylated substrate DNA (P-DNA) by the specific affinity between Ti 4+ and phosphate groups, 22,26 leading to the formation of TiO 2 /P-DNA/Au for anchoring PP5@MWCNTs. Herein, Ti 4+ acts as a bridge to connect the phosphorylated substrate DNA and PP5@MWCNTs with phosphate groups via robust phosphate-Ti 4+ -phosphate chemistry.…”

“…Alternatively, a variety of new approaches have been developed for PNK testing, including colorimetric, 14,15 fluorescent, 16,17 electrochemiluminescent, 18,19 photoelectrochemical, 20,21 and electrochemical methods. [22][23][24][25][26][27] Among these methods, electrochemical approaches are gaining popularity because of their high sensitivity, cheap cost, potential for downsizing, and quick reaction time compared to those of other options. Due to their good biocompatibility, unique structures, and excellent optical and electric features, different nanomaterials and nanocomposites, such as semiconductor nanomaterials, MXenes, metalorganic frameworks (MOFs), quantum dots (QDs), and metal nanoclusters and nanoparticles are being introduced into electrochemical sensors to further improve their detection performance.…”

“…Due to their good biocompatibility, unique structures, and excellent optical and electric features, different nanomaterials and nanocomposites, such as semiconductor nanomaterials, MXenes, metalorganic frameworks (MOFs), quantum dots (QDs), and metal nanoclusters and nanoparticles are being introduced into electrochemical sensors to further improve their detection performance. [24][25][26][27][28][29] The electrochemical technique has made enormous strides in the measurement of T4 PNK activities and inhibitor screening, but developing more straightforward, fast, and sensitive modalities for T4 PNK activity evaluation remains a significant issue.…”

A Sensitive Electrochemical Assay for T4 Polynucleotide Kinase Activity Based on Fe3O4@TiO2 And Gold Nanoparticles Hybrid Probe Modified Magnetic Electrode

“…[26][27][28][29][30][31][32] To improve sensitivity, various nanomaterials are introduced in electrochemical sensors due to the their excellent optical and electric properties, unique structures, and good biocompatibility, such as metal nanoparticles and nanoclusters, carbon nanomaterials, quantum dots (QDs), metal−organic frameworks (MOFs), MXenes, semiconductors nanomaterials, and their nanocomposites. [32][33][34][35][36][37][38][39][40][41][42][43][44] Although great achievements of these electrochemical approaches have been developed for the detection of T4 PNK activity and screening its inhibitors, it still remains a challenge to develop more simple, rapid, accurate and sensitive methods for T4 PNK activity assay.…”

“…Based on the fact that TiO 2 is used as effective identifier or capture material to selectively bind the phosphate functional groups of phosphorylated peptides, various biosensors were fabricated for the detection of T4 PNK. 26,33,36,45 Recently, Yan et al proposed a titanium-based metalorganic framework (Ti-MIL125-NH 2 ) as a novel enrichment platform for detecting protein kinase A activity by advantage of the highly specific recognition of phosphate groups by the titanium sites of Ti-MIL125-NH 2 nanoparticle. 46 Through the appropriate signal amplification strategy, the improvement of sensitivity and analytical performance for T4 PNK activity assay should be reasonably conceivable.…”