A sensitive detection assay based on signal amplification technology for Alzheimer's disease's early biomarker in exosome

Zhou, Jie; Meng, Lingchang; Ye, Weiran; Wang, Qiaolei; Geng, Shizhen; Sun, Chong

doi:10.1016/j.aca.2018.03.016

Cited by 43 publications

(13 citation statements)

References 41 publications

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“…However, these methods need high-cost equipment, complicated sample processing and long runtime, and are therefore not suitable for high-throughput routine clinical trials and scientific research applications [22][23][24]. Because of their low detection limit and high selectivity, fluorescent probes have been used for the quantitative detection of biothiols [25][26][27][28]. Cho's group [29] reported a two-photon fluorescent probe for the detection of thiols based on a thiol/disulfide exchange.…”

Section: Introductionmentioning

confidence: 99%

A novel and simple imidazo[1,2-a]pyridin fluorescent probe for the sensitive and selective imaging of cysteine in living cells and zebrafish

Zhu

Wang

et al. 2019

Analytica Chimica Acta

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Cysteine (Cys), homocysteine (Hcy) and glutathione (GSH) play many crucial physiological roles in organisms. Their abnormal levels can cause and indicate various diseases. In the present study, a small-molecule fluorescent probe 2-(imidazo[1,2-a]pyridin-2-yl)phenyl acrylate (IPPA) was designed, synthesized and characterized by NMR, FT-IR and HRMS. IPPA can selectively detect Cys over other analytes because of an approximately 76 times enhancement in fluorescence intensity. The limit of detection of IPPA for Cys was 0.33 μM. The pseudo-first-order rate constant of the reaction between IPPA and Cys was approximately 10 times that of the reaction between IPPA and Hcy (K Cys 3.18 × 10 −3 S −1 vs K Hcy 4.92 × 10 −4 S −1), indicating that Cys can be distinguished from Hcy. In addition, IPPA exhibits strong anti-interference ability, small molecular weight, high efficiency, low toxicity and good cell permeability. It was successfully used in imaging HepG2 cells and zebrafish. The fluorescence response of IPPA for calf serum are powerful proofs for practical application. Therefore, IPPA has high potential for bioassay applications.

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Section: Introductionmentioning

confidence: 99%

A novel and simple imidazo[1,2-a]pyridin fluorescent probe for the sensitive and selective imaging of cysteine in living cells and zebrafish

Zhu

Wang

et al. 2019

Analytica Chimica Acta

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“…The latter will be emphasized in this Commentary. A notable example of the former role is detection in blood of exosomes that contain Alzheimer disease-specific oligomeric proteins and thereby providing the potential to provide ultrasensitive early detection of Alzheimer disease [ 25 ]. Concerning the latter role, increasing evidence indicates that exosomes from MSCs mediate the beneficial effects of stem cell therapy in various pathological neurological states, including perinatal brain injury (see later).…”

Section: Exosomesmentioning

confidence: 99%

Commentary – Exosomes: Realization of the great therapeutic potential of stem cells

Volpe

2020

NPM

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“…Considering the above, the use of exosome-associated Aβ oligomers for early detection of Alzheimer's disease has been trending and along with other targets is the subject of patents. A contemporarily declared technology used aptasensors for detection of picomolar concentrations of Aβ42 in blood of suspected AD subjects [79]. It is rationalized that the utility of the exosomes within the developed strategy stems from their ability to cross the blood brain barrier.…”

Section: Exosomes In Neurological Disordersmentioning

confidence: 99%

Exosomes: Biological Couriers with Transformative Messages

Yousef¹,

Abdelnaser²

2019

J. Biomed

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Exosomes are nanovesicles produced by almost all cell types to the extracellular environment. These structures, initially mistaken for "cellular dust", are key effectors of cell-cell communication and play major roles in maintaining normal physiological function and homeostatic balance as well as relaying pathology. Given their non-immunogenicity, their native membrane composition combined with the wealth of contained bioinfomative cargoes and coupled to their innate ability to cross multiple biological barriers, these tiny sacs have merited a great deal of interest in recent years and are slowly edging their way to the surface. Accruing evidence corroborating the far-reaching potential of exosomes is ever growing and easily demonstrable. Nonetheless, progress with translation of the reported outcomes to clinical settings and recognition of these tools as a multi-application platform remains much too inadequate for the outstanding potential of these biological messengers and the entailed shift in theragnostic paradigms. The purpose of this review is to recapitulate on the most recent advances in exosome research and accentuate their momentous promise as game changers in disease diagnostics and therapeutics. Finally, market transition and commercialization efforts are highlighted with a brief commentary on the status quo. Syndecans/syntenin system: Syndecans are membrane-associated proteoglycans, whereas syntenin is a syndecan-interacting protein. Syntenin contains a PDZ domain, which constitutes the site of interaction with syndecan. The syndecan/syntenin system has been proven to play a key role in exosomal biogenesis [98]. 4Cortactin: a protein involved in cytoskeletal modification.

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A sensitive detection assay based on signal amplification technology for Alzheimer's disease's early biomarker in exosome

Cited by 43 publications

References 41 publications

A novel and simple imidazo[1,2-a]pyridin fluorescent probe for the sensitive and selective imaging of cysteine in living cells and zebrafish

A novel and simple imidazo[1,2-a]pyridin fluorescent probe for the sensitive and selective imaging of cysteine in living cells and zebrafish

Commentary – Exosomes: Realization of the great therapeutic potential of stem cells

Exosomes: Biological Couriers with Transformative Messages

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