A sensitive bithiophene-based biosensor for interferon-gamma characterization and analysis

Tsai, Pei-I; Lee, S.S.; Chou, Shin-Ting; Jan, C.M.; Chang, Yeong-Sheng; Lee, A.S.Y.; Lee, C.K.

doi:10.1016/j.tsf.2013.12.037

Cited by 4 publications

(5 citation statements)

References 23 publications

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“…The thiophene-based gold chips were rinsed thoroughly with THF, ethanol and distilled water and then dried with nitrogen before being assembled onto the holder. An ellipsometry instrument built by our team was used to measure the physical characteristics of the bithiophene biolinker on a home-made self-coated Au chip [ 24 ]. A total of 10 measurement points were taken to obtain the mean values and standard deviation of the bithiophene biolinker, i.e., refraction index (RI) was 1.5355 ± 0.2567 and thickness was 3.0111 ± 0.6964 nm.…”

Section: Methodsmentioning

confidence: 99%

“…For rapid screening simulation of an antisera antibody experiment, we synthesized a 1.23 nm bithiophene-based conductive biolinker [ 24 ] to enhance and verify the anti-IFN-γ antibody interaction detection capability of our system. In our study, the bithiophene linker was employed to examine the thin film surface coating and its specific binding ability of a sensor surface.…”

Section: Introductionmentioning

confidence: 99%

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Enhanced Signal and Quantitative Detection of Anti-Interferon-Gamma Antibody by Using a Nanometer Biolinker

Tsai

Lee

et al. 2016

PLoS ONE

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For rapid screening and quantification of an antisera antibody, a nanometer bithiophene-based conductive biolinker can enhanced signal performance and can be used to verify the interaction of an anti-IFN-γ antibody with an IFN-γ protein. The experimental measurements take a generic approach which takes advantage of the functionality of thiophene-based linkers for biosensors. Effects associated with using bithiophene as a biolinker for surface plasmon resonance (SPR) spectroscopy are examined in this paper. By using an atomic force microscope (AFM), it was observed that the morphology of the bithiophene modified gold sensor surface became smoother than the original gold surface. We compared the response and concentration of the anti-IFN-γ antibody on a bithiophene-coated and dextran-coated biochip as well as on different thickness-modified surfaces under SPR relevant conditions. The results indicate that a response to IFN-γ molecules immobilized on a sensor using a bithiophene biolinker improved more than 8-fold when compared to that of a sensor using a dextran biolinker. Furthermore, the regeneration ability of the sensor surface shows good repeatability as only less than a 1% decrease was found after repeating the experimental work over 6 cycles. The characteristics provided us with a good platform for rapid screening, real-time monitoring and quantitative concentration of the autoimmune antibody activities.

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Enhanced Signal and Quantitative Detection of Anti-Interferon-Gamma Antibody by Using a Nanometer Biolinker

Tsai

Lee

et al. 2016

PLoS ONE

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“…The semi-conductive linker CS20S (5'-(mercapto)-[2, 2'-bithiophene]-5-carboxylic acid) was dissolved in tetrahydrofuran (THF) that was synthesized within the team [12]. The anti-human IFN-γ Ab, recombinant human IFN-γ, and monoclonal mouse IgG were obtained from R&D Systems (Minneapolis, MN, USA).…”

Section: Materials and Self-assembled Monolayermentioning

confidence: 99%

Integrating SPR-ellipsometry and electrochemical measurements for performance evaluation of label-free thiophene-based biosensor

Tsai

Lee

Chou

et al. 2014

Frontiers in Biological Detection: From Nanosensors to Systems VI

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The surface plasmon resonance reflectance changes measured with a circularly polarized ellipsometry and an electrochemical impedance spectroscopy were identified to be able to characterize the critical roles of biomolecules for vastly different biological functions and processes. Throughout the course of this study, interferon-gamma (IFN-γ) was chosen as the biomarker to test and to verify the performance of this newly developed system for Tuberculosis detection. The interactions of IFN-γ with immobilized anti-IFN-γ antibody at various concentrations were interrogated both optically and electrochemically. A semi-conductive linker bis-thiophene was thiolated to ensure the cross-linked monoclonal human IFN-γ antibody got self-assembled onto the gold thin film and form a label-free biosensor. The functional features of the bis-thiophene coated-gold film were characterized by cyclic voltammetry and impedance spectroscopy methods. The association of IFN-γ to the bis-thiophene bridging units via antibody-antigen interactions provided the basis for ultrasensitive detection of IFN-γ by tracking the conformation changes in surface-bound protein molecules. The phase shift can be attributed to the average thickness and the real-time index of refraction of the protein layer in different protein layer. Experimental results obtained by impedance spectroscopy and by phase-interrogation SPR showed linear dynamic range. Our experimental results verified that an increase in the concentration of the IFN-γ usually accompanied by phase increase in SPR and an impedance decrease in EIS. These results indicated that our newly developed integrated biosensing system can potentially provide new insight into various conjugate phenomena and interfacial processes for observing molecular conformation changes.

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“…As far as examples of application in medicine and pharmaceutical science are concerned, 5-(4-hydroxyphenyl)-5'-dicyanoethenyl-2,2'-bithiophene was tested as a marker of cell lesions in Alzheimer’s disease [8]. Moreover, 5'-mercapto-2,2'-bithiophene-5-carboxylic acid was tested as a potential biosensor detecting antigen-antibody connection [9], and a series of 5-( E )-styryl-2,2'-bithiophenes were tested as potential β-amyloid probes [10]. The starting substances for the synthesis of these compounds are simple derivatives of thiophene, bithiophene or oligothiophene substituted by Li, Br, I, C≡CH, MgCl, SnBu 3 , B(OH) 2 , B(OR) 2 , or ZnCl, which facilitate the chemical modification of their structures.…”

Section: Introductionmentioning

confidence: 99%

Multifaceted Strategy for the Synthesis of Diverse 2,2'-Bithiophene Derivatives

et al. 2015

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New catalytically or high pressure activated reactions and routes, including coupling, double bond migration in allylic systems, and various types of cycloaddition and dihydroamination have been used for the synthesis of novel bithiophene derivatives. Thanks to the abovementioned reactions and routes combined with non-catalytic ones, new OPEN ACCESSMolecules 2015, 20 4566 acetylene, butadiyne, isoxazole, 1,2,3-triazole, pyrrole, benzene, and fluoranthene derivatives with one, two or six bithiophenyl moieties have been obtained. Basic sources of crucial substrates which include bithiophene motif for catalytic reactions were 2,2'-bithiophene, gaseous acetylene and 1,3-butadiyne.

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A sensitive bithiophene-based biosensor for interferon-gamma characterization and analysis

Cited by 4 publications

References 23 publications

Enhanced Signal and Quantitative Detection of Anti-Interferon-Gamma Antibody by Using a Nanometer Biolinker

Enhanced Signal and Quantitative Detection of Anti-Interferon-Gamma Antibody by Using a Nanometer Biolinker

Integrating SPR-ellipsometry and electrochemical measurements for performance evaluation of label-free thiophene-based biosensor

Multifaceted Strategy for the Synthesis of Diverse 2,2'-Bithiophene Derivatives

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