A sensitive assay for measuring aspartate-specific amino acid racemase activity

Racemases and epimerases operating through a 1,1-proton transfer mechanism: reactivity, mechanism and inhibition

“…This can be achieved using an irreversible coupled enzyme to remove the reaction product. 107,159,160 There are a number of examples of the use of coupling enzymes in kinetic studies determining K m and k cat values (Table 1). The most common coupling enzymes used are D-amino acid oxidase and NAD-dependent oxidoreductases.…”