A Sensitive and Wide Coverage Ambient Mass Spectrometry Imaging Method for Functional Metabolites Based Molecular Histology

Abstract

Figure 2. A) MS images of representative metabolites in tissue homogenate model under different spray solvent systems. B) Relative ion intensity of endogenous ions (m/z 70-1000) with no extracting air flow (NER) or with high-rate extracting air flow (HER).

DESI-MSI and METASPACE indicates lipid abnormalities and altered mitochondrial membrane components in diabetic renal proximal tubules

“…Further, another advantage of DESI is its capability for highthroughput analysis as minimal sample preparation is needed, where, for example, coating samples with matrices is not required. DESI-MSI-based spatially resolved metabolomics in different cancer studies have provided new insights into the understanding of tumorassociated metabolic reprogramming and provided a novel approach for functional metabolites based molecular histology (He et al 2018;Huang et al 2019;Sun et al 2019). In addition, DESI-MSI has previously been employed to analyze kidney tissues and other tissue samples (Eberlin et al 2013(Eberlin et al , 2014Wiseman et al 2008;Zhang et al 2017) although diabetic changes in mouse kidneys have not previously reported.…”

Spatially resolved metabolomics to discover tumor-associated metabolic alterations

“…Untargeted AMSI has been regarded as a rational approach for monitoring lipid dysregulation in cancer tissues for biological and clinical studies (21–25). Airflow-assisted desorption electrospray ionization (AFADESI)-MSI is a high-coverage ambient molecular imaging technique that was developed by our group, and it can map numerous functional metabolites located in different metabolic pathways (26). However, the chemical noise arising from tissue biomatrix makes the directly mapping of proteins using AMSI techniques challenging, especially for the low-content functional metabolic enzymes.…”

Fabrication of homogenous three‐dimensional biomimetic tissue for mass spectrometry imaging

“…AFADESI was employed to perform the ambient MSI analysis of the tissue sections. The general setup was established as previously reported . The commercial ion source was modified in‐house with a stainless‐steel transport tube (inner diameter 3 mm, outer diameter 4 mm, length 500 mm) and an extracting tube connected to a vacuum pump (MZ2C NT, Vacuubrand, Germany).…”

“…The general setup was established as previously reported. 38,39 The commercial ion source was modified in-house with a stainless-steel transport tube (inner diameter 3 mm, outer diameter 4 mm, length 500 mm) and an extracting tube connected to a vacuum pump (MZ2C NT, Vacuubrand, Germany). This was coupled to the Orbitrap mass spectrometer (Q Exactive, Thermo Fisher Scientific, San Jose, USA) to acquire the MSI data.…”