A sensitive and validated method for determination of melamine residue in liquid milk by reversed phase high-performance liquid chromatography with solid-phase extraction

Sun, Hanwen; Wang, Lixin; Ai, Lianfeng; Liang, Shan; Hong, Wei

doi:10.1016/j.foodcont.2009.10.008

Cited by 140 publications

(73 citation statements)

References 7 publications

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“…Various techniques including HPLC-UV [10][11][12] or hydrophilic interaction liquid chromatography (HILIC)-UV [13], HPLC-MS [14][15][16][17][18][19][20][21][22][23][24], GC-MS [24][25][26], desorption ionization MS [27], CE [28][29][30], electrochemical method [31], etc., have been used to detect melamine or cyanuric acid. For the simultaneous determination of melamine and cyanuric acid, HPLC-MS [32] and MALDI-MS [33] were employed.…”

Section: Introductionmentioning

confidence: 99%

Simultaneous determination of melamine and related compounds by hydrophilic interaction liquid chromatography–electrospray mass spectrometry

Xia

Zhou

et al. 2010

J of Separation Science

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Simultaneous determination of melamine and related compounds by hydrophilic interaction liquid chromatographyelectrospray mass spectrometryA hydrophilic interaction liquid chromatography coupled to ESI-MS (HILIC/ESI-MS) method for the simultaneous determination of melamine and related compounds, i.e. ammeline, ammelide and cyanuric acid in foods was developed and validated. The separation was accomplished on a Venusil HILIC column with a mobile phase of acetonitrile 1 10 mM ammonium formate buffer solution at pH 3.5 (88:12, v/v) under isocratic elution mode. For the detection of the targets, the ESI probe worked in the positive and negative switching mode. For each compound, three ions were selected as qualitative ions to obtain high specificity, and the most abundant ion of each compound was selected for quantification to obtain high sensitivity. The target compounds were quantified using SIM with 15N3-melamine and 13C3-cyanuric acid being used as an internal standards in the positive and negative modes, respectively. Compared with RP separation mode, HILIC has merits such as high separation and anti-interference efficiency. The method validation including linearity, LOD, LOQ, precision and recovery proved that the method has merits such high sensitivity, specificity and simplicity versus the other methods reported in the literature.

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Section: Introductionmentioning

confidence: 99%

Simultaneous determination of melamine and related compounds by hydrophilic interaction liquid chromatography–electrospray mass spectrometry

Xia

Zhou

et al. 2010

J of Separation Science

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“…Melamine content if added to the food stuff is associated with several health disorders like urolithiasis, tissue injury, and even bladder cancer (Newton and Utley 1978). Fatality of several infants in China due to the presence of high concentrations of melamine (0.1-2500 μg.mL ) in infant milk products has attracted worldwide attention (Sun et al 2010). The USFDA has set tolerance level of less than 1 mg.L −1 melamine in infant formula and maximum tolerance level of 2.5 mg.L −1 in other foods (Smith et al 2007).…”

Section: Introductionmentioning

confidence: 99%

HPTLC-MS analysis of melamine in milk: standardization and validation

Rani

Medhe

Srivastava

2014

Dairy Sci. & Technol.

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Melamine containing large amount of nitrogen may be deliberately added to milk in order to increase the protein content for economic adulteration in food stuffs. High performance thin layer chromatography-mass spectrometry (HPTLC-MS)-based method of quantification of melamine has been developed using Silica gel 60 F 254s HPTLC plates, with optimized mobile phase iso-propanol/dichloromethane/water, 5:2.5:3, v/v/v in a twin trough chamber saturated (5 min) at pH 6.8. Validation was carried out by testing its specificity, linearity (1-50 μg), accuracy (95.908-98.369%), inter-and intraday precision (1.578 and 1.670%), respectively, limits of detection (2.688 ng) and quantification (8.146 ng). HPTLC in combination with MS has been found suitable for routine analysis of melamine added as adulterant to market-purchased milk samples.

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“…Desta forma, vários procedimentos para a determinação de melamina nas mais diversas matrizes têm sido desenvolvidos, a maioria baseados em cromatografia líquida de alta eficiência [120][121][122][123][124]. Outros procedimentos empregando eletroforese capilar [112,119], cromatografia de íons [125], cromatografia a gás acoplada a espectrometria de massas [113], voltametria [117], espectrometria de massas MALDI-TOF [126], quimiluminescência [127,128], imunoensaios [129] e espectrometrias Ramam [110] e por fluorescência [130] foram desenvolvidos.…”

Section: Determinação De Melamina Em Leiteunclassified

“…Outros procedimentos empregando eletroforese capilar [112,119], cromatografia de íons [125], cromatografia a gás acoplada a espectrometria de massas [113], voltametria [117], espectrometria de massas MALDI-TOF [126], quimiluminescência [127,128], imunoensaios [129] e espectrometrias Ramam [110] e por fluorescência [130] foram desenvolvidos. Estes procedimentos geralmente envolvem preparo de amostra demorado [126], incluindo etapas de extração e pré-concentração e em alguns casos, alto consumo de solventes orgânicos [120][121][122][123][124]. Neste contexto, as técnicas de análises em fluxo apresentam características atrativas, sendo uma opção conveniente para o desenvolvimento de novos procedimentos para a determinação de melamina.…”

Section: Determinação De Melamina Em Leiteunclassified

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Estratégias para aumento de sensibilidade e seletividade de separações cromatográficas em sistemas de análises em fluxo

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A sensitive and validated method for determination of melamine residue in liquid milk by reversed phase high-performance liquid chromatography with solid-phase extraction

Cited by 140 publications

References 7 publications

Simultaneous determination of melamine and related compounds by hydrophilic interaction liquid chromatography–electrospray mass spectrometry

Simultaneous determination of melamine and related compounds by hydrophilic interaction liquid chromatography–electrospray mass spectrometry

HPTLC-MS analysis of melamine in milk: standardization and validation

Estratégias para aumento de sensibilidade e seletividade de separações cromatográficas em sistemas de análises em fluxo

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