A sensitive and specific enzyme-linked immunosorbent assay for the detection of pymetrozine in vegetable, cereal, and meat

Yin, Xiang; Li, Huaming; Wu, Shuangmin; Liu, Yanrong; Yang, Yalin; Qin, Liangni; Li, Long; Xiao, Jiaxu; Liang, Jixiang; Sui, Yu; Le, Tao; Peng, Dapeng

doi:10.1016/j.foodchem.2023.135949

Cited by 21 publications

(7 citation statements)

References 11 publications

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“…Ic-ELISA was used to determine the titer and sensitivity of antibodies to PR in mice serum. Cell fusion and ascites production were based on the method previously described [ 21 ]. Simply, the mice with the serum showing the best titers were euthanized and SP2/0 myeloma cells were mixed with splenocytes in 50% polyethylene glycol 1450 for 1 min, left to stand for 1 min, and terminated by 40 mL of PBS.…”

Section: Methodsmentioning

confidence: 99%

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A Sensitive and Specific Monoclonal Antibody Based Enzyme-Linked Immunosorbent Assay for the Rapid Detection of Pretilachlor in Grains and the Environment

Zhang,

Yin,

Yang

et al. 2023

Foods

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Pretilachlor is a chloroacetamide herbicide mainly used for weed and broadleaf weed control in rice, that is widely utilized in China. In order to detect the residue of pretilachlor in the environment and food, a highly sensitive and specific monoclonal antibody (mAb) against pretilachlor was prepared, and the half maximum inhibitory concentration (IC50) of the monoclonal antibody was validated to be 31.47 ± 2.35 μg/L. An indirect competitive ELISA (ic-ELISA) based on the antibody with a linear range of 6.25~100 μg/L was developed. The specificity of the antibody was explained by computer simulations and experimental validation. The mAb exhibited negligible cross-reactivity towards alachlor, acetochlor, propisochlor, butachlor, and metalaxyl, and the limits of detection (LOD) for pretilachlor in lake, rice, and soil samples were 4.83~5.23 μg/L. The recoveries of all samples were 78.3~91.3%. The reliability of the ic-ELISA method for residue detection of pretilachlor in the environment and grains was confirmed using high performance liquid chromatography.

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Section: Methodsmentioning

confidence: 99%

“…PR was used as a competitor and the ic-ELISA was optimized based on previous studies [ 21 ], with some modifications. To improve the sensitivity of the reaction, the effects of pH and PR dilutions on the ic-ELISA method were investigated.…”

Section: Methodsmentioning

confidence: 99%

A Sensitive and Specific Monoclonal Antibody Based Enzyme-Linked Immunosorbent Assay for the Rapid Detection of Pretilachlor in Grains and the Environment

Zhang,

Yin,

Yang

et al. 2023

Foods

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“…Based on the acetonitrile-optimized standard curve in Section 3.1.4, the ic-ELISA has a minimum detection limit of 1.7 ng/ mL and a linear range of 1.7−73.2 ng/mL in various agricultural samples, thereby meeting the detection limit requirements for the aforementioned samples. Li et al 28 and Yin et al 32 both utilized nitrogen gas drying in the sample preparation and extraction process. Similarly, to address matrix effects, the prepared sample solutions were diluted with PBSTG buffer at a 10-fold dilution followed by ic-ELISA detection.…”

Section: Establishment Of the Standard Curve For Prometryn Colloidal ...mentioning

confidence: 99%

Rapid Detection of Prometryn Residues in Agricultural Samples: Method Comparison of the ic-ELISA and Colloidal Gold Immunochromatographic Test Strips

Gao,

Abd El-Aty,

et al. 2024

ACS Agric. Sci. Technol.

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This study aimed to establish two methods, namely, indirect competitive enzyme-linked immunosorbent assay (ic-ELISA) and colloidal gold immunochromatographic test strips, for detecting prometryn residues in various agricultural samples, such as corn, pumpkin, lotus root, rice, and brown rice. Monoclonal antibodies specific for prometryn were utilized in both methods. The ic-ELISA method demonstrated a maximum half-inhibition concentration (IC 50 ) of 2.4 ng/mL and a lower limit of detection of 0.5 ng/mL. When used in conjunction with a card reader, the colloidal gold immunochromatographic test strip exhibited an IC 50 of 18.6 ng/mL for prometryn detection. The recovery rates of both methods were found to be consistent with those of liquid chromatography tandem mass spectrometry. This consistency indicated the suitability of both methods for detecting prometryn in five different agricultural samples, suggesting that this is a convenient and practical approach for screening prometryn in agricultural products.

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“…A variety of immunoassays are increasingly applied in the detection of veterinary drug residues because they are fast, simple, and cheap. Traditionally, there are three immunoassays, including an enzyme-linked immunosorbent assay (ELISA) [39,40], a lateral flow immunoassay (LFI) [41,42], and a chemiluminescence immunoassay (CLI) [43,44]. For instance, Byzova et al [41] carried out rapid qualitative and quantitative control of the veterinary drug bacitracin in milk based on the lateral flow immunoassay method.…”

Section: Immunoassaymentioning

confidence: 99%

A Review of Veterinary Drug Residue Detection: Recent Advancements, Challenges, and Future Directions

Jun

Wan

2023

Sustainability

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Veterinary drug residues of common food (milk, meat) have posed serious threats to the environment and human health, making the quality and safety of agricultural, livestock, and aquatic products increasingly prominent. With the widespread use of veterinary drugs and the requirements for food safety, it has become urgent to detect veterinary drug residues in animal-derived foods. So far, few studies have systematically reviewed the progresses, challenges, and future directions in veterinary drug residue detection. A thorough review on the current advancements, challenges, and potential future directions of veterinary drug residue detection will be extremely beneficial and timely. This study reviewed recent developments of detection technology of veterinary drug residues. The current issues and challenges in veterinary drug residue detection were examined and highlighted. Finally, future proposals on directions and prospects for veterinary drug residue detection were suggested. High-throughput and high-sensitivity veterinary drug detection technology, sample pretreatment technology for rapid processing, and the fusion of multiple detection methods were recommended as the main directions for the future development of veterinary drug residue detection. It was suggested to develop the analysis and detection technologies of veterinary drug residue towards high automation, high sensitivity, and high throughput in the future. This review provides new ideas and strategies for the rapid development of animal husbandry industry and protecting consumers’ physical health and food safety.

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A sensitive and specific enzyme-linked immunosorbent assay for the detection of pymetrozine in vegetable, cereal, and meat

Cited by 21 publications

References 11 publications

A Sensitive and Specific Monoclonal Antibody Based Enzyme-Linked Immunosorbent Assay for the Rapid Detection of Pretilachlor in Grains and the Environment

A Sensitive and Specific Monoclonal Antibody Based Enzyme-Linked Immunosorbent Assay for the Rapid Detection of Pretilachlor in Grains and the Environment

Rapid Detection of Prometryn Residues in Agricultural Samples: Method Comparison of the ic-ELISA and Colloidal Gold Immunochromatographic Test Strips

A Review of Veterinary Drug Residue Detection: Recent Advancements, Challenges, and Future Directions

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