A sensitive and specific assay for superoxide anion released by neutrophils or macrophages based on bioluminescence of polynoidin

Colepicolo, Pio; Camarero, V C P C; Nicolas, Marie‐Thérèse; Bassot, Jean‐Marie; Karnovsky, Manfred L.; Hastings, J. Woodland

doi:10.1016/0003-2697(90)90695-6

Cited by 17 publications

(6 citation statements)

References 42 publications

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“…Other important sources or reactive oxygen species are the reaction catalysed by xantine oxidase in the conversion of hypoxanthine to xanthine and uric acid, as well as the activation of NADPH oxidase of polymorphonuclear cells during superoxide production and the process of phagocytosis (Colepicolo et al, 1990;Uyama et al, 1990). Singlet oxygen appears to be involved in the non-enzymatic destruction of catecholamines (Kruck et al, 1989) as well as in other oxidative processes (Patterson et al, 1990).…”

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confidence: 96%

“…dipyridamole; diethyldithiocarbamate; catechin; vitamin E I NTRO D UCTlO N Superoxide, hydroxyl radicals and alkoxy radicals can be considered to be the most important reactive oxygen species produced in biological systems by diverse metabolic pathways including microsomes, mitochondria1 electron transport, and lipooxygenases and cycloxygenases in eicosanoid metabolism. Other important sources or reactive oxygen species are the reaction catalysed by xantine oxidase in the conversion of hypoxanthine to xanthine and uric acid, as well as the activation of NADPH oxidase of polymorphonuclear cells during superoxide production and the process of phagocytosis (Colepicolo et al, 1990;Uyama et al, 1990). Singlet oxygen appears to be involved in the non-enzymatic destruction of catecholamines (Kruck et al, 1989) as well as in other oxidative processes (Patterson et al, 1990).…”

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confidence: 99%

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Effect of antioxidants on chemiluminescence produced by reactive oxygen species

Pascual¹,

Romay²

1992

J. Biolumin. Chemilumin.

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Luminol chemiluminescence was used to evaluate the scavenging of superoxide, hydroxyl and alkoxy radicals by four antioxidants: dipyridamole, diethyldithiocarbamic acid, (+)catechin, and ascorbic acid. Different concentrations of these compounds were compared with well-known oxygen radical scavengers in their capacity to inhibit the chemiluminescence produced in the reaction between luminol and specific oxygen radicals. Hydroxyl radicals were generated using the Fenton reaction and these produced chemiluminescence which was inhibited by diethyldithiocarbamate. Alkoxy radicals were generated using the reaction of tert-butyl hydroperoxide and ferrous ion and produced chemiluminescence which was inhibited equally by all of the compounds tested. For the determination of superoxide scavengers we describe a new, simple, economic, and rapid chemiluminescence method consisting of the reaction between luminol and horseradish peroxidase (HRP). With this method it was found that 40 nmol/l dipyridamole, 0.18 mumol/l ascorbic acid, 0.23 mumol/l (+)catechin, and 3 mumol/l diethyldithiocarbamic acid are equivalent to 3.9 ng/ml superoxide dismutase (specific scavenger of superoxide) in causing the same degree of chemiluminescence inhibition. These results not only indicated that the antioxidative properties of these compounds showed different degrees of effectiveness against a particular radical but also that they may exert their action against more than one radical.

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confidence: 96%

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confidence: 99%

Effect of antioxidants on chemiluminescence produced by reactive oxygen species

Pascual¹,

Romay²

1992

J. Biolumin. Chemilumin.

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“…A sensitive and specific bioluminescence of protein polynoidin for O 2 •has also been developed during the past decades and utilized limitedly in cell biology and medicine. 24 a conversion of superoxide radical to the hydroxyl radical by superoxide-driven Fenton reactions in 60 CO γ-radiation as measured by the formation of fluorescent hydroxylated derivatives from benzoate. However, this method has only focused on the conversion effect of superoxide radical by ferric ethylenediamineacetate (Fe 3+ -EDTA) as the catalyst, rather than the absolute concentration of superoxide radical.…”

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confidence: 99%

“…Among enzymatic methods, the most commonly used involves the inhibition of lipid peroxidation by antioxidants, which can be measured by the end products, adducts, or other indicators of oxidative reaction. ,− However, enzymatic methods are disadvantageous as the enzymes are unstable and expensive. A sensitive and specific bioluminescence of protein polynoidin for O 2 •- has also been developed during the past decades and utilized limitedly in cell biology and medicine . Baker and Gebicki 25 characterized a conversion of superoxide radical to the hydroxyl radical by superoxide-driven Fenton reactions in 60 CO γ-radiation as measured by the formation of fluorescent hydroxylated derivatives from benzoate.…”

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A Kinetic Method for HO₂^•/O₂^•^- Determination in Advanced Oxidation Processes

Kwon

Lee

2004

Anal. Chem.

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A new kinetic method is developed for the determination of hydroperoxyl radical (HO(2)(*))/superoxide radical (O(2)(*)(-)) in aqueous solution, and the calibration using a kinetic half-life technique is also established for determining the concentration of HO(2)(*)/O(2)(*)(-) as produced in the UV/H(2)O(2) process. This new method is based on the reduction of Fe(3+)-EDTA into Fe(2+)-EDTA by HO(2)(*)/O(2)(*)(-) and the well-known Fenton-like reaction of H(2)O(2) and Fe(2+)-EDTA to yield the hydroxyl radicals (OH(*)). Benzoic acid scavenges the OH radicals to produce hydroxybenzoic acids, which are analyzed by fluorescence detection (lambda(ex) = 320 nm; lambda(em) = 400 nm). The limit of detection for the new method depends on the pH values, and it is determined as 3.22 x 10(-)(11) M with signal-to-noise ratio of 2 at pH 5. In addition, the present technique has the advantage of using inexpensive and easily available nonenzymatic reagents that do not require the specific instrument and chemicals and of being insensitive to the moderate concentration of possible interferences often found in aqueous phase.

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“…Rejection response involves infiltration of inflammatory cells, i.e., lymphocytes, monocytes, and polymorphonuclear neutrophils (PMNs), at the graft site. There is much evidence that activatedmonocytes and PMNs can generate the oxygen free radical [10,18,26], which is toxic to biomolecules of the grafted organ. In addition, impairment of coronary flow and myocytic necrosis developed during acute rejection in non-immunosuppressed animals [6,7,27], although the coronary flow was not observed to decrease in the presence of mild or moderate rejection, which had occurred after a 4-daycessation ofimmunosuppression [5].…”

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confidence: 99%

Rescue therapy for acute rejection using 15-deoxyspergualin (DSG) in combination with superoxide dismutase (SOD) on cardiac allografts in rats

Suzuki¹,

Kanashiro

Hayashi

et al. 1992

Transplant International

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This study was performed to investigate the effect of combination therapy using 15-deoxyspergualin (DSG) plus recombinant human superoxide dismutase (h-SOD) on acute graft rejection in heterotopic rat heart transplantation. DSG was intraperitoneally injected for 10 days at a dose of 5 mg/kg per day, and h-SOD (15,000 or 30,000 flmlkg per day) was continuously administered via external iliac vein for approximately 8 days with a miniosmotic pump (Alzet model2001). Administration of the drugs was started on the 4th day after grafting. The grafts treated with h-SOD alone survived slightly longer than the control allografts. The graft survival time was significantly prolonged in the groups treated with DSG alone or DSG plush-SOD. A higher percentage of induction of immunological unresponsiveness was achieved in the group treated with DSG plus h-SOD at 30,000 f.!m/kg per day. The ratios of inorganic phosphate (Pi)/phosphocreatine (PCr) and PCr/ATP on the 31 P nuclear magnetic resonance spectrogram are useful parameters for assessing the graft injury associated with acute rejection. The ratio of Pi!PCr and that of PCr/ ATP were found to increase and decrease, respectively, in proportion to the progress in rejection. In the animals treated with DSG alone, the Pi/PCr ratio was significantly increased from the 4th day, and PCr/ATP ratio decreased from lOth day after grafting. These parameters were not improved during the observation period. However, these parameters were significantly recovered in the animals treated with DSG and h-SOD in combination. Improvement of the parameters seemed to be related to SOD dosage.These results clearly demonstrated that the oxygen free radical plays a toxic role in cardiac allografts with ongoing rejection and, therefore, the administration of h-SOD in combination with DSG can minimize the graft injury.

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A sensitive and specific assay for superoxide anion released by neutrophils or macrophages based on bioluminescence of polynoidin

Cited by 17 publications

References 42 publications

Effect of antioxidants on chemiluminescence produced by reactive oxygen species

Effect of antioxidants on chemiluminescence produced by reactive oxygen species

A Kinetic Method for HO₂^•/O₂^•^- Determination in Advanced Oxidation Processes

Rescue therapy for acute rejection using 15-deoxyspergualin (DSG) in combination with superoxide dismutase (SOD) on cardiac allografts in rats

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A sensitive and specific assay for superoxide anion released by neutrophils or macrophages based on bioluminescence of polynoidin

Cited by 17 publications

References 42 publications

Effect of antioxidants on chemiluminescence produced by reactive oxygen species

Effect of antioxidants on chemiluminescence produced by reactive oxygen species

A Kinetic Method for HO2•/O2•- Determination in Advanced Oxidation Processes

Rescue therapy for acute rejection using 15-deoxyspergualin (DSG) in combination with superoxide dismutase (SOD) on cardiac allografts in rats

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A Kinetic Method for HO₂^•/O₂^•^- Determination in Advanced Oxidation Processes