A sensitive and simplified HPLC analysis for the determination of fluconazol in human plasma

Kim, Eun J.; Lee, Hye S.; Zee, Ok Pyo; Lee, Seung Tae

doi:10.1007/bf02861318

Cited by 9 publications

(3 citation statements)

References 1 publication

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“…HPLC proved to be particularly suitable for monitoring of fl uconazole in biological fl uids [10,12] . Bioassays are a time-consuming method and not very reliable, because in vitro medium conditions infl uence the antifungal activity of fl uconazole [10] .…”

Section: Discussionmentioning

confidence: 99%

“…Liquid-and gas-chromatographic methods for fl uconazole determination in human plasma or serum have been reported to monitor the drug concentrations in biological materials [10,12] . Microbiological determination of chemotherapeutics is not appropriate for pharmacokinetic studies, because it is not selective since the metabolites and endogenous compounds interfere with assay techniques [10,13] .…”

Section: Introductionmentioning

confidence: 99%

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Pharmacokinetics of Fluconazole in the Cerebrospinal Fluid of Children with Hydrocephalus

Bafeltowska¹,

Buszman²

2005

Chemotherapy

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Background: The aim of this study was to examine the pharmacokinetics of fluconazole in the CSF of children with hydrocephalus during CNS infection treatment after intravenous and/or intraventricular drug administration. Direct fluconazole administration into the ventricular CSF of patients to treat serious CNS infections is an aggressive therapy, and data on the pharmacokinetics of fluconazole in CSF are limited. Methods: A method of fluconazole quantification in CSF by solid-phase extraction (SPE)-high-performance liquid chromatography (HPLC) was developed to conduct pharmacokinetic studies. The population of patients included 2 children with hydrocephalus. Fluconazole was administered intravenously at average multiple doses of 12.5 mg/kg/24 h and intraventricularly at doses of 4, 5 and 7.5 mg/24 h, and 7.5 and 10 mg/12 h. The CSF samples were taken 2–24 h after administration of fluconazole. The concentrations of fluconazole in CSF specimens were assessed, and after pharmacokinetic studies the fluconazole dosage was modified. Results: The method of fluconazole determination in CSF using the SPE-HPLC method is specific, precise and accurate. After intravenous fluconazole administration, the concentration of this antifungal drug was not detected in the ventricular CSF. The pharmacokinetic parameters determined after intraventricular fluconazole administration were: steady-state peak CSF fluconazole concentration (19.54 ± 5.63 mg/l); trough CSF fluconazole concentration (0.0–0.3 mg/l); elimination rate constant (0.4654 ± 0.2097 h^–1), and half-life (1.84 ± 0.93 h). Conclusions: The authors developed a method to determine fluconazole in CSF by SPE-HPLC. After intravenous fluconazole administration, the drug was not detected in the examined CSF samples. The intraventricular multidose pharmacokinetic data suggest the necessity of fluconazole monitoring in children with hydrocephalus during the treatment of shunt infection.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Pharmacokinetics of Fluconazole in the Cerebrospinal Fluid of Children with Hydrocephalus

Bafeltowska¹,

Buszman²

2005

Chemotherapy

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“…Thus, vali-dation is used to demonstrate that the analytical technique provides reliable results through a systematic evaluation of the method [25]. Several methods are found in the literature for the quantification of CUR [28][29][30] and FL [31][32][33][34] by HPLC. However, to date, there is no simple, fast and sensitive analytical method for the simultaneous quantification of FL and CUR in the literature, neither in solution nor in nanostructures.…”

Section: Introductionmentioning

confidence: 99%

Validation of an innovative analytical method for simultaneous quantification of curcumin and fluconazole using high‐performance liquid chromatography from nanostructured lipid carriers

Araújo

Fernandes

Reis

et al. 2021

J of Separation Science

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Vulvovaginal candidiasis is a public health problem with a high incidence among female patients. Currently, there is an increase in the identification of Candida spp. resistant to current therapy, making it necessary to search for new therapeutic alternatives. The synergistic potential of curcumin with fluconazole is described in the literature. However, due to its high lipophilicity, it is necessary to use drug-delivery systems to adequately explore its potential, among which is the nanostructured lipid carrier. However, to date, there is no validated method of high-performance liquid chromatography for simultaneous determination of fluconazole and curcumin in the literature. Thus, the present work developed a high-performance liquid chromatography method for simultaneous determination of fluconazole and curcumin co-encapsulated in nanostructured lipid carrier which was validated according to the International Conference on Harmonization (Technical Requirements for Registration of Pharmaceuticals for Human Use) -Q2 (R1) and the Food and Drug Administration -Guidance for Bioanalytical Method. The method was applied to determine the encapsulation efficiency and drug-loading of curcumin and fluconazole in nanostructured lipid carriers.The developed method proved to be selective, precise, accurate, and robust for the simultaneous determination of both drugs, enabling the quantification of encapsulation efficiency and drug-loading of curcumin and fluconazole in nanostructured lipid carriers.

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Particle beam-mass spectrometric analysis of difluorophenyl triazole compounds using normal phase-HPLC

Creaser

Stygall

Bowen

et al. 1997

Talanta

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A sensitive and simplified HPLC analysis for the determination of fluconazol in human plasma

Cited by 9 publications

References 1 publication

Pharmacokinetics of Fluconazole in the Cerebrospinal Fluid of Children with Hydrocephalus

Pharmacokinetics of Fluconazole in the Cerebrospinal Fluid of Children with Hydrocephalus

Validation of an innovative analytical method for simultaneous quantification of curcumin and fluconazole using high‐performance liquid chromatography from nanostructured lipid carriers

Particle beam-mass spectrometric analysis of difluorophenyl triazole compounds using normal phase-HPLC

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