A sensitive and selective HPLC-FLD method with fluorescent labeling for simultaneous detection of bile acid and free fatty acid in human serum

Li, Guoliang; Chen, Guang; Liu, Yueqiu; Jing, Nianhua; You, Jing

doi:10.1016/j.jchromb.2012.03.029

Cited by 12 publications

(6 citation statements)

References 32 publications

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“…In comparison with the MS system, the mobile phase can contain nonvolatile components, expenditures for the UV‐VIS detector are lower and maintenance is simpler, but the sensitivity is worse and, thus, derivatization of BAs with chromophore has to be applied [45,60]. On the other hand, FLD displays sensitivity comparable to HPLC‐MS, but sample preparation is more time consuming as BA derivatization is necessary [63,64].…”

Section: Bile Acid Analysismentioning

confidence: 99%

Analysis of bile acids in human biological samples by microcolumn separation techniques: A review

2020

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Bile acids are a group of compounds essential for lipid digestion and absorption with a steroid skeleton and a carboxylate side chain usually conjugated to glycine or taurine. Bile acids are regulatory molecules for a number of metabolic processes and can be used as biomarkers of various disorders. Since the middle of the twentieth century, the detection of bile acids has evolved from simple qualitative analysis to accurate quantification in complicated mixtures. Advanced methods are required to characterize and quantify individual bile acids in these mixtures. This article overviews the literature from the last two decades (2000-2020) and focuses on bile acid analysis in various human biological samples. The methods for sample preparation, including the sample treatment of conventional (blood plasma, blood serum, and urine) and unconventional samples (bile, saliva, duodenal/gastric juice, feces, etc.) are shortly discussed. Eventually, the focus is on novel analytical approaches and methods for each particular biological sample, providing an overview of the microcolumn separation techniques, such as high-performance liquid chromatography, gas chromatography, and capillary electrophoresis, used in their analysis. This is followed by a discussion on selected clinical applications.

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Section: Bile Acid Analysismentioning

confidence: 99%

Analysis of bile acids in human biological samples by microcolumn separation techniques: A review

2020

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“…To monitor the FAs, many approaches have been employed using various analytical techniques including thin layer chromatography, GC and/or GC‐MS, liquid chromatography (LC) and/or LC coupled with MS, and so on. However, due to the feature of the structure, FAs are difficult to be directly separated and determined by GC.…”

Section: Introductionmentioning

confidence: 99%

“…[2][3][4][5] In addition to providing energy, long chain FA can affect numerous cellular signaling and metabolic pathways such as structural components of biological membranes, mediators of signal transduction and transcription, and physiological regulators. [6] To monitor the FAs, many approaches have been employed using various analytical techniques including thin layer chromatography, [7,8] GC and/or GC-MS, [9][10][11][12] liquid chromatography (LC) and/or LC coupled with MS, [13][14][15][16][17][18] and so on. However, due to the feature of the structure, FAs are difficult to be directly separated and determined by GC.…”

Section: Introductionmentioning

confidence: 99%

Characterizing ion mobility and collision cross section of fatty acids using electrospray ion mobility mass spectrometry

Zhang

Guo

Zhang³

et al. 2015

J. Mass Spectrom.

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This study investigated the ion mobility (IM) and the collision cross section (CCS) of fatty acids (FAs) using electrospray IM MS. The IM analysis of 18 FA ions showed intriguing differences among the saturated FAs, monounsaturated FAs, multi-unsaturated FAs, and cis-isomer/trans-isomer with respect to the aliphatic tail chains. The length of aliphatic tail chain present in the ion structures had a strong influence on the differentiation of drift, while the number of double bond showed a weaker influence. The tiny drift differences between cis-isomer and trans-isomer were also observed. In the CCS measurements, two internal standards were involved in the mobility calibration and accuracy estimation. It insured our empirical CCS values were of high experimental precision (±0.35% or better) and accuracy (±0.25% or better). Moreover, the mass-to-charge ratio (m/z) - mobility plots obtained by ion mobility spectrometry with mass spectrometry analysis of FAs - was used to investigate the structural relationship between the molecules. Each series of FAs sharing a similar structure was aligned in the linear plot. Finally, the developed procedure was applied to the determination of FAs in rat adipose tissues, and it allowed the presence of 13 FAs to be confirmed with their exact masses and CCS values. These studies reveal the direct relationship between the behaviors in IM and the molecular structures and thus may provide further validations to the FA identification process.

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“…One of the strategies for separating enantiomeric isomers is the chiral derivatization method. (+)-O,O'-Diacetyl-L-tartaric anhydride (DATAN) [77] and N-(ptoluenesulfonyl)-L-phenylalanyl chloride (TSPC) [78] are two reagents developed for this purpose. Both reagents have been used for RPLC-MS/MS analysis.…”

Section: α-Keto Acids and Their Metabolitesmentioning

confidence: 99%

“…Concentrations of deoxycholic acid and chenodeoxycholic acid ( Figure 15) in human plasma were 91 and 445 nM, respectively. 2-(7H-Dibenzo[a,g]carbazol-7-yl)ethyl 4-methylbenzenesulfonate (DBCETS) derivatization was also performed for fluorescence detection to determine bile acid concentrations in human serum [77]. Derivatization with 2-bromo-4 -nitroacetophenone and phenacyl bromide was also used for UV detection [78,79].…”

Section: Miscellaneousmentioning

confidence: 99%

Liquid-Chromatographic Methods for Carboxylic Acids in Biological Samples

et al. 2020

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Carboxyl-bearing low-molecular-weight compounds such as keto acids, fatty acids, and other organic acids are involved in a myriad of metabolic pathways owing to their high polarity and solubility in biological fluids. Various disease areas such as cancer, myeloid leukemia, heart disease, liver disease, and lifestyle diseases (obesity and diabetes) were found to be related to certain metabolic pathways and changes in the concentrations of the compounds involved in those pathways. Therefore, the quantification of such compounds provides useful information pertaining to diagnosis, pathological conditions, and disease mechanisms, spurring the development of numerous analytical methods for this purpose. This review article addresses analytical methods for the quantification of carboxylic acids, which were classified into fatty acids, tricarboxylic acid cycle and glycolysis-related compounds, amino acid metabolites, perfluorinated carboxylic acids, α-keto acids and their metabolites, thiazole-containing carboxylic acids, and miscellaneous, in biological samples from 2000 to date. Methods involving liquid chromatography coupled with ultraviolet, fluorescence, mass spectrometry, and electrochemical detection were summarized.

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A sensitive and selective HPLC-FLD method with fluorescent labeling for simultaneous detection of bile acid and free fatty acid in human serum

Cited by 12 publications

References 32 publications

Analysis of bile acids in human biological samples by microcolumn separation techniques: A review

Analysis of bile acids in human biological samples by microcolumn separation techniques: A review

Characterizing ion mobility and collision cross section of fatty acids using electrospray ion mobility mass spectrometry

Liquid-Chromatographic Methods for Carboxylic Acids in Biological Samples

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