A Sensitive and Selective Chromogenic Organic Reagent 4-hydroxy-3,5-dimethoxy benzaldehyde-4-hydroxy benzoyl hydrazone (HDMBHBH) for the Direct and Derivative Spectrophotometric Determination of Lead (II)

Abstract

4-hydroxy-3,5-dimethoxy benzaldehyde-4-hydroxy benzoyl hydrazone (HDMBHBH) is used as a novel chromogenic organic reagent for the determination of Lead (II) using spectrophotometry. The novel chromogenic organic reagent 4-hydroxy-3,5-dimethoxy benzaldehyde-4-hydroxy benzoyl hydrazone (HDMBHBH) gave yellow coloured water soluble complex with Pb (II) in basic buffer (pH = 10.0) medium. The colour complex shows maximum absorbance at 386 nm. The system obeyed beer's law in the concentration range of 0.518-5.18g/ml. The optimum Lead (II) concentration range for accurate determination as evaluated from Ringbom plot was 1.036-4.662 g/ml. The molar absorptivity and Sandell's sensitivity were 2.6610 4 L . mol -1. cm -1 and 0.0077 g/cm 2 respectively. The Lead (II) forms I:I colour complex with HDMBHBH and stability constant of the complex was found to be 3.4210 6 . The present developed method was successfully applied for the determination of Lead (II) in biological samples.