A sensitive and rapid detection of glutathione based on a fluorescence-enhanced “turn-on” strategy

Ma, Qianru; Wang, Minning; Cai, Huahuan; Li, Fulai; Fu, Songsen; Liu, Yan; Zhao, Yufen

doi:10.1039/d1tb00232e

Cited by 15 publications

(4 citation statements)

References 45 publications

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“…Glutathione (GSH) is an important biomolecule and plays a vital role in mammalian and eukaryotic cells by protecting cells from reactive oxygen species (ROS) and toxins. The imbalance between GSH to GSSH (oxidized glutathione) suggests that the cells suffer from oxidative stress, and deviation of the ratio of GSH to GSSG from its standard range is associated with various clinical diseases. ,− Therefore, GSH can be used as clinical biomarkers and sensitive detection of GSH would provide us with point-of-care (PoC) diagnostics for numerous diseases. Because of the presence of free thiol groups in reduced glutathione, it exhibits important reducing properties.…”

Section: Resultsmentioning

confidence: 99%

Two-Dimensional Ultrathin CeVO₄ Nanozyme: Fabricated through Non-Oxidic Material

et al. 2023

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In recent years, the synthesis of materials in lower dimensions, like two-dimensional (2D) or ultrathin crystals, with distinctive characteristics has attracted substantial scientific attention. The mixed transition metal oxides (MTMOs) nanomaterials are the promising group of materials, which have been extensively utilized for various potential applications. Most of the MTMOs were explored as three-dimensional (3D) nanospheres, nanoparticles, one-dimensional (1D) nanorods, and nanotubes. However, these materials are not well explored in 2D morphology because of the difficulties in removing tightly woven thin oxide layers or exfoliations of 2D oxide layers, which hinder the exfoliation of beneficial features of MTMO. Here, through the exfoliation via Li + ion intercalation and subsequent oxidation of CeVS 3 under hydrothermal condition, we have demonstrated a novel synthetic route for the fabrication of 2D ultrathin CeVO 4 NS. The as-synthesized CeVO 4 NS exhibit adequate stability and activity in a harsh reaction environment, which gives excellent peroxidase-mimicking activity with a K M value of 0.04 mM, noticeably better than natural peroxidase and previously reported CeVO 4 nanoparticles. We have also used this enzyme mimic activity for the efficient detection of biomolecules like glutathione with a LOD of 53 nM.

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Section: Resultsmentioning

confidence: 99%

Two-Dimensional Ultrathin CeVO₄ Nanozyme: Fabricated through Non-Oxidic Material

et al. 2023

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“…The LOD and LOQ were found to be 15 pM and 46 pM, respectively. The detection limits and linear range of detection of GSH using other materials are summarized in Table S6. − …”

Section: Resultsmentioning

confidence: 99%

Biogenic Carbon Dot-Embedded Chitosan Hydrogels as a Two-Stage Fluorescence OFF–ON Probe for Sequential and Ratiometric Detection of Fe(III) and Glutathione

Thakkar,

Bhandary,

Thakore

2023

ACS Appl. Nano Mater.

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Biogenic carbon dots (BCDs) are useful fluorescence sensors for selective detection of toxic metals. In the present work, we propose to synthesize a hybrid fluorescent chitosan gel using BCDs for detection and enrichment of Fe(III) ions and subsequent ratiometric detection of glutathione (GSH). Carbon dots were synthesized using waste lemon peels (LCDs) as the precursor. The sensing studies showed the selectivity and ultrasensitivity of the LCD sensor toward Fe(III) in the presence of other metal ions with a limit of detection as low as 17 pM. The visual as well as PL studies demonstrated that the LCD sensing probe is free from any significant interference. The LCDs were successfully fabricated to develop “in-house” paper-based kits for in situ and on-site detection of Fe(III). Further, a fluorescent chitosan-based hydrogel (CDG) was synthesized by conjugation of LCDs for the selective uptake and enrichment of Fe(III), which led to quenching of fluorescence with >90% encapsulation. Post enrichment, the Fe(III) enriched LCD hybrid hydrogel (Fe@CDG) was further applied for the selective and ultrasensitive detection of GSH with the limit of detection of 15.22 pM. The sensing studies showed that the detection of GSH is free from any interference. Thus, the fluorescent hydrogel exhibited quenching on entrapment of Fe(III) (Turn OFF) and recurrence of fluorescence on the release of entrapped Fe(III) in the presence of GSH (Turn ON). Additionally, LCDs and the CDG demonstrated potential antibacterial activity, annihilating bacteria in water samples in less than 10 min of photoexcitation, due to the potential ROS generation ability of the LCDs.

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“…The capacity of existing analytical methods to provide quick, consistent quantitative measurements of thiol biomarkers is essential for such a glutathione monitoring system. Number of analytical methods including high performance liquid chromatography [41][42][43][44], enzymatic method [45], electrochemical methods [46][47][48], capillary electrophoresis [44], spectrofluorimetry [49,50], proton nuclear magnetic resonance [51], surface-enhanced Raman scattering [52], flow injection analysis [53,54] are available for the detection of glutathione. Electrochemical assays have low stability and are difficult to operate.…”

Section: Introductionmentioning

confidence: 99%

Probing nanometal surface energy transfer between nanocopper and MoS₂ for glutathione sensing

Archana¹,

Karthikeyan²

2022

J. Phys. D: Appl. Phys.

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Cu nanoparticles and 2D-MoS2 nanosheets have been synthesised and the morphology of the prepared samples has been characterised using High-Resolution Transmission Electronf Microscopy and Field Emission Scanning Electron Microscopy. Steady-state fluorescence studies exhibit quenching of fluorescence intensity upon the addition of quencher in varying amounts. Time-resolved fluorescence studies show a reduction of fluorescence lifetime in the presence of quencher. A thorough investigation of energy transfer between MoS2 and copper nanoparticles has been accomplished using steady-state and time-resolved fluorescence studies. The results are more consistent with the Nanometal surface energy transfer theory, which follows a 1/d4 distance dependence than the Foster Resonance energy transfer theory exhibiting 1/d6 distance dependence. For the MoS2-Cu pair, the Nanometal surface energy transfer efficiency, the proximal distance between donor-acceptor pairs and the rate of energy transfer has been explored. Energy transfer-based fluorescent biosensors have been regarded as potential candidates in biosensing applications. Glutathione, an endogenous tripeptide compound, plays a vital role as an antioxidant in the human body. Irregular glutathione levels have been linked to several adverse illnesses. Henceforth, detection of glutathione levels is indispensable. The Cu-MoS2 pair was used for glutathione sensing in nanomolar concentration (nM), which demonstrated a turn-off sensing behaviour.

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A sensitive and rapid detection of glutathione based on a fluorescence-enhanced “turn-on” strategy

Abstract: Glutathione (GSH) plays important roles in human body including protecting cells from oxidative damages and maintaining cellular redox homeostasis. Thus, developing a fast and sensitive method to detect GSH level...

Cited by 15 publications

References 45 publications

Two-Dimensional Ultrathin CeVO₄ Nanozyme: Fabricated through Non-Oxidic Material

Two-Dimensional Ultrathin CeVO₄ Nanozyme: Fabricated through Non-Oxidic Material

Biogenic Carbon Dot-Embedded Chitosan Hydrogels as a Two-Stage Fluorescence OFF–ON Probe for Sequential and Ratiometric Detection of Fe(III) and Glutathione

Probing nanometal surface energy transfer between nanocopper and MoS₂ for glutathione sensing

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A sensitive and rapid detection of glutathione based on a fluorescence-enhanced “turn-on” strategy

Abstract: Glutathione (GSH) plays important roles in human body including protecting cells from oxidative damages and maintaining cellular redox homeostasis. Thus, developing a fast and sensitive method to detect GSH level...

Cited by 15 publications

References 45 publications

Two-Dimensional Ultrathin CeVO4 Nanozyme: Fabricated through Non-Oxidic Material

Two-Dimensional Ultrathin CeVO4 Nanozyme: Fabricated through Non-Oxidic Material

Biogenic Carbon Dot-Embedded Chitosan Hydrogels as a Two-Stage Fluorescence OFF–ON Probe for Sequential and Ratiometric Detection of Fe(III) and Glutathione

Probing nanometal surface energy transfer between nanocopper and MoS2 for glutathione sensing

Contact Info

Product

Resources

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Two-Dimensional Ultrathin CeVO₄ Nanozyme: Fabricated through Non-Oxidic Material

Two-Dimensional Ultrathin CeVO₄ Nanozyme: Fabricated through Non-Oxidic Material

Probing nanometal surface energy transfer between nanocopper and MoS₂ for glutathione sensing