A Sensitive and Effective Proteomic Approach to Identify She-Donkey’s and Goat’s Milk Adulterations by MALDI-TOF MS Fingerprinting

Girolamo, Francesco; Masotti, Andrea; Salvatori, Guglielmo; Scapaticci, Margherita; Muraca, Maurizio; Putignani, Lorenza

doi:10.3390/ijms150813697

Cited by 35 publications

(20 citation statements)

References 23 publications

(28 reference statements)

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“…The proteomics method as an emerging method with high sensitivity and selectivity has also been applied in the determination of milk adulteration. This method focuses on qualitative (Di Girolamo et al, 2014;Bernardi et al, 2015) or semiquantitative (Camerini et al, 2016) analysis. Milk adulteration driven by economic reasons affects the percentage of milk in dairy products.…”

Section: Introductionmentioning

confidence: 99%

Proteomics method to quantify the percentage of cow, goat, and sheep milks in raw materials for dairy products

Chen

Xing

Zhang

et al. 2016

Journal of Dairy Science

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Fraud in milk and dairy products occurs when cow milk is added to sheep and goat milk for economic reasons. No reliable, selective, and sensitive method exists for quantifying the milk percentage of different species. This work reports the development and validation of a proteomics-based method for the qualitative detection and quantitative determination of cow, sheep, and goat milks in the raw materials used for dairy products. β-Lactoglobulin was selected as the protein marker because it is a major protein in milk and whey powder. The tryptic peptides LSFNPTQLEEQCHI and LAFNPTQLEGQCHV were used as signature peptides for cow milk and for sheep and goat milks, respectively. The winged peptides LKALPMHIRLSFNPTQL*EEQCHI* and LKALPMHIRLAFNPTQL*EGQCHV* were designed and synthesized as internal standards. Validation of the method showed that it has good sensitivity, specificity, reproducibility, precision, and accuracy. This method is easily applicable in routine laboratory analysis without intensive proteomics background.

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Section: Introductionmentioning

confidence: 99%

Proteomics method to quantify the percentage of cow, goat, and sheep milks in raw materials for dairy products

Chen

Xing

Zhang

et al. 2016

Journal of Dairy Science

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“…Among other applications, it has been used to determine the origin of food [69][70][71] and food adulterations [72][73][74][75][76][77]. The discrimination of samples on the basis of their protein fingerprints using MALDI-MS is also an intense area of research [78][79][80].…”

Section: Direct Apci-hrms and Ptr-hrmsmentioning

confidence: 99%

“…Similar limits of adulteration (ca. 0.5 %) were also obtained through MALDI-TOF MS making it possible to detect adulteration of both donkey and goat milks with other species' milks (cow, ewe, and buffalo) on the basis of their different proteomic profiles [77].…”

Section: Direct Apci-hrms and Ptr-hrmsmentioning

confidence: 99%

The role of direct high-resolution mass spectrometry in foodomics

et al. 2015

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Foodomics has been defined as a global discipline in which advanced analytical techniques and bioinformatics are combined to address different questions in food science and nutrition. There is a growing number of works on the development and application of non-targeted omics methods in foodomics, which reflects that this emerging discipline is already considered by the scientific community to be a valuable approach to assess food safety, quality, and traceability as well as for the study of the links between food and health. As a result, there is a clear need for more rapid, high-throughput MS approaches for developing and applying non-targeted studies. Nowadays, direct MS analysis is one of the main choices to achieve high throughput, generating a set of information from the largest possible number of samples in a fast and straightforward way. The use of high- and ultrahigh-resolution MS greatly improves the analytical performance and offers a good combination of selectivity and sensitivity. By using a range of methods for direct sample introduction/desorption/ionization, high-throughput and non-target analysis of a variety of samples can be obtained in a few seconds by HRMS analysis. In this review, a general overview is presented of the main characteristics of direct HRMS-based approaches and their principal applications in foodomics.

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“…To detect adulteration of goat's milk with cow's milk the reference method is used, which is based on the differentiation of isoelectric points of cow and goat γ 2 -and γ 3 -caseins originating from the hydrolysis of the belonging β-caseins (Commision Regulation 2008; EC 273/08). But there are many studies, where mass spectrometry (MS) (C hen et al, 2004; C alvano et al, 2012; Girolamo et al, 2014) or gel based approaches (Hinz et al, 2012;Špoljarić et al, 2013;Yang et al, 2014) have been used to detect adulteration of goat's milk with cow's milk or just for comparing protein profiles of different animal species milks. Although the general tendency nowadays is to move to gel-free workflows, protein electrophoresis has been used extensively (Ortea et al, 2012) and continues to be used (mainly 2-DE) in food authentication studies (Ortea et al, 2016).…”

Section: Introductionmentioning

confidence: 99%

Potential of bovine kappa - casein as biomarker for detection of adulteration of goat’s milk with cow’s milk

et al. 2019

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Using two-dimensional (2-D) electrophoresis it was shown that bovine kappa-casein could be an appropriate biomarker of adulteration of goat’s milk with cow’s milk not only in raw milk, but also for milk thermally processed by pasteurization or treated with ultra-high-temperature. The presence of cow’s milk in goat’s milk was detected at level of 2 %. Furthermore, position of bovine kappa-casein spots on 2-D gels remained unchanged even with samples from two different geographical origins, Belgium and Slovenia. These results show that neither thermal processing nor different geographical area seem to affect the position of bovine kappa-casein spots on 2-D gels.

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A Sensitive and Effective Proteomic Approach to Identify She-Donkey’s and Goat’s Milk Adulterations by MALDI-TOF MS Fingerprinting

Cited by 35 publications

References 23 publications

Proteomics method to quantify the percentage of cow, goat, and sheep milks in raw materials for dairy products

Proteomics method to quantify the percentage of cow, goat, and sheep milks in raw materials for dairy products

The role of direct high-resolution mass spectrometry in foodomics

Potential of bovine kappa - casein as biomarker for detection of adulteration of goat’s milk with cow’s milk

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