A semi-structured model for the growth and β-Galactosidase production by fed-batch fermentation of Kluyveromyces marxianus

Abstract

A model is proposed to describe the growth and b-Galactosidase production by Kluyveromyces marxianus. It shows good agreement with the experimental data over a range of conditions. It also reliably predicts the effect of growth rate on the productivity of the protein. The simplicity and accuracy of the model make it suitable for designing and implementing control and optimization strategies for the production of protein at large scale.

Enzyme Production

“…Fed-batch fermentation is a variant of the former in which, after certain time of batch cultivation, the bioreactor is fed with nutrients according to a controlled rate profile and up to a final volume and the product is then recovered as above. This mode of cultivation is particularly appealing for the production of enzymes because it allows the control of the metabolic responses of the producing cells and operation is rather simple (Moon and Parulekar 1991;Acevedo and Gentina 1996;Bojorge et al 1999;Cereghino et al 2002). The third mode is continuous culture, in which the medium is fed continuously to the bioreactor and the fermented broth continuously removed at the same rate.…”

Comparison of the Gene Expression Patterns of Alcohol Dehydrogenase Isozymes in the Thermotolerant YeastKluyveromyces marxianusand Their Physiological Functions

“…18,19) This yeast is also capable of growing on lactose as sole carbon source, which provides the possibility of utilizing cheese whey as a cheap source of nutrients for its cultivation. 20) K. marxianus has been investigated for the production of bioingredients, 18) cell proteins, 21,22) inulinase, 16,[23][24][25] and -galactosidase, [26][27][28] and even for use as a baker's yeast. 29) In spite of these potential applications of K. marxianus, there is a lack of information in the literature concerning its physiology.…”

Uptake of iron by Kluyveromyces marxianus DSM 5422 cultivated in a whey‐based medium

“…For example, the K S values of K. marxianus for glucose were found to be 0.065 g/L and 0.23 g/L . In contrast, the published K S values of K. marxianus for lactose extend over a wide range: 0.012 g/L , 1.2 g/L , 10.5 g/L , 16 g/L , 20 g/L ; most of these K S values are implausibly high, which could be explained by the unsuitable mode of determination (the adaptation of the model to the measured data via multiparameter variation). Here, the γ μ ( C S,L ) term does not describe the precise μ – C S,L relation, but acts as a trigger from full growth with sugar to no growth after sugar depletion.…”