A semi-continuous analyzer for the fluorimetric determination of atmospheric formaldehyde

Sousa, Eliane Teixeira; Oliveira, Fábio Santos de; Alves, Arnaldo Cardoso; Andrade, Jaílson Bittencourt de

doi:10.1590/s0103-50532009000200010

Cited by 8 publications

(3 citation statements)

References 27 publications

(52 reference statements)

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“…Fluoral-P is colorless, while the product of reaction with FA, 3,5-diacetyl-1,4-dihydrolutidine (DDL), is yellow and can be detected by a spectrophotometer at 420 nm ( Figure 7 B). As many others, this protocol was initially applied to FA monitoring in air samples [ 109 , 110 ]. Subsequently, this approach was used by Yue and colleagues to estimate FA content in brain tissue lysates [ 111 ].…”

Section: From In Vitro To In Cellulomentioning

confidence: 99%

Approaches to Formaldehyde Measurement: From Liquid Biological Samples to Cells and Organisms

Lipskerov

Sheshukova

Komarova

2022

IJMS

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Formaldehyde (FA) is the simplest aldehyde present both in the environment and in living organisms. FA is an extremely reactive compound capable of protein crosslinking and DNA damage. For a long time, FA was considered a “biochemical waste” and a by-product of normal cellular metabolism, but in recent decades the picture has changed. As a result, the need arose for novel instruments and approaches to monitor and measure not only environmental FA in water, cosmetics, and household products, but also in food, beverages and biological samples including cells and even organisms. Despite numerous protocols being developed for in vitro and in cellulo FA assessment, many of them have remained at the “proof-of-concept” stage. We analyze the suitability of different methods developed for non-biological objects, and present an overview of the recently developed approaches, including chemically-synthesized probes and genetically encoded FA-sensors for in cellulo and in vivo FA monitoring. We also discuss the prospects of classical methods such as chromatography and spectrophotometry, and how they have been adapted in response to the demand for precise, selective and highly sensitive evaluation of FA concentration fluctuations in biological samples. The main objectives of this review is to summarize data on the main approaches for FA content measurement in liquid biological samples, pointing out the advantages and disadvantages of each method; to report the progress in development of novel molecules suitable for application in living systems; and, finally, to discuss genetically encoded FA-sensors based on existing natural biological FA-responsive elements.

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Section: From In Vitro To In Cellulomentioning

confidence: 99%

Approaches to Formaldehyde Measurement: From Liquid Biological Samples to Cells and Organisms

Lipskerov

Sheshukova

Komarova

2022

IJMS

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“…299,300 A semi-continuous analyzer based on two concentric tubes was proposed for fluorimetric determination of atmospheric formaldehyde. 301 The air sample was continually pumped through the external tube and the analyte was collected in a central microporous PTFE tube filled with the Fluoral-P reagent. Under optimized conditions defined by the Box-Behnken design, the detection limit and coefficient of variation were estimated as 0.55 ng L -1 and 8.6%, respectively.…”

Section: Instrumentation and Automationmentioning

confidence: 99%

Analytical chemistry in Brazil: healthy and growing

Rocha

Pereira-Filho²,

Nóbrega³

2009

J. Braz. Chem. Soc.

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A química brasileira está crescendo rapidamente, sendo um dos principais alicerces para o contínuo desenvolvimento do país. Inspirados no 15º Encontro Nacional de Química Analítica, ENQA, com um número recorde de participantes e comunicações, decidiu-se revisar os recentes desenvolvimentos em química analítica no Brasil. Foram escolhidas as mesmas áreas de aplicação adotadas para a organização temática do ENQA e selecionados artigos publicados por autores brasileiros em 7 periódicos de ampla penetração na área. Os dois periódicos científicos editados pela Sociedade Brasileira de Química também foram pesquisados. Esta revisão não é exaustiva, porém os trabalhos selecionados após 2008 mostram uma significativa visão geral da área. Pode-se concluir que a química analítica brasileira está saudável, em crescimento e certamente atenderá as demandas do país como uma potência emergida.Chemical sciences in Brazil are growing fast and being one of the main supports for the continuous development of the country. Inspired on the 15 th Brazilian Meeting on Analytical Chemistry, ENQA, with a record number of participants and communications, we have decided to review recent developments in analytical chemistry in Brazil. We have chosen the same application areas used for the thematic organization of the ENQA and have searched papers published by Brazilian authors in 7 major and general journals in analytical chemistry. The two scientific journals published by the Brazilian Chemical Society were also searched. This review is not comprehensive, but the selected papers published after 2008 shown an impressive overview of the area. We may conclude that analytical chemistry is healthy and growing in Brazilian scenario and certainly will attend the demands of Brazil as an emerged power.

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“…17 Procedures based on fluorimetric or spectrophotometric detection using acetylacetone (2,4-pentadione) for chemical derivatization have been commonly used. 18 The acetylacetone method was developed by Nash 19 and based on Hantzsch reaction. Formaldehyde reacts with acetylacetone in presence of ammonium acetate leading to the formation of 3,5-diacetyl-1,4-dihydrolutidine (DDL).…”

Section: Introductionmentioning

confidence: 99%

A Multicommuted Flow System for Spectrophotometric Determination of Formaldehyde in Mushroom

Rocha¹,

Richter²,

Muñoz³

et al. 2018

J. Braz. Chem. Soc.

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Formaldehyde is a carcinogenic compound that was employed as fungicide in the past. Despite its disuse, some foods such as mushrooms naturally produce formaldehyde, showing the importance of its determination. In this work, a mechanized flow system based on solenoid valves is proposed for spectrophotometric formaldehyde determination in mushrooms. The analyte was extracted from fresh and dried mushrooms samples by steam distillation with deionized water in the presence of phosphoric acid. The determination was based on the reaction between formaldehyde and acetylacetone in presence of ammonium acetate (Hantzsch reaction), leading to the formation of 3,5-diacetyl-1,4-dihydrolutidine (DDL, λ max = 412 nm). Linear response from 0.2 to 7.0 mg L -1 was achieved, with detection limit estimated at 0.02 mg L -1 (99.7% confidence level). Coefficient of variation and sample throughput were 1.1% (n = 15) and 17 h -1 , respectively. Per determination, 11 mg of acetylacetone and 30 mg of ammonium acetate were consumed, generating 4.2 mL of waste. The procedure was applied to formaldehyde determination in fresh and dried shiitake, and fresh shimeji, and the results agreed with those attained by reference method at the 95% confidence level.

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A semi-continuous analyzer for the fluorimetric determination of atmospheric formaldehyde

Cited by 8 publications

References 27 publications

Approaches to Formaldehyde Measurement: From Liquid Biological Samples to Cells and Organisms

Approaches to Formaldehyde Measurement: From Liquid Biological Samples to Cells and Organisms

Analytical chemistry in Brazil: healthy and growing

A Multicommuted Flow System for Spectrophotometric Determination of Formaldehyde in Mushroom

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