A semi-automated multi-endpoint reactive oxygen species activity analyzer (SAMERA) for measuring the oxidative potential of ambient PM<sub>2.5</sub>aqueous extracts

Yu, Haoran; Puthussery, Joseph V.; Verma, Vishal

doi:10.1080/02786826.2019.1693492

Cited by 23 publications

(28 citation statements)

References 73 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The average OPex measured at Delhi in our study is approximately twice the ambient PM2.5 OPex reported by Vreeland et al 41 for a southern Indian city of Bangalore (0.79 ± 0.13 nmol/min/m 3 ) during October 2013, but within the range of the OPex [(0.09 -3.04 nmol/min/m 3 for PM10 42 and 1.3 -7.2 nmol/min/m 3 for PM2.5 16 extracted in a water + methanol (1:1) mixture] reported for a few northwest regions (Rajasthan and Punjab) in India. Despite a short duration of our sampling campaign, the range of OPex at Delhi was much wider than PM2.5 OPex reported in other cities of the world such as Chicago, USA (0.04 -1.28 nmol/min/m 3 ), 43 Atlanta, USA (0.1-1.5 nmol/min/m 3 ), 44 Paris, France (0.1 -0.36 nmol/min/m 3 ), 45 Rome, Italy (0.11 -0.34 nmol/min/m 3 ), 45 Athens, Greece (0.1 -0.36 nmol/min/m 3 ), 45 and Beijing, China (0.11 to 0.49 nmol/min/m 3 ). 46 The differences in the OPex at these sites are possibly due to differences in the intrinsic redox activities and ambient PM2.5 mass concentrations.…”

Section: Methodsmentioning

confidence: 74%

Real-Time Measurements of PM_2.5 Oxidative Potential Using a Dithiothreitol Assay in Delhi, India

Puthussery

Singh

Rai

et al. 2020

Environ. Sci. Technol. Lett.

Self Cite

View full text Add to dashboard Cite

The oxidative potential (OP) of ambient particulate matter (PM) is a commonly used metric to link the aerosol exposure to its adverse health effects. In this study, we report the first-ever real-time measurements of ambient PM2.5 OP based on a dithiothreitol (DTT) assay in Delhi, during a late winter season (February 2019). The chemical composition of PM was also measured using various collocated online instruments to identify the chemical components driving the PM2.5 OP. The hourly averaged OP during the entire campaign ranged from 0.49 -3.60 nmol/min/m 3 , with an average value of 1.57±0.7 nmol/min/m 3 . The secondary organic aerosols appear to be the major driver for the variability in the intrinsic OP of PM2.5. Although, the average PM1 mass concentration at Delhi was 13-times the average PM2.5 mass concentration reported in Illinois, USA in a similar study, it was not accompanied by a proportionate increase in the OP (average volume normalized DTT activity of PM2.5 was only 5 times of that reported in Illinois). These findings reveal substantial spatial heterogeneity in the redox properties of PM and highlight the importance of determining the PM chemical composition along with its mass concentrations for predicting the overall health impacts associated with aerosol exposure.

show abstract

Section: Methodsmentioning

confidence: 74%

Real-Time Measurements of PM_2.5 Oxidative Potential Using a Dithiothreitol Assay in Delhi, India

Puthussery

Singh

Rai

et al. 2020

Environ. Sci. Technol. Lett.

Self Cite

View full text Add to dashboard Cite

show abstract

“…•OH-TPT assays [124], and a multi endpoint ROS activity analyzer for DTT, •OH in DTT and AA, GSH, •OH in SFL [125]. Those advances contribute to scaling-up the research efforts and to design future monitoring applications.…”

Section: Op Environmental Monitoringmentioning

confidence: 99%

Airborne Aerosols and Human Health: Leapfrogging from Mass Concentration to Oxidative Potential

Molina

Manzano

et al. 2020

Atmosphere

View full text Add to dashboard Cite

The mass concentration of atmospheric particulate matter (PM) has been systematically used in epidemiological studies as an indicator of exposure to air pollutants, connecting PM concentrations with a wide variety of human health effects. However, these effects can be hardly explained by using one single parameter, especially because PM is formed by a complex mixture of chemicals. Current research has shown that many of these adverse health effects can be derived from the oxidative stress caused by the deposition of PM in the lungs. The oxidative potential (OP) of the PM, related to the presence of transition metals and organic compounds that can induce the production of reactive oxygen and nitrogen species (ROS/RNS), could be a parameter to evaluate these effects. Therefore, estimating the OP of atmospheric PM would allow us to evaluate and integrate the toxic potential of PM into a unique parameter, which is related to emission sources, size distribution and/or chemical composition. However, the association between PM and particle-induced toxicity is still largely unknown. In this commentary article, we analyze how this new paradigm could help to deal with some unanswered questions related to the impact of atmospheric PM over human health.

show abstract

“…OP activities of PM 2.5 extracts were analyzed using SAM-ERA. The setup and operation protocol of SAMERA have been discussed in detail in Yu et al (2020). Briefly, the analysis of all OP endpoints for each extract was conducted in two stages: SLF-based endpoints were analyzed first, while DTTbased assays were conducted in the second stage.…”

Section: Op Analysismentioning

confidence: 99%

“…Analyzing each PM sample for all of these chemical assays is also time-consuming. To address this concern, we have previously developed an automated OP analysis instrument named SAMERA -the Semi-Automated Multi-Endpoint ROS-activity Analyzer -that can measure the five most commonly used OP endpoints (i.e., the consumption rate of AA and GSH in SLF, OP AA , and OP GSH ; the consumption rate of DTT and OP DTT and the generation rate of q OH in SLF, DTT, OP OH−SLF , and OP OH−DTT ) for a PM extract in less than 3 h (Yu et al, 2020). Many of these acellular endpoints have been widely implemented by various researchers to assess the oxidative properties of PM.…”

Section: Introductionmentioning

confidence: 99%

Spatiotemporal variability in the oxidative potential of ambient fine particulate matter in the Midwestern United States

Puthussery

Wang

et al. 2021

Atmos. Chem. Phys.

Self Cite

View full text Add to dashboard Cite

Abstract. We assessed the oxidative potential (OP) of both water-soluble and methanol-soluble fractions of ambient fine particulate matter (PM2.5) in the Midwestern United States. A large set of PM2.5 samples (N=241) was collected from five sites set up in different environments, i.e., urban, rural, and roadside, in Illinois, Indiana, and Missouri during May 2018–May 2019. Five acellular OP endpoints, including the consumption rate of ascorbic acid and glutathione in a surrogate lung fluid (SLF) (OPAA and OPGSH, respectively), dithiothreitol (DTT) depletion rate (OPDTT), and ⚫OH generation rate in SLF and DTT (OPOH−SLF and OPOH−DTT, respectively), were measured for all PM2.5 samples. PM2.5 mass concentrations in the Midwestern US as obtained from these samples were spatially homogeneously distributed, while most OP endpoints showed significant spatiotemporal heterogeneity. Seasonally, higher activities occurred in summer for most OP endpoints for both water- and methanol-soluble extracts. Spatially, the roadside site showed the highest activities for most OP endpoints in the water-soluble extracts, while only occasional peaks were observed at urban sites in the methanol-soluble OP. Most OP endpoints showed similar spatiotemporal trends between mass- and volume-normalized activities across different sites and seasons. Comparisons between two solvents (i.e., water and methanol) showed that methanol-soluble OP generally had higher activity levels than corresponding water-soluble OP. Site-to-site comparisons of OP showed stronger correlations for methanol-soluble OP compared to water-soluble OP, indicating a better extraction of water-insoluble redox-active compounds from various emission sources into methanol. We found a weak correlation and inconsistent slope values between PM2.5 mass and most OP endpoints. Moreover, the poor to moderate intercorrelations among different OP endpoints indicate different mechanisms of OP represented by these endpoints and thus demonstrate the rationale for analyzing multiple acellular endpoints for a better and more comprehensive assessment of OP.

show abstract

A semi-automated multi-endpoint reactive oxygen species activity analyzer (SAMERA) for measuring the oxidative potential of ambient PM_2.5aqueous extracts

Cited by 23 publications

References 73 publications

Real-Time Measurements of PM_2.5 Oxidative Potential Using a Dithiothreitol Assay in Delhi, India

Real-Time Measurements of PM_2.5 Oxidative Potential Using a Dithiothreitol Assay in Delhi, India

Airborne Aerosols and Human Health: Leapfrogging from Mass Concentration to Oxidative Potential

Spatiotemporal variability in the oxidative potential of ambient fine particulate matter in the Midwestern United States

Contact Info

Product

Resources

About

A semi-automated multi-endpoint reactive oxygen species activity analyzer (SAMERA) for measuring the oxidative potential of ambient PM2.5aqueous extracts

Cited by 23 publications

References 73 publications

Real-Time Measurements of PM2.5 Oxidative Potential Using a Dithiothreitol Assay in Delhi, India

Real-Time Measurements of PM2.5 Oxidative Potential Using a Dithiothreitol Assay in Delhi, India

Airborne Aerosols and Human Health: Leapfrogging from Mass Concentration to Oxidative Potential

Spatiotemporal variability in the oxidative potential of ambient fine particulate matter in the Midwestern United States

Contact Info

Product

Resources

About

A semi-automated multi-endpoint reactive oxygen species activity analyzer (SAMERA) for measuring the oxidative potential of ambient PM_2.5aqueous extracts

Real-Time Measurements of PM_2.5 Oxidative Potential Using a Dithiothreitol Assay in Delhi, India

Real-Time Measurements of PM_2.5 Oxidative Potential Using a Dithiothreitol Assay in Delhi, India