A semi-automated determination of phospholipids

Kraml, M.

doi:10.1016/0009-8981(66)90235-x

Cited by 227 publications

(58 citation statements)

References 9 publications

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“…Ca, Cr, Cu, Fe, K, Mg, Mn, Na, and Zn in feeds were determined (#968.08) using an atomic absorption spectrometer (AOAC 1995). The P in feed was determined according to a Technicon autoanalyzer method N-4C (Kraml 1966). Total FA content and composition of lipid supplements, diets, and feces were determined according to the procedures of Joy et al (1997), as modified by Crocker et al (1998).…”

Section: Methodsmentioning

confidence: 99%

Impacts of feeding lipid supplements high in palmitic acid or stearic acid on performance of lactating dairy cows

Chamberlain

DePeters

2016

Journal of Applied Animal Research

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Effects of feeding lipid supplements high in free fatty acids (FAs) of palmitate (C16:0) or stearate (C18:0) plus C18:1 cis 9&10 on milk yield and composition, apparent whole-tract apparent digestibility of FA, and the FA composition of milk lipids were studied. Four lactating Holstein cows with ruminal cannulae were used in a 4 × 4 Latin square assignment of four dietary treatments. Lipid supplements were enriched in free fatty concentrations of either palmitic acid (P) or stearic acid (S). The total mixed-ration contained 20 g/kg of lipid supplement that consisted of varying proportions of P to S. Treatments were: 100:0 P:S (P), 0:100 P:S (S), and two mixtures including 66:34 P:S (PS) and 34:66 P:S (SP). Milk yield and dry matter intake were not affected by lipid supplement, but the concentration and yield of fat in milk increased with increasing C16:0 in the lipid supplement. Increasing the C16:0 concentration in the lipid supplement increased its concentration in milk lipids while increasing C18:0 in the lipid supplement increased C18:0 concentration in milk fat. Whole-tract apparent digestibility of neutral detergent fiber (NDF) did not differ with lipid supplement, but organic matter digestibility tended to decrease with increasing C18:0 in the lipid supplement. Whole-tract digestibility of total FAs decreased with the increasing proportion of C18:0 to C16:0 in the lipid supplement. Apparent digestibility of C16:0 and C18:0 was not different within dietary treatment. The FA composition of the lipid supplement impacted both whole-tract digestibility of FAs and FA composition of milk lipids. ARTICLE HISTORY

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Section: Methodsmentioning

confidence: 99%

Impacts of feeding lipid supplements high in palmitic acid or stearic acid on performance of lactating dairy cows

Chamberlain

DePeters

2016

Journal of Applied Animal Research

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“…Mongrel dogs, weighing [18][19][20][21][22][23][24][25][26][27] kg, were fed standard dog chow until 16 h before the study and had free access to water. Kidneys were removed from the dogs under Nembutal (Abbott Laboratories, North Chicago, Ill.) anesthesia.…”

Section: Methodsmentioning

confidence: 99%

Effects of Glucose and Parathyroid Hormone on the Renal Handling of Myoinositol by Isolated Perfused Dog Kidneys

Molitoris¹,

Hruska

Fishman

et al. 1979

J. Clin. Invest.

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A B S T R A C T The effects of glucose and parathyroid hormone (PTH) on the transport and metabolism of myoinositol (MI) and [2-3H] 1110 release were about coincidental with the increase in urinary cyclic 3',5'-AMP after PTH and preceded the peak phosphaturic effect of PTH. There was no detectable effect of PTH on MI synthesis from glucose as a source of the MI released into the urine and perfusate. However, PTH temporarily halted accumulation of tritiated MI catabolites. There was no effect of inactivated PTH on urinary cyclic 3',5'-AMP or on MI transport, which indicates that the PTH effect on MI handling was a specific hormonal effect. These studies clarify the renal metabolism of MI, and they demonstrate heretofore unknown effects of PTH on the renal handling and metabolism of MI. The effects of PTH on renal MI metabolism have important implications in renal carbohydrate metabolism and phospholipid turnover.

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“…Calcium was determined by the SMA 12/60 System Calcium method, which is a modification (Gitelman 1967) of the method of Kessler & Wolfman (1964). Phosphorus was determined by the SMA 12/60 System Inorganic Phosphate method, which is based on the formation of phosphomolybdic acid (Kraml 1966).…”

Section: Collagenmentioning

confidence: 99%

A Biochemical Analysis of Subchondral Bone of the Medial Tibial Condyle in the Normal State and in Osteoarthritis and Rheumatoid Arthritis

Lereim

Langeland

Romanus

et al. 1975

Acta Orthopaedica Scandinavica

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A semi-automated determination of phospholipids

Cited by 227 publications

References 9 publications

Impacts of feeding lipid supplements high in palmitic acid or stearic acid on performance of lactating dairy cows

Impacts of feeding lipid supplements high in palmitic acid or stearic acid on performance of lactating dairy cows

Effects of Glucose and Parathyroid Hormone on the Renal Handling of Myoinositol by Isolated Perfused Dog Kidneys

A Biochemical Analysis of Subchondral Bone of the Medial Tibial Condyle in the Normal State and in Osteoarthritis and Rheumatoid Arthritis

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