A SEM-study of a keratoconus and an artificially aged human cornea

Jongebloed, W.L.; Humalda, D.; Andel, Pek van; Worst, J.F.G.

doi:10.1007/bf00166694

Cited by 9 publications

(10 citation statements)

References 6 publications

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“…This is different from cells with a defective cell membrane, where almost no cell contents are left. On the endothelial surface, cells with a missing nucleus have been found as well (Jongebloed et al, 1986), as shown in Fig. 8.…”

Section: Resultsmentioning

confidence: 82%

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The keratoconus epithelium studied by SEM

Jongebloed

Worst²

1987

Doc Ophthalmol

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A keratoconus removed from an 18-year-old male was processed for SEM and examined with special attention for the epithelial surface. A broad degenerative band was found running in the vertical meridian, showing areas devoid of, or with only partially attached, epithelial cells, and also areas with abnormal cells either, with a defective cell membrane and without cell contents or with an intact cell membrane but without a cell nucleus. Stereoscopic images of these defective cells show that cell degeneration is sometimes found several cell layers deep into the epithelium.

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Section: Resultsmentioning

confidence: 82%

“…In the scanning electron microscope the rough epithelial surface is often given to be associated with a process of cell detachment and active cell regeneration (Jongebloed et al, 1986).…”

Section: Introductionmentioning

confidence: 99%

The keratoconus epithelium studied by SEM

Jongebloed

Worst²

1987

Doc Ophthalmol

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“…Specular microscopy studies have documented pleomorphism and polymegathism in keratoconus which has been attributed to CL wear [26][27][28][29]. An SEM analysis by Jongebloed et al [30,31] described endothelial degeneration in 2 ker atoconus cases. We undertook this study to further define endothelial cell changes of ker atoconus by studying the removed corneal buttons following penetrating keratoplasty (PKP).…”

Section: ]-mentioning

confidence: 99%

Pathology of Corneal Endothelium in Keratoconus

Sturbaum¹,

Peiffer²

1993

Ophthalmologica

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Keratoconus is characterized by stromal thinning and conical deformity of the cornea that affects a small but significant portion of the population. Although keratoconus has been well studied, endothehal changes have not been extensively investigated. We studied the endothelium of 14 keratoconus corneal buttons obtained over the past 6 years by penetrating keratoplasty using light microscopy, scanning electron microscopy and transmission electron microscopy. Observations were correlated with patient history. Corneas demonstrated: endothehal cell pleomorphism and polymegathism (6 corneas); endothehal cell degeneration (13), and evidence of anterior chamber inflammation (4). Patterns of endothehal damage were variable ranging from isolated cell membranolysis to denudement of Descemet’s membrane. Less damage was present at the apex of the cones than that observed in a circumferential pattern at the bases. In general the damage observed correlated with the severity and duration of the keratoconus with 9 years being the dividing time between mild and severe endothehal cell damage. These observations support other studies that implicate contact lens wear as a cause of pleomorphism and polymegathism in these patients. Endothehal cell alterations are likely a secondary event occurring due to mechanical stresses.

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“…25 & 26), the bordering corneal tissue shows cells with numerous microvilli which is indicative for a high state of activity [16]. The corneal endothelium close to the metal cylinder of the KP shows disrupture and repair of cells, collagenous bundles and other products of cell activity (Figs.…”

Section: Discussionmentioning

confidence: 99%

Glass-metal keratoprosthesis: Light and electron microscopical evaluation of experimental surgery on rabbit eyes

et al. 1989

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A keratoprosthesis (KP) is the last and only surgical resort to regain some visual acuity in eyes with severely damaged corneae. Corneal blindness represents an important percentage of the blind in the economically poor countries. Commercially available KP's, e.g. those made of PMMA, which are difficult to sterilize and vulnerable to surface damage, are too expensive in these countries. To overcome these disadvantages, we developed a new KP, made of a glass core melted into a platinum cylinder with flange. They were implanted unilaterally in eyes of ten Hollander rabbits intralamellarly. They were fixated by two stainless steel traction threads passed around the whole eyeball. We investigated this type of KP in the rabbit cornea, its acceptance by stroma, epi- and endothelium, and its hydro-mechanical dynamics in situ. No signs of infection or extrusion were observed. No epithelial downgrowth, nor adverse tissue reaction could be detected. LM and SEM showed endothelialization of the newly formed stroma around the central column of the KP. We conclude that this type of KP (although optically still to be optimized) has been accepted by the rabbit cornea and a clinical trial on cornea-blind patients is justified.

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A SEM-study of a keratoconus and an artificially aged human cornea

Cited by 9 publications

References 6 publications

The keratoconus epithelium studied by SEM

The keratoconus epithelium studied by SEM

Pathology of Corneal Endothelium in Keratoconus

Glass-metal keratoprosthesis: Light and electron microscopical evaluation of experimental surgery on rabbit eyes

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