A self-digitization chip integrated with hydration layer for low-cost and robust digital PCR

Ning, Yongfeng; Cui, Xu; Yang, Chao; Fu, Jing; Bian, Xiaojun; Yi, Lin; Li, Gang

doi:10.1016/j.aca.2018.12.029

Cited by 47 publications

(33 citation statements)

References 30 publications

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“…Many studies have demonstrated the tremendous application of using the combination of microfluidic chip and digital polymerase chain reaction (PCR). The reason is that compared to the commercial PCR assay, digital PCR technique on chips is more accurate, high throughput and less timeconsuming [91][92][93][94]. Digital PCR on microfluidic chips could be used to directly investigate the association among cancer ncRNA [95] and DNA methylation [96] from liquid biopsy.…”

Section: On-chip Ctdna and Ncrna Detectionmentioning

confidence: 99%

Multifunctional microfluidic chip for cancer diagnosis and treatment

Guo¹,

Zhang²,

Liu³

et al. 2021

Nanotheranostics

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Microfluidic chip is not a chip in the traditional sense. It is technologies that control fluids at the micro level. As a burgeoning biochip, microfluidic chips integrate multiple disciplines, including physiology, pathology, cell biology, biophysics, engineering mechanics, mechanical design, materials science, and so on. The application of microfluidic chip has shown tremendous promise in the field of cancer therapy in the past three decades. Various types of cell and tissue cultures, including 2D cell culture, 3D cell culture and tissue organoid culture could be performed on microfluidic chips. Patient-derived cancer cells and tissues can be cultured on microfluidic chips in a visible, controllable, and high-throughput manner, which greatly advances the process of personalized medicine. Moreover, the functionality of microfluidic chip is greatly expanding due to the customizable nature. In this review, we introduce its application in developing cancer preclinical models, detecting cancer biomarkers, screening anti-cancer drugs, exploring tumor heterogeneity and producing nano-drugs. We highlight the functions and recent development of microfluidic chip to provide references for advancing cancer diagnosis and treatment.

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Section: On-chip Ctdna and Ncrna Detectionmentioning

confidence: 99%

Multifunctional microfluidic chip for cancer diagnosis and treatment

Guo¹,

Zhang²,

Liu³

et al. 2021

Nanotheranostics

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“…Nowadays, there are two main ways. One is chamber-based, [6][7][8] and the other is droplet-based. [9][10][11] The chamberbased dPCR devices need to process tens of thousands of microreactors with the same volume on substrates of different materials (such as polydimethylsiloxane [PDMS], [12] polymethyl methacrylate [PMMA], [13] fused silica, [10] silicon, [14] etc.…”

Section: Doi: 101002/admi202001074mentioning

confidence: 99%

Off‐Chip Vertical Step Emulsification Droplets Preparation Device Applied for Droplet Digital PCR

Shi

Jiang

et al. 2020

Adv Materials Inter

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High efficient and stable droplets preparation is an essential technology in digital PCR (dPCR) and other analytical fields. In this paper, a microfluidic device called vertical step emulsification (VSE) device to produce emulsion droplets rapidly off‐chip is developed. VSE device prepares droplets by perfluoroalkoxy (PFA) capillary and microcentrifuge tube, when the continuous water phase in PFA capillary jets into the static oil phase in the microcentrifuge tube at a certain speed, the continuous water phase is emulsified into stable droplets due to the sudden change of surface tension. In order to control the droplet size accurately, a general screw mechanism to control the gap between the PFA capillary outlet and the bottom of the inner wall of microcentrifuge tube is used. Meanwhile, dPCR tests with high dynamic range throughput droplets from VSE device are performed. The results show that the off‐chip droplet preparation device developed by the authors not only gets rid of the complicated control structure, has the advantages of low cost, easy operation, and user‐friendly, but also provides a sustainable development scheme for the preparation of high‐throughput and stable droplets in a short time.

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“…The effect of inorganic additive Mg in the nucleation kinetics will also be investigated. In accordance with our previous design, 24,25 each device contains 10 000 cylindrical microcavities for holding the partitioned droplets, where these are connected by microchannels which allow transport of solutions. These features have the view; (b) Microcavity array for crystallization, the top view and the sectional view of the device.…”

Section: Introductionmentioning

confidence: 99%

Investigating the Nucleation Kinetics of Calcium Carbonate Using a Zero-Water-Loss Microfluidic Chip

Zhang

Gao

Meldrum

et al. 2020

Crystal Growth & Design

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Characterization of nucleation processes is essential to the development of strategies to control crystallization in many industrial, atmospheric, biological, and geological environments. An effective route to studying nucleation processes is the use of large numbers of small, identical volumes, where these minimize the effects of impurities that can dominate in bulk solution and allow the stochastic nature of nucleation to be considered. Here, we present a multilayered microfluidic device for nucleation studies that can be used to carry out 10 000 simultaneous reactions in identical microcavities, and 500 of them are used for investigating nucleation kinetics. Unlike droplet-based systems, no surfactants are required, and the presence of an integrated water

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A self-digitization chip integrated with hydration layer for low-cost and robust digital PCR

Cited by 47 publications

References 30 publications

Multifunctional microfluidic chip for cancer diagnosis and treatment

Multifunctional microfluidic chip for cancer diagnosis and treatment

Off‐Chip Vertical Step Emulsification Droplets Preparation Device Applied for Droplet Digital PCR

Investigating the Nucleation Kinetics of Calcium Carbonate Using a Zero-Water-Loss Microfluidic Chip

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