A Self-Catalytic Carbon Paste Electrode for the Detection of Vitamin B₁₂

Abstract

A simple, sensitive, and selective method for the detection of vitamin B12 (cyanocobalamin) is presented. A carbon paste electrode has been constructed with trans-1,2-dibromocyclohexane (DBCH) acting as both the paste binder and a reactive material facilitating the electrocatalytic detection of the target. The latter reaction is based on the electroreduction of the Co(III) center in vitamin B12 to Co(I), which specifically reacts with DBCH to produce electrocatalytic currents with the regeneration of Co (II); a detection limit of 8.5 x 10(-10) mol dm(-3) (based on 3sigma) was found. The electrode has been successfully used for the quantification of vitamin B12 in pharmaceutical products and biological matrix media.

Development of an electrochemical DNA biosensor for the detection of vitamin B12 (cyanocobalamin) at a carbon paste modified electrode with a manganese(II) complex

“…Furthermore, the calculated limit of detection of the proposed methodology is lower compared to those reported on references [4,[28][29][30][31]. In addition, the detection limit of the proposed methodology, comprising the modified CPE with (A) and dsDNA, is lower than that of the unmodified CPE [27]. On the other hand, the reported detection limits on references [27], [28], and [32] are lower that of the proposed biosensor.…”

“…In addition, the detection limit of the proposed methodology, comprising the modified CPE with (A) and dsDNA, is lower than that of the unmodified CPE [27]. On the other hand, the reported detection limits on references [27], [28], and [32] are lower that of the proposed biosensor. In spite of their advantages arising from low detection limits, generally modified electrodes are made of expensive materials like gold nanotubes [31] or screen printed electrodes [32].…”

“…On the other hand, voltammetric methods are highly sensitive and rather accurate, do not require expensive instrumentation, and are rather frequently used to determine group B vitamins and therefore B 12 . Electrochemical techniques like cyclic voltammetry (CV), differential pulse voltammetry (DPV), square wave voltammetry (SWV) and amperometry have been employed to the determination of B 12 [24][25][26][27][28][29][30][31][32][33][34]. Furthermore, chemical modifiers of electrode surfaces, particularly manganese complexes, give to the newly resulted electrodes excellent properties and can be utilized to the detection of B 12 [35][36][37][38][39].…”

Electrochemical Sensors

“…95 Detection limits of 1 nM were achieved with carbon paste electrodes using 1,2-dibromocyclohexane both as binder and electrocatalytic material. The electrode surfaces needed to be renewed after each measurement.…”

Organo-modified layered double hydroxide-catalyzed Fenton-like ultra-weak chemiluminescence for specific sensing of vitamin B12 in egg yolks

“…The classical analytical techniques for quantifying vitamin B 12 currently include microbiological [6], spectrophotometric [7], fluorescent [8], inductively coupled plasma mass spectrometry (ICP-MS) [9], and electrochemical methods [10]. Nowadays, a chemiluminescence (CL) technique for the determination of vitamin B 12 is becoming a promising technique with reliability, fast response, cheap instrument, simple operation and high sensitivity [11][12][13][14][15].…”