A self-assembling peptide hydrogel for ultrarapid 3D bioassays

Gagni, Paola; Romanato, Alessandro; Bergamaschi, Greta; Bettotti, Paolo; Vanna, Renzo; Piotto, Chiara; Morasso, Carlo; Chiari, Marcella; Cretich, Marina; Gori, Alessandro

doi:10.1039/c8na00158h

Cited by 21 publications

(28 citation statements)

References 61 publications

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“…In addition, laborious strategies may be required for hydrogel chip fabrication due to the viscosity of common hydrogels. We tackled these limitations by developing a nanostructured self-assembling peptide hydrogel (YF-Q11) characterized by favorable properties for 3D microarrays [57]. These include both low viscosity, enabling its direct use during automated spotting, combined with good mechanical robustness and, most relevantly, unimpaired and tunable diffusion properties of different macromolecules (peptides, proteins, antibodies).…”

Section: The Importance Of Probe Presentation: Conformation and Sumentioning

confidence: 99%

Peptides for Infectious Diseases: From Probe Design to Diagnostic Microarrays

et al. 2019

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Peptides and peptidomimetics have attracted revived interest regarding their applications in chemical biology over the last few years. Their chemical versatility, synthetic accessibility and the ease of storage and management compared to full proteins have made peptides particularly interesting in diagnostic applications, where they proved to efficiently recapitulate the molecular recognition properties of larger protein antigens, and were proven to be able to capture antibodies circulating in the plasma and serum of patients previously exposed to bacterial or viral infections. Here, we describe the development, integration and application of strategies for computational prediction and design, advanced chemical synthesis, and diagnostic deployment in multiplexed assays of peptide-based materials which are able to bind antibodies of diagnostic as well as therapeutic interest. By presenting successful applications of such an integrated strategy, we argue that they will have an ever-increasing role in both basic and clinical realms of research, where important advances can be expected in the next few years.

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Section: The Importance Of Probe Presentation: Conformation and Sumentioning

confidence: 99%

Peptides for Infectious Diseases: From Probe Design to Diagnostic Microarrays

et al. 2019

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“…[ 33,34 ] Hydrogel microarrays have also been implemented as a high‐throughput platform for cellular and molecular screening. [ 35,36 ]…”

Section: Introductionmentioning

confidence: 99%

“…[33,34] Hydrogel microarrays have also been implemented as a high-throughput platform for cellular and molecular screening. [35,36] Inspired by the high versatility and controllability of hydrogels, this study aims to develop hydrogel microlasers and to explore their fundamental characteristics in biolasing. Figure 1a shows the concept of a hydrogel biolaser in which precursor solutions mixed with fluorescent dyes are printed on a highly reflective dielectric mirror to form a cap-like cavity.…”

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Hydrogel Microlasers for Versatile Biomolecular Analysis Based on a Lasing Microarray

Gong

Qiao

Guan

et al. 2020

Advanced Photonics Research

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Biological microlasers have advanced rapidly in recent years, demonstrating their immense potential to use lasing emission as a sensing signal for monitoring molecular interactions. [1-6] Such lasers are known for their distinct advantages in terms of signal amplification, narrow linewidth, and strong intensity, which lead to unprecedented detection sensitivity of tiny changes in biological systems. [7-11] Most biolasers were realized through the introduction of fluorescent biomolecules (gain) into millimeter-sized resonators, resulting in difficulties for further applications. As such, whispering-gallery-mode (WGM) microlasers have come into play for their high Q factor, low mode volume, and miniaturized size. [12-15] A plethora of biocompatible materials have been used to form microlasers in recent years, including proteins, poly(lactic acid), starch, lipids, liquid crystals, cellulose, and polymers. [13,16-26] To move a step forward, it is essential to realize microlasers with the versatility to design, control, and functionalize different biostructures and molecules within the cavity. Hydrogels, one of the most popular biomaterials, have received tremendous attention for its high versatility among biocompatible materials over decades. [27,28] Endowed with unique 3D network structures and a porous interface, hydrogels enjoy priority in a wide range of applications in drug delivery, therapeutics, tissue engineering, and biosensing devices. [29-31] Hydrogels can be used to encapsulate

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“…The hydrogel creates a protective hydrate shell surrounding the proteins increasing their durability. Additionally, the one-step hydrogel based protein microarray fabrication provides a 3D matrix enabling a high density of the immobilized capture antibodies [12–14].…”

Section: Introductionmentioning

confidence: 99%

Hydrogel based protein biochip for parallel detection of biomarkers for diagnosis of a Systemic Inflammatory Response Syndrome (SIRS) in human serum

et al. 2019

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The Systemic Inflammatory Response Syndrome (SIRS), a sepsis related inflammatory state, is a self-defense mechanism against specific and nonspecific stimuli. The six most extensively studied inflammatory biomarkers for the clinical diagnosis of SIRS are interleukin 4 (hIL-4), interleukin 6 (hIL-6), interleukin 10 (hIL-10), tumor necrosis factor alpha (hTNF-α), interferon gamma (hIFN-γ) and procalcitonin (hPCT). These biomarkers are naturally present (but usually only at low concentration) in SIRS infected patients [1, 2] and thus the development of a highly sensitive detection method is of major clinical interest. However, the existing analytical techniques are lacking in required analytical sensitivity and parallel determination of these biomarkers. We developed a fast, easy and cost-efficient protein microarray biochip where the capture molecules are attached on hydrogel spots, enabling SIRS diagnosis by parallel detection of these six clinically relevant biomarkers with a sample volume of 25 μl. With our hydrogel based protein microarray biochip we achieved a limit of detection for hIL-4 of 75.2 pg/ml, for hIL-6 of 45.1 pg/ml, for hIL-10 of 71.5 pg/ml, for hTNF-α of 56.7 pg/ml, for IFN-γ of 46.4 pg/ml and for hPCT of 1.1 ng/ml in spiked human serum demonstrating sufficient sensitivity for clinical usage. Additionally, we demonstrated successful detection of two relevant SIRS biomarkers in clinical patient samples with a turnaround time of the complete analysis from sample-to-answer in less than 200 minutes.

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A self-assembling peptide hydrogel for ultrarapid 3D bioassays

Abstract: A peptide soft hydrogel is used as a 3D matrix for flash microarray bioassays.

Cited by 21 publications

References 61 publications

Peptides for Infectious Diseases: From Probe Design to Diagnostic Microarrays

Peptides for Infectious Diseases: From Probe Design to Diagnostic Microarrays

Hydrogel Microlasers for Versatile Biomolecular Analysis Based on a Lasing Microarray

Hydrogel based protein biochip for parallel detection of biomarkers for diagnosis of a Systemic Inflammatory Response Syndrome (SIRS) in human serum

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