A self-assembling amphiphilic peptide nanoparticle for the efficient entrapment of DNA cargoes up to 100 nucleotides in length

Tarvirdipour, Shabnam; Schoenenberger, Cora‐Ann; Benenson, Yaakov; Palivan, Cornelia G.

doi:10.1039/c9sm01990a

Cited by 17 publications

(25 citation statements)

References 87 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…We have demonstrated that KIW7 is able to promote cell surface association and partially deliver DNA with ~200 bp into HeLa cells, indicating the potential of this truncated version of Penetratin for the transport of nucleotide sequences larger than those successfully transported by CPPs in recent reports. 67,68 In fact, 200bp DNA duplexes internalized with assistance of KIW7 are about 10 times larger than microRNA and siRNA used to regulate cell metabolism and therefore KIW7 is potentially suitable for delivery of these species or even oligonucleotides.…”

Section: Discussionmentioning

confidence: 99%

Self-assembly and intracellular delivery of DNA by a truncated fragment derived from theTrojanpeptidePenetratin

et al. 2020

View full text Add to dashboard Cite

show abstract

Section: Discussionmentioning

confidence: 99%

Self-assembly and intracellular delivery of DNA by a truncated fragment derived from theTrojanpeptidePenetratin

et al. 2020

View full text Add to dashboard Cite

show abstract

“…Membrane active peptides interact with cellular membranes by traversing them, disrupting them or by residing at the membrane interface and fusing with them [19]. They are known to overcome site-specific delivery barriers and facilitate intracellular delivery of various bioactive cargos with low cytotoxicity [19,20]. Although there is a wide variety of membrane-active peptides, here we mainly discuss peptides for targeting nucleic acid delivery systems to specific cells and tissues, and peptides that assist in the delivery of nanocarriers across membrane barriers, such as cell penetrating peptides (CPPs), peptides facilitating endosomal escape and those that target nanocarriers to subcellular organelles (Figure 1).…”

Section: Peptide-guided Delivery Of Nucleic Acids Across Biological Barriersmentioning

confidence: 99%

Peptide-Assisted Nucleic Acid Delivery Systems on the Rise

Tarvirdipour

Skowicki

Schoenenberger

et al. 2021

IJMS

Self Cite

View full text Add to dashboard Cite

Concerns associated with nanocarriers’ therapeutic efficacy and side effects have led to the development of strategies to advance them into targeted and responsive delivery systems. Owing to their bioactivity and biocompatibility, peptides play a key role in these strategies and, thus, have been extensively studied in nanomedicine. Peptide-based nanocarriers, in particular, have burgeoned with advances in purely peptidic structures and in combinations of peptides, both native and modified, with polymers, lipids, and inorganic nanoparticles. In this review, we summarize advances on peptides promoting gene delivery systems. The efficacy of nucleic acid therapies largely depends on cell internalization and the delivery to subcellular organelles. Hence, the review focuses on nanocarriers where peptides are pivotal in ferrying nucleic acids to their site of action, with a special emphasis on peptides that assist anionic, water-soluble nucleic acids in crossing the membrane barriers they encounter on their way to efficient function. In a second part, we address how peptides advance nanoassembly delivery tools, such that they navigate delivery barriers and release their nucleic acid cargo at specific sites in a controlled fashion.

show abstract

“…The peptide-based micelles were non-cytotoxic at various concentrations and demonstrated superior delivery efficiency over common transfection agents, like Lipofectamine 2000 or Lipofectin ® . In another example, a rationally designed amphiphilic peptide that self-assembled into multicompartment micelles (MCMs) was able to efficiently entrap single- and double-stranded DNA up to 100 nucleotides in length ( Figure 2 A,B) [ 40 ]. These purely peptidic, nanosized MCMs demonstrated rapid and efficient uptake by cells ( Figure 2 C).…”

Section: Peptide-based Supramolecular Nanoassembliesmentioning

confidence: 99%

“…( B ) Normalized fluorescence correlation spectroscopy (FCS) autocorrelation curves for fluorescently labelled 100 nt ssDNA and 100 bp dsDNA (free and loaded in multi-compartment micelles). ( C ) Confocal laser scanning microscopy (CLSM) merged images (GFP and Atto550) of H2B-GFP expressing HeLa cells treated with 100 nt/bp ss/dsDNA-loaded (HR)3gT multi-compartment micelle nanoparticles after 24 h. Adapted and modified from [ 40 ], with permission. Copyright: 2020 Royal Society of Chemistry.…”

Section: Figurementioning

confidence: 99%

Peptide-Based Nanoassemblies in Gene Therapy and Diagnosis: Paving the Way for Clinical Application

et al. 2020

Self Cite

View full text Add to dashboard Cite

Nanotechnology approaches play an important role in developing novel and efficient carriers for biomedical applications. Peptides are particularly appealing to generate such nanocarriers because they can be rationally designed to serve as building blocks for self-assembling nanoscale structures with great potential as therapeutic or diagnostic delivery vehicles. In this review, we describe peptide-based nanoassemblies and highlight features that make them particularly attractive for the delivery of nucleic acids to host cells or improve the specificity and sensitivity of probes in diagnostic imaging. We outline the current state in the design of peptides and peptide-conjugates and the paradigms of their self-assembly into well-defined nanostructures, as well as the co-assembly of nucleic acids to form less structured nanoparticles. Various recent examples of engineered peptides and peptide-conjugates promoting self-assembly and providing the structures with wanted functionalities are presented. The advantages of peptides are not only their biocompatibility and biodegradability, but the possibility of sheer limitless combinations and modifications of amino acid residues to induce the assembly of modular, multiplexed delivery systems. Moreover, functions that nature encoded in peptides, such as their ability to target molecular recognition sites, can be emulated repeatedly in nanoassemblies. Finally, we present recent examples where self-assembled peptide-based assemblies with “smart” activity are used in vivo. Gene delivery and diagnostic imaging in mouse tumor models exemplify the great potential of peptide nanoassemblies for future clinical applications.

show abstract

A self-assembling amphiphilic peptide nanoparticle for the efficient entrapment of DNA cargoes up to 100 nucleotides in length

Abstract: To overcome the low efficiency and cytotoxicity associated with most non-viral DNA delivery systems we developed a purely peptidic self-assembling system that is able to entrap single- and double-stranded DNA of up to 100 nucleotides in length.

Cited by 17 publications

References 87 publications

Self-assembly and intracellular delivery of DNA by a truncated fragment derived from theTrojanpeptidePenetratin

Self-assembly and intracellular delivery of DNA by a truncated fragment derived from theTrojanpeptidePenetratin

Peptide-Assisted Nucleic Acid Delivery Systems on the Rise

Peptide-Based Nanoassemblies in Gene Therapy and Diagnosis: Paving the Way for Clinical Application

Contact Info

Product

Resources

About