A SELECTIVE METHOD FOR THE ISOLATION OF <i>HAEMOPHILUS</i> IN MATERIAL FROM THE RESPIRATORY TRACT

Hovig, B; Aandahl, Einar

doi:10.1111/j.1699-0463.1969.tb04510.x

Cited by 39 publications

(3 citation statements)

References 11 publications

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“…The results obtained for BAC are in agreement with those reported in the literature (Hovig and Aandahl 1969). The high resistance observed to GEN and BAC in both clinical isolates and reference strains encourage us to propose that GEN and BAC (at 0.25μg/ml) could be used to compose a selective medium for H. parasuis isolation in the laboratory.…”

Section: Discussionsupporting

confidence: 89%

Antimicrobial susceptibility patterns of Brazilian Haemophilus parasuis field isolates

et al. 2017

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Haemophilus parasuis is the etiological agent of Glässer’s disease (GD), an ubiquitous infection of swine characterized by systemic fibrinous polyserositis, polyarthritis and meningitis. Intensive use of antimicrobial agents in swine husbandries during the last years triggered the development of antibiotic resistances in bacterial pathogens. Thus, regular susceptibility testing is crucial to ensure efficacy of different antimicrobial agents to this porcine pathogen. In this study, 50 clinical isolates from South Brazilian pig herds were characterized and analyzed for their susceptibility to commonly used antibiotic. The identification and typing of clinical isolates was carried out by a modified indirect hemagglutination assay combined with a multiplex PCR. The susceptibility of each isolate was analyzed by broth microdilution method against a panel of 21 antimicrobial compounds. We found that field isolates are highly resistance to gentamycin, bacitracin, lincomycin and tiamulin, but sensitive to ampicillin, clindamycin, neomycin, penicillin, danofloxacin and enrofloxacin. Furthermore, an individual susceptibility analysis indicated that enrofloxacin is effective to treat clinical isolates with the exception of those classified as serovar 1. The results presented here firstly demonstrate the susceptibility of Brazilian clinical isolates of H. parasuis to antimicrobials widely used by swine veterinary practitioners and strengthen the need to perform susceptibility test prior to antibiotic therapy during GD outbreaks. In addition, because only six antimicrobial drugs (28.6%) were found effective against field isolates, a continuous surveillance of the susceptibility profile should be of major concern to the swine industry.

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Section: Discussionsupporting

confidence: 89%

Antimicrobial susceptibility patterns of Brazilian Haemophilus parasuis field isolates

et al. 2017

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“…Isolation of strains. Nasopharyngeal material taken with a cotton-swab on a curved metal rod was plated immediately on two different media 1 ) on 5 per cent horse blood agar plates, subsequently provided with a streak of a staphylococcus culture; and 2 ) on a selective medium consisting of chocolate agar (10 per cent heated bovine blood) 572 containing 300 pg/ml bacitracin as described by Hovig & Aandahl (1969). After incubation overnight at 35" C in an atmosphere supplemented with 10 per cent carbon dioxide the plates were examined for growth of Haemophilus species and other organisms of possible clinical interest.…”

Section: Bacteriological Examinationmentioning

confidence: 99%

Haemophilus in the Upper Respiratory Tract of Children

Kilian

Heine‐Jensen

Bülow

1972

Acta Pathologica Microbiologica Scandinavica Section B Microbio

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The occurrence of Haemophilus in nasopharyngeal material from 130 hospitalized children aged 0—7 years has been investigated. If a selective medium was used, the carrier rates were very close to 100 per cent. The lowest rate was found in children aged less than one year (78 per cent). Out of a total of 137 Haemophilus strains isolated, H. influenzae amounted to 49 per cent, the remaining 51 per cent were shared between the species H. parainfluenzae, H. haemolyticus and H. parahaemolyticus. V‐factor independent strains were rarely found. The methods used for the determination of the growth‐factor requirements and their importance for the classification of the bacterial strains are discussed. An unclarified but constant finding of antibodies to H. influenzae type d antigen in sera from children aged more than nine months is described. Such antibodies were not found in children below that age, and were rather infrequent in sera from a group of young adults. No correlation was found between the bacteriological findings, the clinical findings and the presence of antibodies to the six H. influenzae types in sera from the children investigated. Thus the investigation does not contribute to the understanding of the clinical significance of any of the Haemophilus species.

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“…πού ευνοεί τήν ανάπτυξη του βακτηριδίου, είναι 10 units/ml Sims, 1970). Οι Hovig-Aandahl (1969) συνιστούν τήν βακιτρακίνη σέ πυκνότητα 300 mg/1 . Tò αντιβιοτικό ενσωματώνεται στο υλικό καλλιέργειας ή χρησιμοποιούνται έτοιμα δισκία πού κυκλοφορούν στο εμπόριο.…”

Section: θρεπτικά υλικάunclassified

Αιμοφιλος Της Γριππης Στα Παιδια

Βογιατζη-Ηλιαδη¹

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A SELECTIVE METHOD FOR THE ISOLATION OF HAEMOPHILUS IN MATERIAL FROM THE RESPIRATORY TRACT

Cited by 39 publications

References 11 publications

Antimicrobial susceptibility patterns of Brazilian Haemophilus parasuis field isolates

Antimicrobial susceptibility patterns of Brazilian Haemophilus parasuis field isolates

Haemophilus in the Upper Respiratory Tract of Children

Αιμοφιλος Της Γριππης Στα Παιδια

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