A selective medium for the detection and enumeration of mesophilic sulphite-reducing clostridia in food monitoring programs

Eisgruber, H.; Reuter, G.

doi:10.1016/0963-9969(94)00040-f

Cited by 20 publications

(13 citation statements)

References 11 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…perfringens may be improved through simple modifications. Eisgruber and Reuter (1995) have reported improved recovery and selectivity for sulphite-reducing clostridia from food with Sulphite Cycloserine Azide agar, a modification of TSC, incubated at 37°C. Such media may have promise for the selective membrane filtration enumeration of Cl.…”

Section: Discussionmentioning

confidence: 99%

Evaluation of two media for the membrane filtration enumeration of Clostridium perfringens from water

Sartory¹,

Field²,

Curbishley³

et al. 1998

Lett Appl Microbiol

View full text Add to dashboard Cite

Two media (mCP medium and Tryptose Sulphite Cycloserine (TSC) agar) were evaluated for recovery of Clostridium perfringens in environmental and part‐treated drinking water. For laboratory strains of Clostridium, mCP was more selective and specific for Cl. perfringens than TSC, but was markedly less efficient for the enumeration of both vegetative cells and spores. For samples of river water and part‐treated drinking water, TSC recovered significantly greater numbers of Cl. perfringens than mCP. In contrast to previous reports, there was a significant number of false presumptive positive and negative isolates on mCP. TSC is a more suitable medium for the routine monitoring of water supplies for the presence of Cl. perfringens.

show abstract

Section: Discussionmentioning

confidence: 99%

Evaluation of two media for the membrane filtration enumeration of Clostridium perfringens from water

Sartory¹,

Field²,

Curbishley³

et al. 1998

Lett Appl Microbiol

View full text Add to dashboard Cite

show abstract

“…1984; Cato et al. 1986; Sartory 1986; Eisgruber and Reuter 1991, 1995; Sartory et al. 1993, 1998) or reported as being acid phosphatase positive (Ueno et al.…”

Section: Methodsmentioning

confidence: 99%

Evaluation of acid phosphatase as a confirmation test for Clostridium perfringens isolated from water

Sartory

Waldock

Davies

et al. 2006

Lett Appl Microbiol

View full text Add to dashboard Cite

Aims: To evaluate testing for acid phosphatase as an alternative method for the confirmation of Clostridium perfringens isolated from water. Methods and Results: Sixty‐two reference strains of Clostridium were tested for their ability to produce acid phosphatase, as well as reduction of sulfite on tryptose sulfite cycloserine agar (TSC) and production of fluorescence in TSC supplemented with 4‐methylumbelliferylphosphate (MUP). Additionally 155 environmental presumptive C. perfringens isolates from TSC incubated at 44°C were identified and tested for acid phosphatase production and by the conventional MNLG (testing for motility, nitrate reduction, lactose fermentation and gelatin liquefaction) confirmation procedure. Twenty‐seven strains from 15 species of Clostridium‐reduced sulfite to some extent on TSC incubated at 44°C, with a significant number of species being able to grow well at this temperature, indicating that a confirmation step is needed for the enumeration of C. perfringens on this medium. All 10 strains of C. perfringens tested, together with one strain each of Clostridium baratii and Clostridium rectum produced acid phosphatase. These also produced fluorescence on MUP supplemented TSC, as did 13 strains of acid phosphatase negative, sulfite‐reducing clostridia, representing nine species. Of the environmental isolates, 114 were identified as C. perfringens of which 108 (94·7%) were confirmed by the acid phosphatase test compared with 104 (91·2%) by the MNLG tests. Conclusions: Testing for acid phosphatase production is at least as reliable, and much simpler to perform, than the current standard confirmation MNLG procedure. Incorporation of MUP into TSC does not reliably improve the identification of presumptive C. perfringens. Significance and Impact of the Study: Application of testing for acid phosphatase as a confirmation test for C. perfringens would substantially simplify the analysis for this bacterium from water samples, and reduce the analysis time to confirmed counts.

show abstract

“…As C. perfringens was found in 20% of minced meat samples examined microbiologically according to the EU Directive 88/657/ECC [15], 111 C. perfringens isolates were analyzed by ribotyping in order to collect basic information for a contamination study. Isolates were obtained by the pour-plate technique with sul¢te-cycloserine-azide medium [16] and con-¢rmed by acid phosphatase and reverse-CAMP testing as described [17]. Ten C. perfringens food isolates from doner kebab, shrimp paste, crab paste, and ¢sh sauce`tai pla' were analyzed by ribotyping and PFGE ( Table 2).…”

Section: Strainsmentioning

confidence: 99%

Molecular methods for the analysis of Clostridium perfringens relevant to food hygiene

Schalch

1999

FEMS Immunology and Medical Microbiology

View full text Add to dashboard Cite

Clostridium perfringens continues to be a common cause of food-borne disease [1,2]. It produces an enterotoxin (CPE) which is released upon lysis of the vegetative cell during sporulation in the intestinal tract. Catering premises with insufficient cooling and reheating devices often seem to be the cause of outbreaks of C. perfringens food poisoning. Typing of C. perfringens is of great importance for investigating sources of food poisoning cases and for studying the epidemiology of this microorganism. This report describes the examination of 155 C. perfringens isolates by molecular methods. Isolates were taken from 10 food poisoning outbreaks and cases (n = 34, food and fecal isolates) and from meat and fish pastes (n = 121). Isolates were characterized by plasmid profiling, ribotyping, and/or macrorestriction analysis by pulsed-field gel electrophoresis (PFGE). Results show that all three methods are suitable for classifying C. perfringens isolates below the species level. Ribopatterns and PFGE patterns can be interpreted more easily than plasmid profiling results and can be recommended for contamination studies and epidemiologic investigation of food poisonings associated with C. perfringens. ß

show abstract

A selective medium for the detection and enumeration of mesophilic sulphite-reducing clostridia in food monitoring programs

Cited by 20 publications

References 11 publications

Evaluation of two media for the membrane filtration enumeration of Clostridium perfringens from water

Evaluation of two media for the membrane filtration enumeration of Clostridium perfringens from water

Evaluation of acid phosphatase as a confirmation test for Clostridium perfringens isolated from water

Molecular methods for the analysis of Clostridium perfringens relevant to food hygiene

Contact Info

Product

Resources

About