A selective fluorimetric method for the determination of some 1,4-benzodiazepine drugs containing a hydroxyl group at C-3

Walash, M. I.; Belal, F.; Metwally, Mohammed El‐Sayed; Hefnawy, Mohamed M.

doi:10.1016/0731-7085(94)00070-0

Cited by 18 publications

(4 citation statements)

References 10 publications

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“…These methods include spectrophotometry and spectrofluorimetry [2][3][4], potentiometry [5,6], indirect kinetic catalytic method by stopped flow technique [7], immunoassays [8], modified carbon-paste electrodes as sensors [9], but the vast majority of the published methods involve separation techniques, particularly HPLC [10][11][12][13][14][15][16], MEKC [17][18][19], LC-MS [20][21][22][23][24], with atmospheric pressure chemical ionization [25], ESI [26][27][28][29], thermospray ionization [30], negative ion chemical ionization [31], GC [32][33][34][35][36][37][38], GC-MS [39][40][41], and CE [28,42].…”

Section: Introductionmentioning

confidence: 99%

Development of a validated HPLC method for the determination of four 1,4‐benzodiazepines in human biological fluids

Samanidou

Pechlivanidou

Papadoyannis³

2007

J of Separation Science

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A simple and sensitive HPLC method was developed and validated for the determination of four frequently prescribed 1,4-benzodiazepines: alprazolam (ALP), bromazepam (BRZ), diazepam (DZP), and flunitrazepam (FNZ). Separation was achieved on an Inertsil C8 analytical (250 mm x 4 mm, 5 microm) column, after selective extraction of benzodiazepine drugs from biological matrices by means of SPE. Isocratic elution was performed with a mobile phase consisting of CH3COONH4, 0.05 M CH3OH, and CH3CN (33:57:10 by volume). Quantification was performed at 240 nm with mefenamic acid (6 ng/microL) as the internal standard. DSC-18 Supelco cartridges provided high absolute recoveries (81-115%). The developed method was fully validated in terms of selectivity, linearity, accuracy, precision, stability, and sensitivity. Repeatability (n = 8) and between-day precision (n = 8) revealed RSD <12%. Recoveries from biological samples ranged from 81.2 to 115%. The detection limit of the method was calculated as 3.3-10.2 ng in blood plasma and 2.6-12.6 ng in urine for 20 microL injection volume. The method was applied to spiked biological matrices. Moreover, the method was applied to real samples of urine after an oral administration.

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Section: Introductionmentioning

confidence: 99%

Development of a validated HPLC method for the determination of four 1,4‐benzodiazepines in human biological fluids

Samanidou

Pechlivanidou

Papadoyannis³

2007

J of Separation Science

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“…Although the native fluorescence of benzodiazepines is low (Gifford et al 1977), several fluorescent derivatization procedures allow fluorimetric detection (Valentour et al 1975;De Giovanni and Chiarotti 1988;Berrueta, Gallo, and Vicente 1993;Walash et al 1994;Dolej s sová et al 1999).…”

Section: Introductionmentioning

confidence: 99%

Study of On-Line Fluorimetric Determination of Oxazepam. Application to the Screening of 1,4-Benzodiazepines Giving Oxazepam as Metabolite in Urine

et al. 2013

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An automated, rapid, and sensitive flow injection method was developed for the fluorimetric determination of oxazepam and afterward applied for the screening of benzodiazepines with oxazepam as a common metabolite in urine. This method is based on the hydrolysis of oxazepam to the corresponding benzophenone and subsequent cyclization to form the fluorescent molecule 2-chloro-9(10H)-acridinone. Both reactions were allowed to proceed in a two channel flow injection manifold. The physical and chemical variables affecting the method were optimized and a linear calibration graph obtained. Oxazepam was detectable in the 40-700 ng mL À1 range. The detection limit of this method was 35 ng mL À1 for k ¼ 3 and the throughput was 25 samples h À1 . The method was successfully applied for the screening of oxazepam in urine samples collected at different times after the single oral administration of Valium and Tranxilium by two healthy volunteers.

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“…There are many reports of the determination of lorazepam in human biological material and dosage forms by HPLC [1 4], UV densitometry [5], selective fluorimetry [6], difference spectrophotometry [7], and catalytic thermometric methods [8], and of the analysis of the lorazepam-related compounds 2-amino-2',5-dichlorobenzophenone (compound B), 6-chloro-4-(o-chlor-ophenyl)-2-quinazolinecarboxaldehyde (compound C), and 6-chloro-4-(o-chlorophenyl)-2-quinazolinecarboxylic acid (compound D) by TLC [1,9]. Quality control of Lorazepam tablets requires the separation and determination of the active substance and related compounds.…”

Section: Introductionmentioning

confidence: 99%

HPLC determination of lorazepam and lorazepam-related compounds in pharmaceutical formulations

Orlovic¹,

Radulovic²,

Ivanović³

et al. 2000

Chromatographia

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A sensitive, accurate and reproducible high performance liquid chromatographic (HPLC) procedure has been developed for the separation and analysis of Iorazepam and related compounds. A 20-1xL sample was separated by reversed-phase HPLC on a 150 mm • 4.6 mm, 5-1~m particle, Wakosil column with 0.1 M ammonium acetate (pH adjusted to 6.0 with acetic acicl)-acetonitrile-methanol, 1:1:1 (v/v) as mobile phase.

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A selective fluorimetric method for the determination of some 1,4-benzodiazepine drugs containing a hydroxyl group at C-3

Cited by 18 publications

References 10 publications

Development of a validated HPLC method for the determination of four 1,4‐benzodiazepines in human biological fluids

Development of a validated HPLC method for the determination of four 1,4‐benzodiazepines in human biological fluids

Study of On-Line Fluorimetric Determination of Oxazepam. Application to the Screening of 1,4-Benzodiazepines Giving Oxazepam as Metabolite in Urine

HPLC determination of lorazepam and lorazepam-related compounds in pharmaceutical formulations

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