“…Thus it is specifically the activity of mitochondrial GPAT that is decreased in the diabetic-or starved-rat liver [11,12]. Moreover, insulin acutely increases mitochondrial GPAT activity in the perfused rat liver [13].…”
Section: Enzymes Involved In the Partitioning Of Fatty Acids Between mentioning
“…Thus it is specifically the activity of mitochondrial GPAT that is decreased in the diabetic-or starved-rat liver [11,12]. Moreover, insulin acutely increases mitochondrial GPAT activity in the perfused rat liver [13].…”
Section: Enzymes Involved In the Partitioning Of Fatty Acids Between mentioning
“…This was mainly due to a significant decrease in the NEM-insensitive activity rather than a change in the microsomal activity. It is noteworthy that in various other states, alterations in hepatic GPAT, that parallel changes in hepatic triacylglycerol synthesis are mainly confined to the mitochondrial form of the enzyme [4][5][6]. In the GSD(+) state there was also a significant decrease in the total hepatic DHAPAT activity (table 2).…”
Section: Resultsmentioning
confidence: 97%
“…Accordingly measurements have been made of hepatic glycerolphosphate acyltransferase (GPAT) activities (mitochondrial and microsomal) and dihydroxyacetonephosphate acyltransferase (DHAPAT) activity. These activities have been found to change in a number of physiological states and change in parallel with changes in hepatic triacylglycerol synthesis and secretion [4][5][6][7]. The activity of the overt form of carnitine pahnitoyltransferase (CPT1) has also been measured in liver mitochondria and the sensitivity of this enzyme to inhibition by malonyl CoA determined.…”
Section: Introduction 2 Materials and Methodsmentioning
“…GPAT and fatty acyl CoA synthetase assays were performed immediately on portions of whole homogenates. Methods for assay of Acyl CoA, L-glycerol 3 phosphate-o-acyltransferase (EC 2.3.1.15) (in the presence of 6 mg/ml fatty acid-poor albumin) and glutamate dehydrogenase (EC 1.4.1.2)are described [11]. Long chain fatty acyl CoA synthetase (EC 6.2.1.3) was assayed at 30°C by following the incorporation of [3H] coenzyme A into palmitoyl CoA as described [12].…”
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