A selective agar medium for isolation, enumeration and morphological identification of Fusarium proliferatum

Isack, Yochai; Benichis, Marina; Gillet, Daryl; Gamliel, A.

doi:10.1007/s12600-014-0392-7

Cited by 7 publications

(2 citation statements)

References 14 publications

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“…F. oxysporum f. sp. cucumerinum isolates were cultured on Komada’s Fusarium -selective medium ( Isack et al., 2014 ) and confirmed on the basis of microscopic studies of the shape and size of macro- and micro-conidia ( Booth, 1971 ). Analytically pure sodium silicate (Na 2 SiO 3 ·9H 2 O) was purchased from Sangon Biotech Co., Ltd. (Shanghai, China).…”

Section: Methodsmentioning

confidence: 99%

Silicon enhances plant resistance to Fusarium wilt by promoting antioxidant potential and photosynthetic capacity in cucumber (Cucumis sativus L.)

et al. 2022

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Fusarium wilt, caused by Fusarium oxysporum f. sp. cucumerinum (Fo), is a severe soil-borne disease affecting cucumber production worldwide, particularly under monocropping in greenhouses. Silicon (Si) plays an important role in improving the resistance of crops to Fusarium wilt, but the underlying mechanism is largely unclear. Here, an in vitro study showed that 3 mmol·l-1 Si had the best inhibitory effect on the mycelial growth of F. oxysporum in potato dextrose agar (PDA) culture for 7 days. Subsequently, the occurrence of cucumber wilt disease and its mechanisms were investigated upon treatments with exogenous silicon under soil culture. The plant height, stem diameter, root length, and root activity under Si+Fo treatment increased significantly by 39.53%, 94.87%, 74.32%, and 95.11% compared with Fo only. Importantly, the control efficiency of Si+Fo was 69.31% compared with that of Fo treatment. Compared with Fo, the activities of peroxidase (POD), catalase (CAT), and ascorbate peroxidase (APX) significantly increased by 148.92%, 26.47%, and 58.54%, while the contents of H2O2, O2·−, and malondialdehyde (MDA) notably decreased by 21.67%, 59.67%, and 38.701%, respectively, in roots of cucumber plants treated with Si + Fo. Compared with Fo treatment, the net photosynthesis rate (Pn), stomatal conductance (Gs), transpiration rate (Tr), maximum RuBisCO carboxylation rates (Vcmax), maximum RuBP regeneration rates (Jmax), and activities of ribulose-1,5-bisphosphate carboxylase (RuBisCO), fructose-1,6-bisphosphatase (FBPase), and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) and the expression of FBPA, TPI, SBPase, and FBPase in Si+Fo treatment increased significantly. Furthermore, Si alleviated stomatal closure and enhanced endogenous silicon content compared with only Fo inoculation. The study results suggest that exogenous silicon application improves cucumber resistance to Fusarium wilt by stimulating the antioxidant system, photosynthetic capacity, and stomatal movement in cucumber leaves. This study brings new insights into the potential of Si application in boosting cucumber resistance against Fusarium wilt with a bright prospect for Si use in cucumber production under greenhouse conditions.

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Section: Methodsmentioning

confidence: 99%

Silicon enhances plant resistance to Fusarium wilt by promoting antioxidant potential and photosynthetic capacity in cucumber (Cucumis sativus L.)

et al. 2022

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“…Fusarium proliferatum , a broadly distributed saprophytic pathogen, can cause destructive diseases to an extremely wide range of hosts that span several plant families, including maize [ 4 ], tomato [ 3 ], garlic [ 5 ] and soybean [ 6 ]. Additionally, F. proliferatum has been reported to produce a number of mycotoxins (including fusaric acid, fumonisin, fusaproliferin, beauvericin and moniliformin) that pose a serious threat to global food security and human health [ 7 , 8 , 9 , 10 ]. Fusarium proliferatum is an endophyte that dwells on the plant and produces a large number of conidia that can survive for many years in the soil [ 11 ].…”

Section: Introductionmentioning

confidence: 99%

Transcriptome Analysis of Tomato Leaf Spot Pathogen Fusarium proliferatum: De novo Assembly, Expression Profiling, and Identification of Candidate Effectors

Gao

Yao

Liu

et al. 2017

IJMS

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Leaf spot disease caused by the fungus Fusarium proliferatum (Matsushima) Nirenberg is a destructive disease of tomato plants in China. Typical symptoms of infected tomato plants are softened and wilted stems and leaves, leading to the eventual death of the entire plant. In this study, we resorted to transcriptional profile analysis to gain insight into the repertoire of effectors involved in F. proliferatum–tomato interactions. A total of 61,544,598 clean reads were de novo assembled to provide a F. proliferatum reference transcriptome. From these, 75,044 unigenes were obtained, with 19.46% of the unigenes being assigned to 276 Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways, with 22.3% having a homology with genes from F. fujikuroi. A total of 18,075 differentially expressed genes (DEGs) were identified, 720 of which were found to code for secreted proteins. Of these, 184 were identified as candidate effectors, while 79.89% had an upregulated expression. Moreover, 17 genes that were differentially expressed in RNA-seq studies were randomly selected for validation by quantitative real-time polymerase chain reaction (qRT–PCR). The study demonstrates that transcriptome analysis could be an effective method for identifying the repertoire of candidate effectors and may provide an invaluable resource for future functional analyses of F. proliferatum pathogenicity in F. proliferatum and tomato plant–host interactions.

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Diagnostic Tools for Plant Biosecurity

Thomas¹,

Wood²,

Gullino

et al. 2017

Practical Tools for Plant and Food Biosecurity

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A selective agar medium for isolation, enumeration and morphological identification of Fusarium proliferatum

Cited by 7 publications

References 14 publications

Silicon enhances plant resistance to Fusarium wilt by promoting antioxidant potential and photosynthetic capacity in cucumber (Cucumis sativus L.)

Silicon enhances plant resistance to Fusarium wilt by promoting antioxidant potential and photosynthetic capacity in cucumber (Cucumis sativus L.)

Transcriptome Analysis of Tomato Leaf Spot Pathogen Fusarium proliferatum: De novo Assembly, Expression Profiling, and Identification of Candidate Effectors

Diagnostic Tools for Plant Biosecurity

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