Background: The emergence of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) pandemic has created an unprecedented global health, economic, and social challenges, generating fear. Fear can cause a range of physical and psychological disorders including depression, anxiety, and panic. Leading to lower social isolation and adherence to security measures, consequently increasing infection risk. The objective was to validate an 11-item unidimensional fear scale to identify and assess the severity of the fear of SARS-CoV-2. Methods: This was a cross-sectional study conducted through an online survey questionnaire. The sample included the Brazilian general population, randomly recruited by social networking platforms, during the pandemic. Participants aged ≥18 were included. A self-applicable fear scale named as the SARS-CoV-2 Fear Scale (SCoV-2-FS), comprising 11 questions addressing the fear of SARS-CoV-2 infection in performing daily activities was validated. The reliability and validity of the SCoV-2-FS were psychometrically evaluated using reliability measures and confirmatory factor analysis (CFA). The sample comprised 1332 Brazilian participants. Results: Cronbach’s α was 0.9, indicating very good internal reliability. The results of the CFA showed that the unidimensional factor structure of the SCoV-2-FS fitted well with the data.Limitations: The sampling method could have introduced selection bias in terms of socio-demographics.Conclusion: The SCoV-2-FS is a valid and reliable tool with robust psychometric properties, useful for assessing the psychological impact of fear due to COVID-19. Clinical relevance: The development of a scale may assist in the development of measures to control mental health disorders resulting from fear during and after the pandemic.