A second mutation enhances resistance of a tobacco mutant to sulfonylurea herbicides

Creason, Gary L.; Chaleff, R. S.

doi:10.1007/bf00257843

Cited by 42 publications

(17 citation statements)

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“…We have developed a transgenic 305423 soybean that produces seeds with increased levels of monounsaturated fatty acid (oleic acid) and decreased levels of polyunsaturated fatty acids (linoleic acid and linolenic acid). S4) (Falco and Li, 2007), both analogous to modifications found in different natural als mutants that are herbicide tolerant (Creason and Chaleff, 1988;Lee et al, 1988). 305423 soybean was developed by the introduction of two constructs (Supplemental Fig.…”

Section: Discussionmentioning

confidence: 99%

Molecular Characterization, Compositional Analysis, and Germination Evaluation of a High‐Oleic Soybean Generated by the Suppression ofFAD2‐1Expression

et al. 2014

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Soybean [Glycine max (L.) Merr.] oil is one of the most consumed and highest produced vegetable oils. However, the high percentage of polyunsaturated fatty acids in soybean oil limits its stability and shelf life. Here we report the generation and characterization of a high oleic acid soybean event (305423 soybean) that has an elevated content of monounsaturated oleic acid and reduced presence of polyunsaturated linoleic acid and linolenic acid. This transgenic event was generated by the insertion of a soybean ω‐6 desaturase (FAD2) gene fragment (gm‐fad2‐1), resulting in the suppression of endogenous FAD2‐1 expression, and a modified version of the soybean acetolactate synthase (ALS) gene (gm‐hra), used as a selectable marker. Molecular characterization revealed that 305423 soybean harbors four DNA inserts containing multiple copies of complete and partial gm‐fad2‐1 gene cassettes and a single copy of the intact gm‐hra gene cassette. However, these DNA inserts seemed to be inherited together without segregation. The suppression of endogenous FAD2‐1 led to an increase of oleic acid (18:1) from 211 to 765 g kg–1 along with concurrent reduction of both linoleic acid (18:2) from 525 to 36.2 g kg–1 and linolenic acid (18:3) from 93.5 to 53.9 g kg–1 out of total fatty acyl groups. The 305423 soybean was also subjected to nutrient composition analyses and phenotypic and agronomic performance evaluations and found to be comparable to the control soybean except for the expected traits.

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Section: Discussionmentioning

confidence: 99%

Molecular Characterization, Compositional Analysis, and Germination Evaluation of a High‐Oleic Soybean Generated by the Suppression ofFAD2‐1Expression

et al. 2014

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“…(Haughn and Somerville, 1986; Sathasivan et al, 1991; Mourad et al, 1993), sugar beet ( Beta vulgaris L.) (Hart et al, 1992; Wright and Penner, 1998), canola ( Brassica napus L.) (Swanson et al, 1989), cotton ( Gossypium hirsutum L.), (Subramanian et al, 1990; Rajasekaran et al, 1996), soybean [ Glycine max (L.) Merr.] (Sebastian et al, 1989), tobacco ( Nicotiana tabacum L.) (Chaleff and Ray, 1984; Creason and Chaleff, 1988), and wheat (Newhouse et al, 1992; Pozniak and Hucl, 2004). Resistance in most of these cases is due to a form of AHAS that is less sensitive to herbicide inhibition.…”

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confidence: 99%

Physiological and Molecular Characterization of Mutation‐Derived Imidazolinone Resistance in Spring Wheat

et al. 2004

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While imidazolinone herbicides are an attractive alternative for weed control, spring wheat (Triticum aestivum L.) is susceptible to most of these herbicides. Three mutant alleles conferring resistance to the imidazolinone herbicides were previously identified in spring wheat following seed mutagenesis, but little is known about the physiological and molecular basis of resistance conferred by these alleles. On the basis of acetohydroxyacid synthase (AHAS; E.C. 4.1.3.18) assays, imazethapyr resistance in genotypes TealIMI 10A (AhasL‐D1), TealIMI 11A (AhasL‐B1), TealIMI 15A, and BW755 (AhasL‐D1) was due to a herbicide resistant form of AHAS. TealIMI 15A, which is homozygous for two resistance alleles, was the most resistant at the enzyme level, suggesting resistance in wheat is additive. Nucleotide sequence analysis of clones derived from susceptible cultivar CDC Teal and resistant lines indicated the presence of three genes coding for the catalytic subunit of AHAS. Using a collection of T. aestivum cv. Chinese Spring aneuploid and deletion lines, ahasL‐D1, ahasL‐B1, and ahasL‐A1 mapped to the long arm of chromosomes 6D, 6B, and 6A, respectively. Comparison of partial AhasL‐D1, and AhasL‐B1 deduced amino acid allele sequences with wild‐type sequences indicated resistance was due to a Ser‐Asn substitution near the carboxyl terminal of resulting AHAS catalytic subunits. This substitution was not present on the single isolated clone of AhasL‐A1 from TealIMI 15A. This is the first report of the molecular characterization of the AHAS gene family from wheat.

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“…The imidazolinone herbicides act by inhibiting acetohydroxyacid synthase (AHAS, also referred to as acetolactate synthase (ALS) EC 2.2.1.6, formerly EC 4.1.3.18), the first enzyme that catalyzes the biochemical synthesis of the branched chain amino acids valine, leucine and isoleucine (Shaner et al 1984;Singh 1999). These broad-spectrum herbicides are an attractive weed control option, as they are potent at low application rates and possess relatively low mammalian toxicity (Newhouse et al Abbreviations: AHAS, acetohydroxyacid synthase; DPM, days to physiological maturity; DSE, days to spike emergence; HTr, herbicide treatment; HWC, hand-weeded control; SHT, standard herbicide treatment Plants resistant to AHAS-inhibiting herbicides have been successfully produced using mutagenesis in Arabidopsis thaliana L. Heynh (Haughn and Sommerville 1986;Sathasivan et al 1991;Mourad et al 1993;Jander et al 2003), sugar beet (Beta vulgaris L.) (Hart et al 1992;Wright and Penner 1998), canola (Brassica napus L.) (Swanson et al 1989), cotton (Gossypium hirsutum L.) (Subramanian et al 1990;Rajasekaran et al 1996), soybean (Glycine max L.) (Sebastian et al 1989), and tobacco (Nicotiana tabacum L.) (Chaleff and Ray 1984;Creason and Chaleff 1988). Using seed mutagenesis, Newhouse et al (1992) were the first to identify mutant winter wheat lines with moderate levels of resistance to the imidazolinones.…”

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Field performance of imazamox-resistant spring wheat

Pozniak¹,

Holm²,

Hucl³

2004

Can. J. Plant Sci.

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. 2004. Field performance of imazamox-resistant spring wheat. Can. J. Plant Sci. 84: 1205-1211. The imidazolinone herbicides possess high biological potency at low application rates, and are an attractive alternative for weed control in spring wheat (Triticum aestivum L.). Four mutation-derived spring wheat lines resistant to the imidazolinone class of herbicides were evaluated in field trials to investigate the levels of resistance to imazamox. Imazamox was applied post-emergence at 0 g (hand-weeded control; HWC) 20 g, 40 g, 80 g and 160 g active ingredient (a.i.) ha -1 . A standard herbicide treatment (SHT; clodinafoppropargyl, bromoxynil and MCPA ester) representing a common herbicide application to spring wheat in western Canada was also included. The cultivar CDC Teal was included as a susceptible control. At 160 g ha -1 , imazamox reduced yield in lines with a single resistance gene (TealIMI 10A, TealIMI 11A, and BW755) by up to 43% and delayed maturity up to 13 d. TealIMI 15A, the only line evaluated with two resistance genes, was the most resistant of all lines, with no significant increases in days to spike emergence (DSE), days to physiological maturity (DPM), and no reduction in yield at the highest rate of imazamox. The susceptible control was killed within 2 wk of imazamox application, regardless of application rate. These results indicate that two resistance genes are preferable for ensuring satisfactory levels of resistance to the formulation of imazamox used in these studies.

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A second mutation enhances resistance of a tobacco mutant to sulfonylurea herbicides

Cited by 42 publications

References 10 publications

Molecular Characterization, Compositional Analysis, and Germination Evaluation of a High‐Oleic Soybean Generated by the Suppression ofFAD2‐1Expression

Molecular Characterization, Compositional Analysis, and Germination Evaluation of a High‐Oleic Soybean Generated by the Suppression ofFAD2‐1Expression

Physiological and Molecular Characterization of Mutation‐Derived Imidazolinone Resistance in Spring Wheat

Field performance of imazamox-resistant spring wheat

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