A Second Look at Fiber Type Differentiation in Porcine Skeletal Muscle

Beermann, D. H.; Cassens, R. G.; Hausman, Gary

doi:10.2527/jas1978.461125x

Cited by 82 publications

(67 citation statements)

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“…This is consistent with the fact that primary myotubes are innervated before 45 dg in the pig ST muscle (Beermann et al, 1978). However, other studies carried out in the chick and rat have demonstrated that the initial generation and distribution of primary myotubes and fiber type pattern is independent of innervation or contractile activity (Harris, 1981;McLennan, 1983;Miller et al, 1985;Crow and Stockdale, 1986;Condon et al, 1990b).…”

Section: Discussion Primary Myotubes Expressed Slow Mhc In the Deep Msupporting

confidence: 72%

“…Indeed, it is generally assumed that muscle fibers undergo a neurally independent preprogrammed sequence of myosin transitions towards the fast phenotype (Embryonic + Neonatal -+ Fast) unless they receive a neural signal which will switch the programme towards the accumulation and maintenance of slow myosin, while inhibiting fast myosin synthesis (Salmons and Henriksson, 1981;Jolesz and Sreter, 1981;Butler-Browne et al, 1982;Condon et al, 1990b;Gambke et al, 1983;. The neural mechanisms leading to the formation of the islets of type I fibers are not understood, we only know that it is not due to the collateral branching of axons to innervate adjacent fibers (Beermann et al, 1978). Since the piglet can immediately walk and run after birth, the dramatic increase in contractile activity after birth may also be part of the mechanism.…”

Section: A Subpopulation Of Secondary Fibers Starts To Express Slow Mmentioning

confidence: 99%

“…Several studies have been carried out to describe the ontogenesis of this fiber type distribution (Davies, 1972;Thurley, 1972;Ashmore et al, 1973;Swatland and Cassens, 1973;Beermann et al, 1978;Wigmore and Stickland, 1983;Handel and Stickland, 1987). Pig myogenesis as in many mammalian species is a biphasic phenomenon with the sequential formation of two generations of muscle fibers.…”

Section: Introductionmentioning

confidence: 99%

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Pattern of muscle fiber type formation in the pig

Lefaucheur¹,

Edom

Ecolan³

et al. 1995

Developmental Dynamics

139

120

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The aim of this study was to analyze the temporal sequence of expression of the myosin isoforms in the populations of muscle fibers in the pig and to bring more information on the origin of the strikingly different pattern of fiber composition and distribution between the deep medial red (oxido-glycolytic) and superficial white (glycolytic) portions of semitendinosus (ST) muscle. Muscle samples were taken from 49-, 55-, 75-, 90-, 103-, and 113-(birth) day-old fetuses, from 6-, 11-, 21-, 35-, 50-, and 80-day-old piglets, and from a 3-year-old pig. Our results confirm the sequential formation of primary and secondary generation fibers. The use of immunohistochemistry and heterologous monoclonal antibodies (mAb) directed against specific myosin heavy chain (MHC) isoforms revealed a different pattern of gene expression between the two portions of the ST muscle for both generations of fibers. By 75 days of gestation (dg), primary myotubes from the deep medial portion stained positively for the anti-slow MHC mAb and negatively for the adult anti-fast MHC, whereas the opposite was observed in the superficial portion. Secondary fibers never expressed slow MHC until late gestation. Instead, they expressed an adult fast MHC isoform as soon as they formed in the deep medial portion and later on in the superficial portion. From late gestation to the first 3 postnatal weeks, slow MHC began to be expressed in a subpopulation of secondary fibers. These fibers were in the direct vicinity of primary myotubes in the deep medial portion, whereas their location could not be established in the superficial portion. The remaining secondary fibers matured to type IIA in the direct vicinity of these type I fibers and to type IIB at the periphery of the islets. In both portions of the muscle, a subpopulation of secondary fibers, the first ones to express slow MHC, also transitorily expressed a MHC that was identical or closely related to the a-cardiac MHC during the early postnatal period. A third generation of small diameter fibers was observed shortly after birth and reacted with the anti-fetal MHC mAb; their destiny remains to be established. The present work reveals a remarkable pattern of MHC gene expression in the pig and raises many questions on the real nature of these isoforms. In order to answer these questions, we have undertaken to make a cDNA library of pig skeletal muscle and to screen 0 1995 WILEY-LISS, INC.this library with the same mAbs used in the present study. o 1995 Wiley-Liss, Inc.

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Section: Discussion Primary Myotubes Expressed Slow Mhc In the Deep Msupporting

confidence: 72%

Section: A Subpopulation Of Secondary Fibers Starts To Express Slow Mmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Pattern of muscle fiber type formation in the pig

Lefaucheur¹,

Edom

Ecolan³

et al. 1995

Developmental Dynamics

139

120

View full text Add to dashboard Cite

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“…On the basis of the mATPase reaction, an increase of the SO type proportion was observed during the second half of the gestation and until the age of 16 weeks after birth (Swatland, 1975;Beermann et al, 1978;Szentuki and Cassens, 1979;Suzuki and Cassens, 1980). Using the oxidative enzyme activities as a criterion (eg, succinate dehydrogenase, SDH), an increase of the proportion of fibres with low oxidative capacity (ie FG type) was ascertained during postnatal growth (Cooper et al, 1970;Ashmore et al, 1972;Van Den Hende et al, 1972;Rehfeldt ef al, 1987).…”

mentioning

confidence: 99%

Fibre type differentiation during postnatal development of miniature pig skeletal muscles

Horák

1995

Reprod. Nutr. Dev.

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Summary ― Histochemical differentiation of 12 skeletal muscles with a different fibre type composition was studied in miniature pigs from 80 d of gestation to 1 year of age. Two fetal myofibre types were distinguished at 100 d of gestation by the mATPase reaction after acid preincubation. The staining for oxidative enzyme activities showed no conspicuous differences between fibres up to the 6th day after birth. Starting from this age it was possible to distinguish 3 fibre categories: SO (slow-twitch oxidative); FOG (fast-twitch oxidative-glycolytic); and FG (fast-twitch glycolytic). A characteristic cluster distribution of the 3 fibre types was observed in all studied muscles with the exception of the masseter muscle which consisted only of the type SO and FOG fibres with a mosaic arrangement. The

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“…During the latter part of prenatal life, muscle fibre types transform (Beermann et al 1978;Robelin et al 1991;Maier et al 1992), and the proportion of slow myofibres generally declines when myofibre number increases rapidly following commencement of secondary myogenesis. The proportion of each myofibre type within muscles begins to change towards the adult pattern as birth approaches (Sivachelvan and Davies 1981) although this change may be delayed until closer to birth in severely growth-retarded fetuses (Greenwood et al 1999a).…”

Section: Musclementioning

confidence: 99%

Nutritional and developmental effects on the intrinsic properties of muscles as they relate to the eating quality of beef

Oddy¹,

Harper²,

Greenwood³

et al. 2001

Aust. J. Exp. Agric.

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Abstract. The intrinsic properties (those extant at the time of slaughter) of bovine skeletal muscle as they relate to the subsequent quality attributes of beef are reviewed here. Attributes of bovine skeletal muscle that ultimately affect toughness, colour, fat content, flavour, juiciness, and nutritive value of beef are discussed. The dynamic nature of muscle development, particularly with regard to structure and composition, is highlighted. Variation in development of muscle structure and composition due to inherited (genetic) factors and environment (particularly nutrient supply) are described. Examples are given of the implications of sources of variation due to animal genotype, age, nutrient supply, and hormonal environment on muscle cellularity and growth, fibre type, connective tissue composition and structure as they affect meat quality attributes.Key intrinsic properties of muscle include muscle type, cellularity, size, myofibre type, connective tissue composition and structure, glycogen and fat content and proteolytic activity. Activity of the calpain system at slaughter is seen as an important attribute. Regulation of myofibrillar and connective tissue proteolysis in vivo are discussed together with implications for subsequent meat quality. Amongst the on-farm environmental factors, nutritional history and developmental pathway are identified as factors that can be responsible for significant variation in the intrinsic properties of muscle that contribute to variation in toughness, colour and fat content, and thus consumer liking of beef.

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A Second Look at Fiber Type Differentiation in Porcine Skeletal Muscle

Cited by 82 publications

References 0 publications

Pattern of muscle fiber type formation in the pig

Pattern of muscle fiber type formation in the pig

Fibre type differentiation during postnatal development of miniature pig skeletal muscles

Nutritional and developmental effects on the intrinsic properties of muscles as they relate to the eating quality of beef

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