A second capsidless hadakavirus strain with 10 positive-sense single-stranded RNA genomic segments from Fusarium nygamai

Abstract

Sequence data availability. The HadV1-1NL complete nucleotide genomic sequence was deposited in the GenBank/ENA/DDBJ database under accession numbers LC592214 to LC592223.

Mycovirus Hunting Revealed the Presence of Diverse Viruses in a Single Isolate of the Phytopathogenic Fungus Diplodia seriata From Pakistan

“…NGS sequencing provided many virus-like sequence contigs. Because the NGS data were from three fungal strains (see Materials and Methods), sequence contigs associated with previously reported mycoviruses derived from two other fungi ( Khan et al., 2021a ; Khan et al., 2021b ) were eliminated from the current analyses. RT-PCR analyses assigned a total of 17 virus-like sequence contigs to strain L3.…”

“…The fungal strain L3 ( D. seriata ) was collected and identified during a mycoviral screening survey conducted in 2018 from a diseased leaf of the Lokath plant ( Khan et al., 2021b ). Some mycoviruses identified as a result of this screening survey have already been published ( Khan et al., 2021a ; Khan et al., 2021b ; Khan et al., 2022 ). For isolating purified cultures of L3, surface sterilization of leaves was performed with 1% sodium hypochlorite solution and further washing was done using autoclaved sterile distilled water.…”

“…Next-Generation Sequencing of Viral RNA Total RNA fractions were separately obtained from three dsRNApositive fungal isolates, L3 (D. seriata, a phytopathogen), SP1 (F. mangiferae, a phytopathogen) and HK (Geotrichum candidum, a soil-inhabitant) and were pooled together as already described by Khan et al (Khan et al, 2021a;Khan et al, 2021b). Ribosomal RNA depletion, cDNA library construction and RNA sequencing were performed by Macrogen Inc. (Tokyo, Japan) as already described by Khan et al (Khan et al, 2021b).…”

“…How these co-infecting mycoviruses interplay remains unknown in these cases. We also have screened many different Pakistani fungal strains from different sources for mycovirus hunting and have reported molecular characterization of some of the discovered novel mycoviruses ( Khan et al., 2021a ; Khan et al., 2022 ). The tested fungal strains included the SP1 strain of Fusarium mangiferae that was found to be co-infected with 11 mycoviruses ( Khan et al., 2021b ).…”

Assessment of mycoviral diversity in Pakistani fungal isolates revealed infection by 11 novel viruses of a single strain of Fusarium mangiferae isolate SP1

“…The dsRNA patterns of 16 fungal isolates out of 36 dsRNA-positive ones are shown in , many of which show multiple bands. Several previously characterized fungal strains infected by RNA viruses were included in the dsRNA analysis as references, which included a botybirnavirus (AaBbV1)-infected Alternaria alternata isolate 4 a [34], a hadakavirus (HadV1-7n)-infected Fusarium oxysporum isolate 7 n and a HadV1-1NL infected Fusarium nygami isolate 1 NL [17, 35] (Fig. S2).…”