A search for virulence genes of Haemophilus parasuis using differential display RT-PCR

Hill, Claire; Metcalf, Devon; MacInnes, Janet I.

doi:10.1016/s0378-1135(03)00212-8

Cited by 50 publications

(33 citation statements)

References 22 publications

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“…Two main MEE groups were defined, but the relationship between the site of isolation and MEE profile was not found. Hill et al (2003) investigated virulence of the strain of H. parasuis 1185 (serovar 5) using differential display RT-PCR. Expression of seven genes was identified in a culture grown at 40°C to mimic conditions during acute disease.…”

Section: Virulence and Virulence Factorsmentioning

confidence: 99%

Haemophilus parasuis and Glässer's disease in pigs: a review

Nedbalcová¹,

Satran²,

Jaglič³

et al. 2006

Vet. Med.

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ABSTRACT:Haemophilus parasuis is a common epiphyte of the upper respiratory tract of pigs. The factors of H. parasuis pathogenicity that enable some strains to be virulent and consequently cause a clinical disease have not been established yet. Fifteen serovars of H. parasuis have been described at present. Individual serovars differ in virulence, and considerable differences in virulence also exist within each serovar. Virulent strains can particularly participate as microorganisms secondary to pneumonia, cause septicaemia without polyserositis or Glässer's disease characterized by polyserositis, pericarditis, arthritis and meningitis. Clinical symptoms of this disease are highly variable. Therefore, culture detection of causative agent, particularly from the brain, joints and polyserositis is an essential diagnostic tool. The disease caused by H. parasuis can be treated with antibiotics; however, oral or parenteral administration of very high doses of antibiotics is necessary. The level of animal hygiene and animal husbandry are important factors for prevention of this disease. Commercial or autogenous vaccines can be used in the immunoprophylaxis of pre-parturient sows and their progeny after weaning. For the production of autogenous vaccines, it is most effective to use isolates from animals with lesions present in CNS. Isolates recovered from arthritic and systemic sites of infection are less suitable and isolates recovered from lungs are not suitable at all because of their heterogeneity.

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Section: Virulence and Virulence Factorsmentioning

confidence: 99%

Haemophilus parasuis and Glässer's disease in pigs: a review

Nedbalcová¹,

Satran²,

Jaglič³

et al. 2006

Vet. Med.

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“…parasuis is the causative agent of Glässer's disease, porcine polyserositis and arthritis, which is a significant disease in the pork industry worldwide (Hill et al, 2003;Oliveira et al, 2003). It is believed that stress factors, such as transport, unfavourable environment and management conditions, are involved in H. parasuis breaching nasopharyngeal barriers and infecting systemically (Nicolet, 1986).…”

Section: Introductionmentioning

confidence: 99%

“…Of the 15 known serovars, serovars 1, 5, 10, 12, 13, and 14 have been shown to be highly virulent causing death or moribundity within four days, serovars 2, 4, and 15 were moderately virulent causing polyserositis, but not death, serovar 8 was considered to be of mild virulence resulting in mild symptoms and lesions, while serovars 3, 6, 7, 9, and 11 were avirulent causing no clinical symptoms or lesions (Kielstein and RappGabrielson, 1992). However, research to establish virulence markers and link outer membrane proteins and DNA profiles with virulence is still inconclusive (Ruiz et al, 2001;Hill et al, 2003).…”

Section: Introductionmentioning

confidence: 99%

Comparison of the indirect haemagglutination and gel diffusion test for serotyping Haemophilus parasuis

Turni¹

2005

Veterinary Microbiology

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The aim of this study was to compare the use of indirect haemagglutination (IHA) and gel diffusion (GD) tests for serotyping Haemophilus parasuis by the Kielstein-RappGabrielson scheme. All 15 serovar reference strains, 72 Australian field isolates, nine Chinese field isolates, and seven isolates from seven experimentally infected pigs were evaluated with both tests. With the IHA test, 14 of the 15 reference strains were correctly serotyped -with serovar 10 failing to give a titre with serovar 10 antiserum. In the GD test, 13 reference strains were correctly serotyped -with antigen from serovars 7 and 8 failing to react with any antiserum. The IHA methodology serotyped a total of 45 of 81 field isolates while the GD methodology serotyped a total of 48 isolates. For 29 isolates, the GD and IHA methods gave discordant results. It was concluded that the IHA is a good additional test for the serotyping of H. parasuis by the KRG scheme if the GD methodology fails to provide a result or shows unusual cross-reactions.

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“…20,21 Only limited information is available on the virulence determinants of H. parasuis. 4,15 A recent report suggested the existence of a putative hemolysin gene operon hhdBA, which has been found only in virulent reference serovars and could be a novel virulence factor for H. parasuis. 18 In the current study, PCR detection of hhdBA genes from H. parasuis reference strains appeared to differ from the previous results (Table 1).…”

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confidence: 99%

Detection of a putative hemolysin operon, hhdBA, of Haemophilus parasuis from pigs with Glässer disease

Assavacheep

Turni

2012

J VET Diagn Invest

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The aim of the current study was to investigate whether polymerase chain reaction amplification of 16S ribosomal (r)RNA and a putative hemolysin gene operon, hhdBA, can be used to monitor live pigs for the presence of Haemophilus parasuis and predict the virulence of the strains present. Nasal cavity swabs were taken from 30 live, healthy, 1- to 8-week-old pigs on a weekly cycle from a commercial Thai nursery pig herd. A total of 27 of these pigs (90%) tested positive for H. parasuis as early as week 1 of age. None of the H. parasuis–positive samples from healthy pigs was positive for the hhdBA genes. At the same pig nursery, swab samples from nasal cavity, tonsil, trachea, and lung, and exudate samples from pleural/peritoneal cavity were taken from 30 dead pigs displaying typical pathological lesions consistent with Glässer disease. Twenty-two of 140 samples (15.7%) taken from 30 diseased pigs yielded a positive result for H. parasuis. Samples from the exudate (27%) yielded the most positive results, followed by lung, tracheal swab, tonsil, and nasal swab, respectively. Out of 22 positive samples, 12 samples (54.5%) harbored hhdA and/or hhdB genes. Detection rates of hhdA were higher than hhdB. None of the H. parasuis–positive samples taken from nasal cavity of diseased pigs tested positive for hhdBA genes. More work is required to determine if the detection of hhdBA genes is useful for identifying the virulence potential of H. parasuis field isolates.

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A search for virulence genes of Haemophilus parasuis using differential display RT-PCR

Cited by 50 publications

References 22 publications

Haemophilus parasuis and Glässer's disease in pigs: a review

Haemophilus parasuis and Glässer's disease in pigs: a review

Comparison of the indirect haemagglutination and gel diffusion test for serotyping Haemophilus parasuis

Detection of a putative hemolysin operon, hhdBA, of Haemophilus parasuis from pigs with Glässer disease

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