A seamless system for the collection and cultivation of extremophiles from deep-ocean hydrothermal vents

Malahoff, Alexander; Gregory, T.; Bossuyt, A.; Donachie, Stuart P.; Alarn, M.

doi:10.1109/joe.2002.804058

Cited by 13 publications

(4 citation statements)

References 17 publications

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“…For the study and isolation of piezophilic microorganisms also sampling and incubation devices were developed and applied which allow processing of microbiological samples without depressurization (Kato, 2006 andreferences therein, Parkes et al, 2009). Two-phase batch incubation techniques with gas enrichment resulting from free-gas have also been described (Berberich et al, 2000;Boonyaratanakornkit et al, 2007;Kaneshiro and Clark, 1995;Knutson et al, 1999;Malahoff et al, 2002;Miller et al, 1989;Nelson et al, 1992). Further, several systems for high-pressure fed-batch or continuous incubation were designed and described (Houghton et al, 2007;Jannasch et al, 1996;Wirsen and Molyneaux, 1999).…”

Section: Discussionmentioning

confidence: 99%

High‐pressure systems for gas‐phase free continuous incubation of enriched marine microbial communities performing anaerobic oxidation of methane

Deusner

Meyer

Ferdelman

2009

Biotech & Bioengineering

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Novel high-pressure biotechnical systems that were developed and applied for the study of anaerobic oxidation of methane (AOM) are described. The systems, referred to as high-pressure continuous incubation system (HP-CI system) and high-pressure manifold-incubation system (HP-MI system), allow for batch, fed-batch, and continuous gas-phase free incubation at high concentrations of dissolved methane and were designed to meet specific demands for studying environmental regulation and kinetics as well as for enriching microbial biomass in long-term incubation. Anoxic medium is saturated with methane in the first technical stage, and the saturated medium is supplied for biomass incubation in the second stage. Methane can be provided in continuous operation up to 20 MPa and the incubation systems can be operated during constant supply of gas-enriched medium at a hydrostatic pressure up to 45 MPa. To validate the suitability of the high-pressure systems, we present data from continuous and fed-batch incubation of highly active samples prepared from microbial mats from the Black Sea collected at a water depth of 213 m. In continuous operation in the HP-CI system initial methane-dependent sulfide production was enhanced 10- to 15-fold after increasing the methane partial pressure from near ambient pressure of 0.2 to 10.0 MPa at a hydrostatic pressure of 16.0 MPa in the incubation stage. With a hydraulic retention time of 14 h a stable effluent sulfide concentration was reached within less than 3 days and a continuing increase of the volumetric AOM rate from 1.2 to 1.7 mmol L(-1) day(-1) was observed over 14 days. In fed-batch incubation the AOM rate increased from 1.5 to 2.7 and 3.6 mmol L(-1) day(-1) when the concentration of aqueous methane was stepwise increased from 5 to 15 mmol L(-1) and 45 mmol L(-1). A methane partial pressure of 6 MPa and a hydrostatic pressure of 12 MPa in manifold fed-batch incubation in the HP-MI system yielded a sixfold increase in the volumetric AOM rate. Over subsequent incubation periods AOM rates increased from 0.6 to 1.2 mmol L(-1) day(-1) within 26 days of incubation. No inhibition of biomass activity was observed in all continuous and fed-batch incubation experiments. The organisms were able to tolerate high sulfide concentrations and extended starvation periods.

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Section: Discussionmentioning

confidence: 99%

High‐pressure systems for gas‐phase free continuous incubation of enriched marine microbial communities performing anaerobic oxidation of methane

Deusner

Meyer

Ferdelman

2009

Biotech & Bioengineering

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“…Metatranscriptomics was also recently used to study microbial responses to the Deep lack thereof) for responding to such changes. Given that average lifetimes of prokaryotic 89 transcripts can be on the order of several minutes (Wang, et water sample in situ from 120m depth (Feike, et al, 2012), samplers that collect hydrothermal 104 vent fluids (e.g., Malahoff, et al, 2002, Phillips, et al, 2003, Taylor, et al, 2006, AUV…”

mentioning

confidence: 99%

Comparison of Niskin vs. in situ approaches for analysis of gene expression in deep Mediterranean Sea water samples

Edgcomb

Taylor

Pachiadaki

et al. 2016

Deep Sea Research Part II: Topical Studies in Oceanography

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“…Malahoff et al. fabricated a seamless system comprising a submersible-mounted sampler, a helium-activated sample transfer system and a series of stainless steel bioreactors ( Malahoff et al., 2002 ).…”

Section: Development Of Deep-sea Equipmentmentioning

confidence: 99%

Role of deep-sea equipment in promoting the forefront of studies on life in extreme environments

et al. 2021

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Summary The deep-sea environment creates the largest ecosystem in the world with the largest biological community and extensive undiscovered biodiversity. Nevertheless, these ecosystems are far from well known. Deep-sea equipment is an indispensable approach to research life in extreme environments in the deep-sea environment because of the difficulty in obtaining access to these unique habitats. This work reviewed the historical development and the state-of-the-art of deep-sea equipment suitable for researching extreme ecosystems, to clarify the role of this equipment as a promoter for the progress of life in extreme environmental studies. Linkages of the developed deep-sea equipment and the discovered species are analyzed in this study. In addition, Equipment associated with researching the deep-sea ecosystems of hydrothermal vents, cold seeps, whale falls, seamounts, and oceanic trenches are introduced and analyzed in detail. To clarify the thrust and key points of the future promotion of life in extreme environmental studies, prospects and challenges related to observing equipment, samplers, laboratory simulation systems, and submersibles are proposed. Furthermore, a blueprint for the integration of in situ observations, sampling, controllable culture, manned experiments in underwater environments, and laboratory simulations is depicted for future studies.

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A seamless system for the collection and cultivation of extremophiles from deep-ocean hydrothermal vents

Cited by 13 publications

References 17 publications

High‐pressure systems for gas‐phase free continuous incubation of enriched marine microbial communities performing anaerobic oxidation of methane

High‐pressure systems for gas‐phase free continuous incubation of enriched marine microbial communities performing anaerobic oxidation of methane

Comparison of Niskin vs. in situ approaches for analysis of gene expression in deep Mediterranean Sea water samples

Role of deep-sea equipment in promoting the forefront of studies on life in extreme environments

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